Increased brazzein expression by abiotic stress and bioreactor culture system for the production of sweet protein, brazzein

Han, Jong-Eun; Park, Yun-Ji; Lee, Hyoshin; Jeong, Yoo-Jin; Park, Soyoung

doi:10.1007/s11816-020-00625-6

Cited by 6 publications

(2 citation statements)

References 33 publications

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“…Secondary metabolite production by cell cultures is an alternative to chemical synthesis however; it may be advantageous only when the metabolites are biosynthesized in speci c plant tissues. Examples have been reported in carrot, mung beans, sa ower, sun ower, saffron, wormwood and yew (Caretto et al 2011;Malik et al 2011;Yue et al 2016;Almagro et al 2017;Han et al 2020;Moradi et al 2020). With the aim of increasing the perspectives and widening the horizons in this eld, the systems for the production of secondary metabolites using in vitro cell cultures are under particular consideration and critically studied (Sajc et al 2000, Zhong, 2001Ruffoni et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Elicitation of phenylpropanoids in maqui (Aristotelia chilensis [Mol.] Stuntz) plants micropropagated in photomixotrophic temporary immersion bioreactors (TIBs)

Trentini

Rojas

Gajardo

et al. 2021

Plant Cell Tiss Organ Cult

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A biotechnological system for the production of plants biomass and phenylpropanoids of maqui was developed in photomixotrophic TIBs. The in vitro maqui multiplication was evaluated using combinations of TDZ and BAP in TIBs 1L capacity. Treatment of MS basal supplemented with TDZ 1 mg/l shows the best results for the variables fresh weight and multiplication rate. Photomixotrophic conditions were standardized in media with 3%, 2%, 1%, 0% sucrose at a light intensity of 100 µM m − 2 s − 1 . The treatments reduced in sucrose (1% and 2%) and air supplemented with 0.4 MPa CO 2 do not differ signi cantly in biomass production (fresh weight per cluster of plants) compared to the control treatment with sucrose 3% and standard air.Treatment with ABA (1m/l) induced the production and accumulation of phenylpropanoids metabolites in maqui cultures bioreactors. Phenylpropanoids in in vitro biomass of maqui and culture medium from TIBs were determined in parallel with control samples from wild plants and mature fruits. After the third day of analysis, not signi cant differences in polyphenols and anthocyanin contents were veri ed between the treatments of maqui in TIBs + ABA and controls. The non-signi cant differences in the contents of polyphenols and anthocyanin were maintained until the 15 days of analysis. The antioxidant capacity comparing samples of maqui in bioreactors and wild plants showed no signi cant differences by the ORAC test from day 5 to day 15 of the study. Key MessageFor the rst time maqui micropropagation has been established in TIBs followed by elicitation of phenylpropanoids metabolites. Contents of metabolites from maqui in TIBs do not differ from those of wild fruits, while antioxidant capacity of phenylpropanoids was preserved during the study.

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Section: Discussionmentioning

confidence: 99%

Elicitation of phenylpropanoids in maqui (Aristotelia chilensis [Mol.] Stuntz) plants micropropagated in photomixotrophic temporary immersion bioreactors (TIBs)

Trentini

Rojas

Gajardo

et al. 2021

Plant Cell Tiss Organ Cult

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“…Yet, employing intact transgenic plants is required for these systems, which restricts their use for field cultivation. Han et al [90] recently developed a brazzein synthesis platform. To achieve this, they employed carrot cell suspension in a bioreactor.…”

Section: Brazzeinmentioning

confidence: 99%

Brazzein and Monellin: Chemical Analysis, Food Industry Applications, Safety and Quality Control, Nutritional Profile and Health Impacts

Saraiva

Carrascosa

Ramos

et al. 2023

Foods

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Recently, customers have been keener to buy products manufactured using all-natural ingredients with positive health properties, but without losing flavor. In this regard, the objective of the current study is to review the consumption of brazzein and monellin, their nutritional profiles and health effects, and their potential applications in the food industry. This poses challenges with sustainability and important quality and safety indicators, as well as the chemical processes used to determine them. To better understand the utilization of brazzein and monellin, the chemical analysis of these two natural sweet proteins was also reviewed by placing particular emphasis on their extraction methods, purification and structural characterization. Protein engineering is considered a means to improve the thermal stability of brazzein and monellin to enhance their application in food processing, especially where high temperatures are applied. When the quality and safety of these sweet proteins are well-investigated and the approval from safety authorities is secured, the market for brazzein and monellin as food ingredient substitutes for free sugar will be guaranteed in the future. Ultimately, the review on these two natural peptide sweeteners increases the body of knowledge on alleviating problems of obesity, diabetes and other non-communicable diseases.

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Brazzein protein production in transgenic carrot cells using air-lift bioreactor culture

Han

Lee

et al. 2022

Plant Biotechnol Rep

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Increased brazzein expression by abiotic stress and bioreactor culture system for the production of sweet protein, brazzein

Cited by 6 publications

References 33 publications

Elicitation of phenylpropanoids in maqui (Aristotelia chilensis [Mol.] Stuntz) plants micropropagated in photomixotrophic temporary immersion bioreactors (TIBs)

Elicitation of phenylpropanoids in maqui (Aristotelia chilensis [Mol.] Stuntz) plants micropropagated in photomixotrophic temporary immersion bioreactors (TIBs)

Brazzein and Monellin: Chemical Analysis, Food Industry Applications, Safety and Quality Control, Nutritional Profile and Health Impacts

Brazzein protein production in transgenic carrot cells using air-lift bioreactor culture

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