Increased cell viability and proliferation in post-hypoxic hippocampal tissue culture treated with<em> Acalypha indica</em> root extract

Abstract

Abstrak (176,95%, 220,62%, 386,02% vs. 100%). Proliferasi sel pada kultur jaringan hipokampus tikus pascahipoksia dengan pemberian ekstrak akar kucing pada dosis 10, 15, dan 20 mg/mL lebih tinggi secara bermakna dibandingkan dengan kontrol (0,132; 0,117; 0,114 vs. 0,096). AbstractBackground: This research was done to study the infl uence of Acalypha indica Linn root extract towards relative cell viability and proliferation as parameters of neurogenesis in post-hypoxic hippocampal tissue culture. Methods Experimental in vitro study using 24 primary neuronal cell cultures obtained from adult Sprague Dawley rat exposed to hypoxia with 5% O2/5% CO2/N2 balance gas for 24 hours. Post-hypoxia, Acalypha indica Linn root extract was added at doses of 10, 15, and 20 mg/mL to 3 treatment groups. No treatment was given to the control group. Each group consists of 6 samples. After 90 hours of incubation, relative cell viability was measured by using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) examination, and cell proliferation was measured by using 5-bromo2'-deoxy-uridine (BrdU) for cell proliferation. Data was analyzed using one way ANOVA parametric tests, then further analyzed with post-hoc analysis. Results: The relative cell viability of rat hippocampal tissue culture treated with Acalypha indica Linn root extract with dose of 10, 15, and 20 mg/mL was signifi cantly higher than control (176.95%, 220.62%, and 386.02% vs. 100%). Cell proliferation of rat hippocampal tissue culture treated with Acalypha indica Linn root extract with dose of 10, 15, and 20 mg/mL was signifi cantly higher than control (0.132, 0.117, 0.114 vs 0.096). Conclusion: Acalypha indica

<em>Acalypha indica</em> root extract increases post-hypoxic rat hippocampal tissue culture cell viability via phospholipase A₂ inhibition

“…MTT test results were in accordance with the author's previous study, 18 indicating that Acalypha indica Linn root extract has an effect on increasing the number of viable post-hypoxic nerve cells with an optimum dose at 10 mg/mL. The mechanism of the increased cell proliferation is thought to be due to the decrease in PLA 2 enzyme activity by acalyphin which will in turn decrease the inflammatory response and mobilize the endogenous stem cells to proliferate.…”

“…Results of relative cell viability were in accordance with our previous study. 18 Relative mean PLA 2 enzyme level in the control group was 7.96 ± 0.060 ng/mL (n = 6). In groups treated with Acalypha indica Linn extract at doses of 10, 15, and 20 mg/mL, mean PLA 2 enzyme level was 5.55 ± 0.970 ng/mL (n=6), 6.85 ± 0.590 ng/mL (n = 6), 7.42 ± 0.090 ng/mL (n = 6), respectively (p = 0.04 in all treatment groups vs control; Figure 1).…”

“…16 The plants have many active chemical compounds; some that have been identified are kaempferol (flavonoids), beta-sitosterol, HCN, gamma-sitosterol, and acalyphin. 17 Yolanda, et al 18 have studied Acalypha indica Linn's neurotherapeutic effect on post-hypoxic nerve cells. Nirmal, et al 19 have proven that acalyphin and stigmasterol exert their anti-inflammatory effects by forming PLA 2 inhibitor complexes.…”

Acalypha Indica: Pemanfaatan dan Bioaktivitasnya

“…Proliferasi sel pada kultur jaringan hipokampus tikus pasca hipoksia dengan pemberian ekstrak akar kucing pada dosis 10, 15, dan 20 mg/mL lebih tinggi secara bermakna dibandingkan dengan kontrol (0,132; 0,117; 0,114 vs. 0,096). Ekstrak AI dapat meningkatkan viabilitas relatif dan proliferasi sel pascahipoksia in vitro pada dosis 10, 15, dan 20 mg/mL (Yolanda et al 2011).…”

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