Increase of ICSI efficiency with hyaluronic acid binding sperm for low aneuploidy frequency in pig

Park, Chun Young; Uhm, Sang Jun; Song, Seung-Hun; Kim, Kwang Sung; Hong, Seung Bum; Chung, Kyuha; Park, Chankyu; Lee, Hoon Taek

doi:10.1016/j.theriogenology.2005.01.010

Cited by 35 publications

(22 citation statements)

References 39 publications

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“…It may be claimed that ICSI produces lower blastocyst rates, but the ICSI technologies have been intensively improved in farm animals, especially for porcine studies, and in our laboratory, we have obtained 20-30% rates of blastocyst development [32][33][34]. Furthermore, a previous attempt at porcine ICSI provided higher than normal numbers of diploid embryos and lower numbers of mixoploid embryos than IVF [31]. Therefore, more research should be concentrated on improving the developmental capacity of porcine oocytes matured in vitro, possibly using a defined IVM culture medium [32,33] with subsequent ICSI [32][33][34], and on development of a defined IVC culture medium such as PZM-4 [2,10].…”

Section: Discussionmentioning

confidence: 65%

“…Traditional IVF has produced high rates of polyspermic fertilization [2][3][4][5]26] and aneuploidy, especially mixoploidy in subsequently cultured bovine and porcine embryos [28][29][30][31]. In order to remove the incidence of polyspermic fertilization in IVF of porcine oocytes, we attempted ICSI of in vitro matured porcine oocytes and obtained 20-30% blastyocyst rates after IVC [32][33][34].…”

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confidence: 99%

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In Vitro Culture Conditions Using Chemically Defined Media for In Vitro Matured and Intracytoplasmically Inseminated Porcine Oocytes

Kamiya

Kobayashi

Fukui

2006

Journal of Reproduction and Development

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Abstract. The present study investigated in vitro culture methods [droplet and Well of the Well (WOW)] using semi-defined and defined media [modified porcine zygote medium (mPZM)] and the additional effects of insulin on in vitro matured and intracytoplasmically inseminated porcine oocytes. In Experiment 1, in vitro matured and intracytoplasmically inseminated porcine oocytes were cultured for 6 days in the following four groups: 1) mPZM-3 (containing bovine serum albumin) + droplet (30 µl), 2) mPZM-3 + WOW, 3) mPZM-4 (containing polyvinyl alcohol) + droplet, and 4) mPZM-4+ WOW. The culture media (mPZM-3 and mPZM-4) and methods (droplet and WOW) did not significantly affect the cleavage rate, but the blastocyst rate of the oocytes cultured in mPZM-3 was significantly (P<0.01) higher than that of mPZM-4 (20.1 and 9.4%, respectively). The blastocyst rates as percentages of the cleaved oocytes (51.8 and 16.9%) and the hatched blastocyst rate as percentages of the number of blastocysts (12.3 and 2.2%) were also significantly (P<0.01) higher in mPZM-3 compared with those in mPZM-4. There was significant interaction (P<0.05) between the two main factors; the effects of the culture media and methods on the rate of hatched blasyocysts as percentages of the blastocysts produced and, the hatched blastocyst rate (20.3%) as percentages of the number of blastocysts produced in mPZM-3 were significantly (P<0.05) higher than in the other groups. In Experiment 2, the additional effects of insulin (100 ng/ml) in mPZM-3 and mPZM-4 media was investigated in the WOW culture system. Insulin addition did not improve cleavage, blastocyst formation, or the number of cells in blastocysts. However, as in Experiment 1, mPZM-3 resulted in a significantly higher blastocyst rate as percentages of the cleaved oocytes than mPZM-4 (33.9 and 18.4%). These results indicate that a chemically defined medium (mPZM-4) needs to be improved to provide more suitable culture conditions for in vitro development of in vitro matured and intracytoplasmically inseminated porcine oocytes. However, the WOW system may be a useful IVC method for blastocyst development of in vitro matured porcine oocytes following ICSI when a semidefined medium (mPZM-3) is used.

