Increased bone morphogenetic protein-6 in follicular fluid and granulosa cells may correlate with fertilization and embryo quality in humans

Liang, Ying; Cao, Qian; Gao, Xing; Du, Hongyin

doi:10.3892/etm.2017.4603

Cited by 7 publications

(3 citation statements)

References 40 publications

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“…But there was no significant difference between mothers with daughters and mothers with sons in PPARγ protein level (P=0.12). These two proteins are the most important indicators for determining fertility (17,44,45). Therefore, in the study, their effect on determining the gender of the child was evaluated.…”

Section: Discussionmentioning

confidence: 99%

The effect of cellular and physiological indicators on gender determination

Haje

2021

Cell Mol Biol (Noisy-le-grand)

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Gender determination, in addition to having special value to parents, has particular importance in sex-linked diseases. This study aimed to investigate the cellular indicators (i.e. BMP-6 protein and PPAR? protein expression levels in granulosa cells) and the physiological indicators on gender determination. For this purpose, on 68 infertile patients referred to the clinic, ovarian stimulation was performed by different protocols and then ruptured by different HCG. Follow-up of patients was performed after they became pregnant after five months. U/S was done for knowing the gender of the baby then after labor rechecked another time. Also, granulosa-luteal cells (GLCs) were isolated from the follicular fluid of 68 women participating in the study. BMP-6 protein and PPAR? protein were measured using Western blotting. Results showed that the total number of delivered babies was 68, 41 males (60.3%) and 27 females (39.7%). About physiological indicators results, there was no significant association between the age of the mother and sex of the baby (P=0.934). No significant association was detected between the month during which the conception occurred and the sex of the baby (P=0.734). The same result was obtained for the follicle side (P=0.236), and follicle size (P=0.659), there was no significant association between the sex of the baby with the following factors: protocol of treatment (P=0.417), IVF after HCG (P=0.237), HCG type (P=0.572), parity (P=0.282), and type of infertility (P=0.376). The cellular indicators results showed that the BMP-6 protein level in granulosa cells of mothers with daughters was almost twice as high as mothers with sons (P=0.043). But there was no significant difference between mothers with daughters and mothers with sons in PPAR? protein level (P=0.12). It can be concluded that except for BMP-6 protein level, none of the cellular and physiological indicators affects gender determination. Therefore, this cell indicator can probably be evaluated as an effective indicator in determining gender.

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Section: Discussionmentioning

confidence: 99%

The effect of cellular and physiological indicators on gender determination

Haje

2021

Cell Mol Biol (Noisy-le-grand)

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“…BMP‐6 is expressed in oocytes and cumulus cells and acts by increasing AMH and FSH receptor expression, decreasing StAR and GDNF expression, suppressing luteinization by decreasing progesterone production, 6,35,36 so making their participation in good oocyte quality unquestionable. We demonstrated that BMP‐6 had relatively decreased gene expression in the group of infertile women with endometriosis and this finding could be better explored by studying the oocyte competence in this subgroup of women.…”

Section: Discussionmentioning

confidence: 99%

BMP‐6 and SMAD4 gene expression is altered in cumulus cells from women with endometriosis‐associated infertility

Conto

Matte

Cunha-Filho

2020

Acta Obstet Gynecol Scand

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Introduction Oocyte competence and quality depend on communication between the oocyte and the cumulus and theca cells. In the preantral phase, the members of the transforming growth factor β (TGF‐β) superfamily are responsible for this communication and play an important role in folliculogenesis. Members of the TGF‐β superfamily are related to endometriosis (overexpression in the ectopic endometrium); however, few studies have explored these proteins as influencing fertility in endometriosis. Considering endometriosis‐related infertility and to better understand the role of the TGF‐β superfamily members in the antral phase in women with endometriosis, this research investigated the gene expression of the genes for ligands AMH, BMP‐6, GDF‐9, INHA, INHBB, and TGFβ3; receptors AMHR2, BMPR2, and TGFβR3; and intracellular signalling: SMAD3 and SMAD4. Material and methods The gene expression of AMH, BMP‐6, GDF‐9, INHA, INHBB, TGFβ3, AMHR2, BMPR2, TGFβR3, SMAD3, and SMAD4 in cumulus cells was investigated through quantitative real‐time PCR in a case‐control study including infertile women with and without peritoneal endometriosis undergoing in vitro fertilization. Results Age and outcomes of assisted reproduction were similar between the groups (P > .05). However, women with endometriosis showed reduced expression of BMP‐6 and SMAD4 (P < .05) in cumulus cells compared with the control group, other genes did not present altered gene expression in women with endometriosis (P > .05). Conclusions The reduced expression of BMP‐6 and SMAD4 in women with peritoneal endometriosis compared with the control group indicates that granulosa (cumulus) cell function could be altered in these women.

