Increase in plasma high-density lipoprotein concentration following complete androgen blockage in men with prostatic carcinoma

Moorjani, Sital; Dupont, André; Labrie, Fernand; Lupien, Paul J.; Brun, Daniel; Gagné, Claude; Giguère, M.; Bélanger, Alain

doi:10.1016/0026-0495(87)90183-1

Cited by 204 publications

(112 citation statements)

References 31 publications

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“…Cholesterol and triglyceride level measurements in plasma and lipoprotein fractions were performed with a Technicon RA-analyzer (Bayer, Etobicoke, Canada) using enzymatic methods, as previously described. 17 Plasma VLDLs were isolated by ultracentrifugation, and the HDL fraction was obtained by precipitation of LDLs from the infranatant with heparin and MnCl 2 . 18 The cholesterol content of the infranatant was measured before and after precipitation, and the concentration of LDL cholesterol was obtained by difference.…”

Section: Plasma Lipid-lipoprotein Measurementsmentioning

confidence: 99%

Hypertrophy and hyperplasia of abdominal adipose tissues in women

et al. 2007

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Objective: To examine the expression of selected transcription factors involved in adipogenesis and genes related to lipid metabolism in abdominal subcutaneous and omental fat tissue. Research design and methods: We obtained subcutaneous and omental adipose tissue samples from 40 women undergoing abdominal hysterectomies (age: 4775 years; BMI 27.975.3 kg/m 2 ). We measured isolated adipocyte size and metabolism, and detailed measures of body fat accumulation and body fat distribution were obtained (dual-energy X-ray absorptiometry and computed tomography, respectively). Results: Adipocyte size of both subcutaneous and omental fat were increased with higher body fat mass values, with similar regression slopes in each compartment. In contrast, with higher body fat mass values, fat accumulation was progressively higher in the subcutaneous than in the visceral fat compartment, suggesting hyperplasia in the subcutaneous fat compartment. Messenger RNA levels of CEBPa, PPARg2, SREBP1c and genes related to lipid metabolism (LPL, FABP4, DGAT1, DGAT2, PLIN and HSL) were significantly higher in subcutaneous than in omental fat tissue (Pp0.001 for all). Only subcutaneous expression of these genes tracked with obesity levels as reflected by significant positive associations between subcutaneous fat CEBPa, SREBP1c and DGAT2 expression and total body fat mass (r ¼ 0.37, r ¼ 0.41, r ¼ 0.57, respectively, Pp0,05), fat percentage (r ¼ 0.40, r ¼ 0.39, r ¼ 058, respectively, Pp0,05) and subcutaneous adipose tissue area (r ¼ 0.36, r ¼ 0.38, r ¼ 0.58, respectively, Pp0,05). Omental adipose tissue expression levels of these genes were not significantly related to adiposity measures. Conclusions: These results show that in obese women, hyperplasia is predominant in the subcutaneous fat depot, whereas fat cell hypertrophy is observed both in the omental and subcutaneous compartments.

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Section: Plasma Lipid-lipoprotein Measurementsmentioning

confidence: 99%

Hypertrophy and hyperplasia of abdominal adipose tissues in women

et al. 2007

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“…18,21 Apolipoprotein (apo) B concentrations were measured in plasma by the rocket immunoelectrophoretic method of Laurell, 22 as previously described. 23 Serum standards were prepared and calibrated against reference sera obtained from the Center for Disease Control (Atlanta, GA, USA). Plasma glucose concentration was enzymatically measured.…”

Section: Biological Variablesmentioning

confidence: 99%

Features of the metabolic syndrome are modulated by an interaction between the peroxisome proliferator-activated receptor-delta −87T>C polymorphism and dietary fat in French-Canadians

et al. 2006

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The metabolic syndrome (MS) is influenced by genetic and environmental factors. Peroxisome proliferator-activated receptor delta (PPAR-delta), a transcription factor involved in lipid metabolism, is a candidate gene for the MS. Objective: We verified whether genetic variants in this gene are associated with the MS and whether dietary fatty acids interact with the À87T4C polymorphism. Methods: By direct sequencing, we identified 15 variants in the PPAR-delta gene and analyses were pursued with the À87T4C polymorphism for 340 subjects. Results: Metabolic variables were comparable among each genotype group. The À87T4C polymorphism, fat intake and the interaction accounted, respectively for 2.2, 1.9 and 1.5% of the variance in high-density lipoprotein cholesterol (HDL-C) levels (Po0.05) (age, sex and energy intake were included into the model). The total cholesterol/HDL-C ratio was also modulated by a gene-diet interaction and by the -87T4C polymorphism (Po0.05). No gene-diet interaction effects were observed for other features of the MS. The age-and sex-adjusted odds ratio (OR) of exhibiting three or more features of the MS when carrying the À87C allele was 0.62 (P ¼ 0.04) compared to -87T/T. However, in subjects consuming less than 34.4% of energy from fat (median of fat consumption), the OR in carriers of the À87C allele was of 0.42 (P ¼ 0.008). Conclusion: These data suggest that the PPAR-delta À87T4C polymorphism may be associated with a lower risk to exhibit the MS and this association is influenced by dietary fat intake.

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“…Very low density lipoproteins (VLDLs) were isolated by ultracentrifugation (d Ͻ 1.006 g/ml) and the high-density lipoprotein cholesterol (HDL-C) fraction was obtained after precipitation of lowdensity lipoprotein (LDL) in the infranatant (d Ͼ 1.006 g/ ml) with heparin and MnCl 2 (Després et al 1991;Moorjani et al 1987). Apolipoprotein (apo) B concentrations were measured in plasma and infranatant (LDL-apo B; d Ͼ 1.006 g/ml) by the rocket immunoelectrophoretic method of Laurell (1966), as previously described (Krasinski et al 1990).…”

Section: Lipoprotein and Lipid Measurementsmentioning

confidence: 99%