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Section: Discussionmentioning

confidence: 65%

mentioning

confidence: 99%

In Vitro Culture Conditions Using Chemically Defined Media for In Vitro Matured and Intracytoplasmically Inseminated Porcine Oocytes

Kamiya

Kobayashi

Fukui

2006

Journal of Reproduction and Development

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“…In spermiogenesis with elimination of cytoplasm and HspA2 synthesis, the sperm plasma membrane undergoes maturation remodeling that facilities the formation of the sites for zona pellucida and hyaluronic acid binding in mature sperm [33]. Thus, the sperms selected by HBA could give information on sperm biomarkers located on plasma membrane in addition to morphology and only mature sperms can bind to HA [34]. In addition, Prinosilova et al [35] reported that the Tygerberg normal morphology increased in the HA-bound fractions and significantly improved in the infertile subgroups containing oligozoospermic to high normozoospermic ranges.…”

Section: Discussionmentioning

confidence: 99%

Hyaluronic Acid Binding Assay Is Highly Sensitive to Select Human Spermatozoa with Good Progressive Motility, Morphology, and Nuclear Maturity

Ghaleno¹,

Valojerdi

Chehrazi³

et al. 2015

Gynecol Obstet Invest

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Objective: This study was performed to evaluate the correlation of hyaluronic acid binding assay (HBA) with conventional semen parameters, lipid peroxidation (LPO), intracellular reactive oxygen species (ROS), DNA fragmentation (DF), DNA maturity and mitochondrial membrane potential (MMP) level in human spermatozoa. Methods: The semen samples were obtained from 98 patients. The seminal plasma was separated for the study of LPO, and the pellet was employed for evaluation of intracellular ROS, DF, nuclear maturity (sperm chromatin structure assay) and MMP through flowcytometry. Results: The correlation and strength of HBA with respect to the studied parameters were estimated by the Pearson coefficient and multiple liner regression tests. While HBA indicated a positive correlation with progressive motility (β-coefficients = 0.449, p < 0.05) and normal morphology (β-coefficients = 2.722, p < 0.01), it had only negative relationship with DNA integrity (high DNA stain ability; β-coefficients = -0.517, p < 0.05). HBA also did not show any important correlation with other conventional and intracellular sperm parameters. Conclusions: The HBA is sensitive to morphological integrity, high progressive motility and nuclear maturation. Nonetheless, HBA is not a reliable test for prediction of sperm intracellular ROS, DF and MMP risks and healthy spermatozoa selection.

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“…Recently, aneuploidies in early pig embryos were investigated for the first time using Comparative Genomic Hybridization (CGH) [Hornak et al, 2009]. FISH has also been used to analyze the activation of ribosomal RNA genes [Bjerregaard et al, 2007] or the ability of hyaluronic-acid-binding sperm to increase the efficiency of intracytoplasmic sperm injection [Park et al, 2005]. There are few papers concerning oncocytogenetics in domestic animals.…”

Section: The Case Of Pig (Sus Scrofa Domestica)mentioning

confidence: 99%

Fluorescence in situ Hybridization Applied to Domestic Animal Cytogenetics

Rubeš

Pinton

Bonnet-Garnier

et al. 2009

Cytogenet Genome Res

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The aim of this article is not to present an exhaustive review of molecular cytogenetics applications in domestic animal species, but more to illustrate the considerable contribution of these approaches in diagnostics and research in economically important species. A short presentation of the main applications of molecular cytogenetics in humans points out the domains in which it has become an essential tool and underlines the specificities attached to this species in comparison to farm animals. This article is devoted to outlining the current resources available in domestic species and to some examples of fluorescence in situ hybridization applications in the cattle, pig, horse and avian species. From a clinical point of view, these examples illustrate the advantages of FISH for the study of chromosomal abnormalities (identification, characterization and estimation of their effects). Other applications of molecular cytogenetics are also illustrated, particularly ZOO-FISH, an approach which allows the determination of chromosome homologies between species. Finally, a specific emphasis was placed on the usefulness of molecular cytogenetics for the analysis of species such as poultry, which harbour a complex karyotype.

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Increase of ICSI efficiency with hyaluronic acid binding sperm for low aneuploidy frequency in pig

Cited by 35 publications

References 39 publications

In Vitro Culture Conditions Using Chemically Defined Media for In Vitro Matured and Intracytoplasmically Inseminated Porcine Oocytes

In Vitro Culture Conditions Using Chemically Defined Media for In Vitro Matured and Intracytoplasmically Inseminated Porcine Oocytes

Hyaluronic Acid Binding Assay Is Highly Sensitive to Select Human Spermatozoa with Good Progressive Motility, Morphology, and Nuclear Maturity

Fluorescence in situ Hybridization Applied to Domestic Animal Cytogenetics

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