Increased Cell Proliferation and Gene Expression of Genes Related to Bone Remodeling, Cell Adhesion and Collagen Metabolism in the Periodontal Ligament of Unopposed Molars in Growing Rats

Dorotheou, Domna; Farsadaki, Vassiliki; Bochaton‐Piallat, Marie‐Luce; Giannopoulou, Catherine; Halazonetis, Thanos D.; Kiliaridis, Stavros

doi:10.3389/fphys.2017.00075

Cited by 8 publications

(8 citation statements)

References 38 publications

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“…The treatment of tylosin increased the expression of cell proliferation and cell adhesion associated genes and reduced the expression of cell cycle progression or apoptosis related genes. Increased expressions of cell proliferation and cell adhesion associated genes and reduced expressions of cell cycle progression or apoptosis related genes are also reported in previous studies (Dorotheou et al 2017;Hofmann et al 2015). The result of gene expression indicates that the tested concentration of tylosin has no adverse effects on general cell development.…”

Section: Discussionsupporting

confidence: 80%

Physiological influence of tylosin tartrate overdose in vivo in rats

et al. 2020

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The aim of this study was to evaluate the physiological effects of tylosin in rats. Tylosin was administered orally to pubertal male and female rats at concentrations of 0.005, 0.2, 10 and 200 mg/kg b.w. for 6 weeks. The overall body and organ weights were recorded. Serum levels of immunoglobulins, haematological values, histopathological lesions in different organs, and gene expression profiles in pituitary glands were investigated. The mean platelet volume was increased, and the monocyte count was decreased significantly in both male and female rats treated with tylosin. Compared to the untreated control, alanine transaminase in both types of rats and total serum bilirubin in female rats were increased significantly with the administration of tylosin (200 mg/kg), however, lactate dehydrogenase in female rats was decreased. The levels of immunoglobulin M were reduced in both male and female rats but immunoglobulin G levels were significantly reduced only in female rats which were treated with tylosin. Cell proliferation- and adhesion-associated genes were expressed more but apoptosis gene expressions were decreased in the pituitary gland of tylosin-treated rats. In conclusion, this study revealed that the use of tylosin at therapeutic dosage is possibly not completely safe.

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Section: Discussionsupporting

confidence: 80%

Physiological influence of tylosin tartrate overdose in vivo in rats

et al. 2020

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“…Periodontal ligament (PDL), the most important tissue influencing the orthodontic tooth movement, is composed of heterogeneous multipotent cell populations, which has the ability to undergo osteoblast differentiation [1]. Osteoblast differentiation of periodontal ligament cells (PDLCs) results in periodontal tissue remodeling and regenerating therefore it plays a vital role in maintaining homeostasis of periodontium.…”

Section: Introductionmentioning

confidence: 99%

miRNA-125b Regulates Osteogenic Differentiation of Periodontal Ligament Cells Through NKIRAS2/NF-κB Pathway

Nan¹,

Lin²,

Zhang³

et al. 2018

Cell Physiol Biochem

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Background/Aims: Osteogenesis of periodontal ligament cells (PDLCS) is essential for alveolar bone repair. Varieties of factors have been found involved in the regulation of PDLCs osteoblast differentiation. Aim of this study was to identify microRNA as a regulator of the os-teogenic differentiation of PDLCs. Methods: The CD markers were analyzed by flow cytometry analysis. Osteoblast differentiation of PDLCs was induced by treatment with dexamethasone, β-glycerol phosphate and α-ascorbic acid. The expression of osteoblastic phenotype was evaluated after the induction by simultaneous monitoring of alkaline phosphatase activity, the expression of genes involved in osteoblastic differentiation by RT-qPCR and Western Blot, and mineralization at the same time. MicroRNA and NKIRAS2 expression was determined by RT-qPCR. Luciferase reporter assays were performed to test whether miR-125b is capable of interacting with the 3’UTR sequence of NKIRAS2. The possible signaling pathway was determined by Western Blot. Results: In this study, we found that the expression of miR-125b was down regulated during the process of ostoblast differentiation of PDLCs. When the expression of miR-125b was up regulated, the osteogenic differentiation of PDLCs was inhibited. During this process, the over-expressed miR-125b led to the activation of NF-κB. NF-κB inhibitor interacting RAS-like 2 (NKIRAS2) is one of target gene of miR-125b, and it is a regulator of NF-κB signaling that plays various roles in osteoblastic differentiation. We demonstrate thatmiR-125b is involved in osteogenic differentiation of PDLCs. Conclusion: Our data support the hypothesis that that miR-125b attenuates PDLCs osteoblastic differentiation by targeting NKIRAS2 and enhancing NF-κB signaling.

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“…The most common are: blood flow pressure at the apex, collagen fibers of the periodontal ligament, root elongation, pulp and dentin proliferation, rate of alveolar bone remodeling, and presence of occlusal forces. 3–6 It is possible that one or more of these factors contribute to the eruption process and thus to discover the cellular and molecular actors in the post-emergent process, further research is required.…”

Section: Introductionmentioning

confidence: 99%

A longitudinal study on timing and velocity of rat molar eruption: Timing of rat molar eruption

et al. 2018

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Rat molar eruption and occlusion data were compiled from several studies but several inconsistencies were found, rendering the planning of eruptional studies difficult and imprecise. Our aim was to measure eruption and occlusion days, as well as eruption velocity, in the upper and lower three molars from infancy to end of adolescence in the rat. A total of 19 male and female Wistar rats were scanned daily by micro-computed tomography (CT) from day 15 to 70. We measured the eruption of all maxillary and mandibular molars with reference points at the hard palate and mandibular canal at three stages: pre-emergent, pre-occlusal, and functional. Statistical analysis was performed with a mixed-model analysis of variance (ANOVA) and a Sidak post hoc test. The first molar erupts on average on day 17, the second molar on day 20, and the third molar on day 33. The eruption velocity of the first molar was the highest at 90.9 microns/day (standard error (se) = 12.80), followed by the second molar at 65.9 microns/day (se = 5.80), and the lowest was the third at 47.0 microns/day (se = 3.28), ( p < 0.001). On average, the pre-occlusal phase had the highest velocity at 97.2 microns/day (se = 1.72), the pre-emergent was lower at 84.9 (se = 2.29), and the functional was the lowest at 21.7 (se = 0.45), ( p < 0.001). The eruption rate decreased from the first to third molar and was also different between phases: the pre-occlusal phase had the highest rate, closely followed by the pre-emergent phase while the functional eruption rate was significantly lower than the other phases.

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Increased Cell Proliferation and Gene Expression of Genes Related to Bone Remodeling, Cell Adhesion and Collagen Metabolism in the Periodontal Ligament of Unopposed Molars in Growing Rats

Cited by 8 publications

References 38 publications

Physiological influence of tylosin tartrate overdose in vivo in rats

Physiological influence of tylosin tartrate overdose in vivo in rats

miRNA-125b Regulates Osteogenic Differentiation of Periodontal Ligament Cells Through NKIRAS2/NF-κB Pathway

A longitudinal study on timing and velocity of rat molar eruption: Timing of rat molar eruption

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