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“…The inclusion criteria were patients who experienced IVF-ET due to infertility caused by tubal factors or male factors (varying degrees of oligospermia, asthenia, and teratozoospermia, but not azoospermia) or both, with an age ≤ 38 years, regular menstruation (cycle 21–35 days, period 2–8 days, menstrual volume 20–60 ml) 55 , basic FSH < 10 IU/L, first-cycle COS or repeated COS for 3–5 cycles. The exclusion criteria were the same as those in the previous study 56 : patients who were unsuitable for pregnancy due to conditions such as uterine abnormalities or serious heart diseases and who suffered from serious mental disorders, urinary and reproductive system infection, sexually transmitted diseases, endometriosis, uterine fibroids, tuberculosis, uterine adhesion, endometrial lesions, polycystic ovary syndrome, ovarian cyst, and hydrosalpinx. Patients who had been exposed to teratogenic radiation or poison and who were within the action period of the drugs were also excluded.…”

Section: Methodsmentioning

confidence: 99%

Effects of Bushen-Tiaojing-Fang on the pregnancy outcomes of infertile patients with repeated controlled ovarian stimulation

Hao

Zhao

et al. 2021

Sci Rep

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Bushen-Tiaojing-Fang (BSTJF) is commonly used to treat infertility. This study investigated the effects of BSTJF on the pregnancy outcomes of patients with repeated controlled ovarian stimulation (COS), on mitochondrial function, and on oxidative stress in ovarian granulosa cells (GCs) and follicular fluid (FF). The samples and clinical data of 97 patients, including 35 in the control group, 29 in the placebo group and 33 in the BSTJF group, were collected for this study. The mitochondrial ultrastructure, ATP content, mitochondrial DNA (mtDNA) number, 8-hydroxy-2-deoxyguanosine (8-OHdG), Mn-superoxide dismutase (Mn-SOD), glutathione peroxidase (GSH-Px) activity levels, and mRNA expression levels of Mn-SOD, GSH-Px, and nuclear factor erythroid-derived factor 2-related factor 2 (Nrf2) were analyzed. The high-grade embryo (P < 0.001), implantation (P = 0.033), and clinical pregnancy (P = 0.031) rates, as well as the ATP content (P = 0.014), mtDNA number (P = 0.035), GSH-Px activity (P = 0.004 in GCs and P = 0.008 in FF) and mRNA expression levels (P = 0.019), were significantly lower in the placebo group than in the control group, whereas the 8-OHdG content was significantly (P = 0.006 in FF) higher in the placebo group than in the control group. Compared with those in the placebo group, the high-grade embryo rate (P = 0.007), antioxidant enzyme activity (P = 0.037 and 0.036 in Mn-SOD; P = 0.047 and 0.030 in GSH-Px) and mRNA level (P < 0.001 in Nrf2, P = 0.039 in Mn-SOD and P = 0.002 in GSH-Px) were significantly higher in the BSTJF group, as were changes in mitochondrial ultrastructure, ATP (P = 0.040) and mtDNA number (P = 0.013). In conclusion, BSTJF can improve oxidative stress in patients with repeated COS and pregnancy outcomes.

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Increased bone morphogenetic protein-6 in follicular fluid and granulosa cells may correlate with fertilization and embryo quality in humans

Cited by 7 publications

References 40 publications

The effect of cellular and physiological indicators on gender determination

The effect of cellular and physiological indicators on gender determination

BMP‐6 and SMAD4 gene expression is altered in cumulus cells from women with endometriosis‐associated infertility

Effects of Bushen-Tiaojing-Fang on the pregnancy outcomes of infertile patients with repeated controlled ovarian stimulation

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