Increase in Oxidized Proteins in Theileria sergenti-Infected Erythrocyte Membrane.

Yagi, Yukio; Thongnoon, Prasobporn; Shiono, Hiroki; Chikayama, Yukio

doi:10.1292/jvms.64.623

Cited by 29 publications

(19 citation statements)

References 19 publications

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“…A value of MFI of DCFH oxidation is greatly accelerated by the production of H 2 O 2 and the presence of peroxidase. More recently, we actually found that rising levels of oxidized proteins in the RBC membrane were coincident with the onset of anemia in T. sergenti experimental infection (Yagi et al 2002). Moreover, increased MDA-TBARS in RBCs was accompanied by the decrease of PCV in all infected cattle, indicating that lipid peroxidation of the RBC membrane also occurred in the anemic cattle.…”

Section: Discussionmentioning

confidence: 80%

Oxidative damage and phosphatidylserine expression of red blood cells in cattle experimentally infected with Theileria sergenti

et al. 2003

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In order to elucidate the mechanism of anemia in Japanese bovine theileriosis, we investigated the oxidative alteration of red blood cells (RBCs) in cattle infected with Theileria sergenti. As an index of RBC oxidation, the levels of 2¢,7¢-dichlorofluorescin-diacetate (DCFH) oxidation and malondialdehyde-thiobarbituric acid reactive substances (MDA-TBARS), and phosphatidylserine (PS) expression accompanying anemia were examined in experimentally infected cattle. Before the development of anemia, the concentrations of DCFH oxidation and MDA-TBARS were low, and PS expression on the surfaces of RBCs was hardly seen. However, during the onset of anemia, these levels began to increase remarkably in proportion to the decrease of packed cell volume and the increase of parasitemia in all infected cattle. During the serious stage of anemia, these oxidative indices reached their maximum values. Our findings indicate that oxidative damage and loss of membrane asymmetry in RBCs are related to the development of anemia in T. sergenti infection. This oxidative damage to the RBCs might play an important role in the pathogenesis of anemia in Japanese bovine theileriosis.

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Section: Discussionmentioning

confidence: 80%

Oxidative damage and phosphatidylserine expression of red blood cells in cattle experimentally infected with Theileria sergenti

et al. 2003

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“…Other factors would include protease enzymes (Somerville, et al, 1998;Stockham et al, 2000;Shiono et al, 2004), oxygen radicals, increased oxidative proteins in the RBCs membranes and oxidative damage (Shiono et al, 2002;2003). The main pathogenesis of T. annulata and T.serjenti were reported by damaging effects on RBCs (Asri and Dalir-Naghadeh, 2006).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the erythrocytes and leucocyte alterations in cows infected with Theileria annulata

Ramin¹,

Asri-Rezaie²,

Hemati³

et al. 2011

Acta vet. (Beogr.)

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The percentages of anemia, parasitemia and leucocyte changes were investigated in bovine theileriosis in the Northwest of Iran. The hematological values in 91 cows with theileriosis were compared with 19 healthy ones. The disease was confirmed by the presence of parasites in the peripheral blood smear test. Five mLs of blood from the jugular vein was collected and mixed with EDTA. Hematological parameters and parasitemia were assessed by current laboratory methods. The mean for hematocrit (PCV), hemoglobin (Hb), total leucocytes counts (WBC) and erythrocyte count (RBCs) were 22.1%, 6.8 mg/dL, 7075/μL and 4x106/μL, respectively. Mean total protein, absolute lymphocyte, neutrophil, eosiniphil counts, MCV, MCH and MCHC were 6.4 g/dL, 3898, 2746, 136/μL, 55.5 fl, 16.7 Pg and 30.5 g/dL, respectively. Mean concentrations for PCV, Hb, RBCs, and MCHC were significantly (p<0.05) lower and MCV was higher than the control group. To determine the severity of anemia in theileriosis, PCV was classified into cows without anemia (>24%), mild (20-23.99%), moderate (12-19.99%) and severe anemia (<11.99%). Therefore, the frequency and percentage of infected cows were 44 (48.4%), 14 (15.3%), 23 (25.3%) and 10 cases (11%), respectively (p<0.01). The percentages of parasitemia among 4 groups (1.23, 3.86, 9.64 and 34.2%, respectively) were also significantly (p<0.01) different. Among the indices under study, mean RBCs and Hb showed significant differences (p<0.01) in all types of anemia. The types of anemia were varied from normocytic hypochromic to macrocytic hypochromic. Negative correlations were found (p<0.01) between parasitemia/PCV (r=-0.74), parasitemia/Hb (r=-0.64) and parasitemia/RBCs (r=-0.63). It is concluded that anemia would be the main clinical sign in theileriosis. Type of anemia varied based on the severity of the anemia. Hematocrit, Hb, RBCs MCV and MCHC are the main indices affected in theileriosis. The majority of the infected cows were affected by moderate anemia except for 11%, in that they showed severe anemia with 34.2% parasitemia. Thus, the assessment of RBCs indices will lead to the prognosis and appropriate treatment strategies

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“…We have previously reported that PS molecules are exposed to the outer membrane of the RBC during the onset of anaemia in experimentally produced T. sergenti infection in cattle (Shiono et al, 2003), but this phenomenon alone cannot account for such a fast and massive removal of RBC from the blood circulation. We have also reported that the RBC membrane and intracellular components are remarkably oxidized in anaemic cattle infected with T. sergenti, which correlated closely with the progression of anaemia in each individual (Shiono et al, 2001(Shiono et al, , 2003Yagi et al, 2002). Based on this evidence, we hypothesized that oxidative stress might play an important role in the pathogenesis of anaemia in T. sergenti infection.…”

Section: Discussionmentioning

confidence: 66%

Accelerated Binding of Autoantibody to Red Blood Cells with Increasing Anaemia in Cattle Experimentally Infected with Theileria sergenti

Shiono

Yagi

Kumar

et al. 2004

Journal of Veterinary Medicine, Series B

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Anaemia is the most important clinical manifestation in cattle infected with Theileria sergenti. In order to determine the mechanism of red blood cells (RBC) destruction in anaemic cattle, we studied the binding of autoantibody (IgG) to RBC during the development of anaemia in T. sergenti infection. The low levels of IgG-bound RBC before the development of anaemia were triggered in proportion with the progression of anaemia and parasitaemia. Our results suggest an accelerated destruction of RBC in anaemic cattle by IgG-dependent phagocytosis.

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Increase in Oxidized Proteins in Theileria sergenti-Infected Erythrocyte Membrane.

Cited by 29 publications

References 19 publications

Oxidative damage and phosphatidylserine expression of red blood cells in cattle experimentally infected with Theileria sergenti

Oxidative damage and phosphatidylserine expression of red blood cells in cattle experimentally infected with Theileria sergenti

Evaluation of the erythrocytes and leucocyte alterations in cows infected with Theileria annulata

Accelerated Binding of Autoantibody to Red Blood Cells with Increasing Anaemia in Cattle Experimentally Infected with Theileria sergenti

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