Increase in ribosomal proteins activity: Translational reprogramming inVanilla planifoliaJacks., againstFusariuminfection

Abstract

BackgroundUpon exposure to unfavorable environmental conditions, plants need to respond quickly to maintain their homeostasis. For instance, physiological, biochemical and transcriptomical changes must occur during interactions with pathogens, this causing the triggering of pathogen- and plant-derived molecules. In the case of Vanilla planifolia Jacks., a worldwide economically important crop, it is susceptible to Fusarium oxysporum f. sp. vanillae. This pathogen causes root and stem rot in vanilla plants that finally leads to plant death. To further investigate how vanilla plants respond at the transcriptional level upon infection with F.oxysporum f. sp. vanillae, we employed the RNA-Seq approach to analyze the dynamics of whole-transcriptome changes during two-time frames of the infection.ResultsAnalysis of global gene expression profiles indicated that a major transcriptional change occurs at 2 dpi, in comparison to 10 dpi, whereas 3420 genes were found with a differential expression at 2 dpi, only 839 were identified at 10 dpi. The analysis of the transcriptional profile at 2 dpi suggests that vanilla plants prepare to counter the infection by gathering a pool of translational regulation-related transcripts.ConclusionsWe propose that the plant-pathogen interaction at early stages causes a transcriptional reprogramming coupled with a translational regulation. Altogether, this study provides the identification of molecular players that could help to fight the most damaging disease of vanilla, where ribosomal proteins and regulation of the translational mechanism are critical. These are insights into the defense responses of V. planifolia Jacks., providing the basis for the understanding of the plant early response towards biotic stress.

Transcriptome analysis of responses in Brachypodium distachyon overexpressing the BdbZIP26 transcription factor

“…One that is probably less commonly thought of is the role ribosomal proteins have in post-transcriptional and translational regulation [55][56][57]. The differential expression of specific ribosomal proteins in response to abiotic and biotic stress has been previously reported [55,56,58,59]. Interestingly, in this study, ribosomal proteins were more highly represented in the up-regulated (41 up-regulated, nine downregulated) DEGs.…”

Proteome Mapping of South African Cassava Mosaic Virus-Infected Susceptible and Tolerant Landraces of Cassava

“…The 40S ribosomal protein S4 (Manes.03G095200) had ratios of 1.59 in T200 and −1.06 in TME3. An increase in transcript levels of this protein was observed in Vanilla planifolia Jacks infected with fungi [ 51 ], whereas there was a decrease in transcript in Arabidopsis 48 h after inoculation with A. tumefaciens [ 52 ]. The 60S ribosomal protein L13 (Manes.07G017100) was under-expressed in T200 (−3.86) and over-expressed in TME3 (2.11).…”

LC–MS Based Draft Map of the Arabidopsis thaliana Nuclear Proteome and Protein Import in Pattern Triggered Immunity

“…On the other hand, the ribosomal proteins with decreased abundance in the nucleus under flg22 and nlp20 compared to control (nine proteins) have a repressed function in the nucleus during the Arabidopsis immune response. In a previous study the ribosomal protein transcripts were investigated in Vanilla planifolia when infected with Fusarium oxysporum ( Solano de la Cruz et al, 2019 ). Seven ribosomal proteins that showed an increase in abundance in the nucleus after elicited immunity in our study also showed an increase in their transcript expression patterns in Vanilla after 2days of Fusarium infection.…”