Increased Cellular Proliferation in Rat Skeletal Muscle and Tendon in Response to Exercise: Use of FLT and PET/CT

Skovgaard, Dorthe; Bayer, Monika L.; Mackey, Abigail L.; Madsen, Jacob; Kjær, Michael; Kjær, Andreas

doi:10.1007/s11307-010-0316-y

Cited by 9 publications

(7 citation statements)

References 34 publications

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“…The exercise protocol in the present study was similar to an earlier study where PET and the tracer 3′-deoxy-3′-[ 18 F]fluoro-thymidine (FLT) were used for detecting an increased cellular proliferation in Achilles tendon and skeletal muscle [39] . Therefore, a parallel increased production of extracellular matrix proteins like the collagens was obviously expected as part of the adaptation process.…”

Section: Discussionmentioning

confidence: 99%

Use of Cis-[18F]Fluoro-Proline for Assessment of Exercise-Related Collagen Synthesis in Musculoskeletal Connective Tissue

et al. 2011

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Protein turnover in collagen rich tissue is influenced by exercise, but can only with difficulty be studied in vivo due to use of invasive procedure. The present study was done to investigate the possibility of applying the PET-tracer, cis-[18F]fluoro-proline (cis-Fpro), for non-invasive assessment of collagen synthesis in rat musculoskeletal tissues at rest and following short-term (3 days) treadmill running. Musculoskeletal collagen synthesis was studied in rats at rest and 24 h post-exercise. At each session, rats were PET scanned at two time points following injection of cis-FPro: (60 and 240 min p.i). SUV were calculated for Achilles tendon, calf muscle and tibial bone. The PET-derived results were compared to mRNA expression of collagen type I and III. Tibial bone had the highest SUV that increased significantly (p<0.001) from the early (60 min) to the late (240 min) PET scan, while SUV in tendon and muscle decreased (p<0.001). Exercise had no influence on SUV, which was contradicted by an increased gene expression of collagen type I and III in muscle and tendon. The clearly, visible uptake of cis-Fpro in the collagen-rich musculoskeletal tissues is promising for multi-tissue studies in vivo. The tissue-specific differences with the highest basal uptake in bone are in accordance with earlier studies relying on tissue incorporation of isotopic-labelled proline. A possible explanation of the failure to demonstrate enhanced collagen synthesis following exercise, despite augmented collagen type I and III transcription, is that SUV calculations are not sensitive enough to detect minor changes in collagen synthesis. Further studies including kinetic compartment modeling must be performed to establish whether cis-Fpro can be used for non-invasive in-vivo assessment of exercise-induced changes in musculoskeletal collagen synthesis.

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Section: Discussionmentioning

confidence: 99%

Use of Cis-[18F]Fluoro-Proline for Assessment of Exercise-Related Collagen Synthesis in Musculoskeletal Connective Tissue

et al. 2011

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“…It has been reported that treadmill running enhances mouse patellar and Achilles tendon stem/progenitor cell proliferation and increases collagen production (70% and 200% respectively) (33). Enhanced cellular proliferation in rat skeletal muscle and tendon was reported by Skovgaard et al (42) as a result of only 3 d of endurance training. Furthermore, repeated stretching exercises of rat calcaneal tendon (10 repetitions three or five times a week for 21 d) induced cellularity and collagen increase, and about twice the amount of glycosaminoglycan synthesis (43).…”

Section: Introductionmentioning

confidence: 90%

Role of moderate exercising on Achilles tendon collagen crimping patterns and proteoglycans

Franchi

Torricelli²,

Giavaresi³

et al. 2013

Connective Tissue Research

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In this study, the morphological and morphometric changes in the collagen crimping pattern of Achilles tendon and metabolism/expression of tenocytes explanted from tendons of running (RUN) and sedentary (SED) rats were investigated to assess the effects of 12 weeks moderate running exercise. The number, the top angle width and the base length of each crimp in three different regions (proximal, central and distal) of RUN and SED tendons were measured with a polarized light microscope. The most significant morphometric differences in the crimps were detectable in the central region of the RUN tendons. In this region, crimps were fewer, larger and more flattened than those of other regions as a consequence of a functional adaptation of extracellular matrix to running, in order to increase tendon stiffness and force transmission efficiency. Conversely, the top angle width of the crimps reduced in proximal and distal regions of the RUN tendons, suggesting that these crimps might act as more reactive mechanical springs, able to store and improve the release of the stored strain energy in most loaded regions. Tenocytes explanted from Achilles tendons of both RUN and SED groups were cultured. Running influenced tenocytes which showed a significant increase in collagen type-I synthesis and proteoglycans production, suggesting enhancement of the loading transmission efficiency and facilitate inter-fibril and inter-fiber sliding.

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“…In addition to the functional aspect of scintigraphy, PET offers additional advantages such as cross‐sectional imaging, higher sensitivity, and spatial resolution, and the availability of a variety of tracers . In particular, the use of 18 F‐FDG PET could improve detection of inflammation compared to using radiolabeled white blood cells, and could also improve investigation of tendinopathy/desmopathy in the horse …”

Section: Introductionmentioning

confidence: 99%

Positron Emission Tomography of the Equine Distal Limb: Exploratory Study

Spriet

Espinosa

Kyme

et al. 2016

Vet Radiology Ultrasound

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Positron emission tomography (PET) is a highly sensitive, noninvasive imaging technique for quantifying biological functions of tissues. However, at the time of this study, PET imaging applications had not been reported in the horse. The aim of this exploratory study was to determine whether a portable high-resolution PET scanner could be used to image the equine distal limb. Images of the front feet and fetlocks of three research horses, with known lesions localized to the distal front limbs, were acquired under general anesthesia after administration of F-fluorodeoxyglucose ( F-FDG), with doses ranging from 1.5 to 2.9 MBq/kg. The radiation exposure measured during imaging was slightly higher than Technetium scintigraphy. However, the use of general anesthesia allowed the proximity and the contact time with the patient to be minimized for the staff involved. F-FDG uptake was evident throughout the soft tissues, with the highest uptake in the coronary band and the lowest uptake in the tendons. Anatomic structures could be discriminated due to the high contrast between soft tissue and bone. Detected lesions included lysis of the flexor cortex of the navicular bone, lesions of flexor tendons and suspensory ligament, and abnormal uptake through the lamina of a laminitic subject. Findings indicated that tomographic molecular imaging is feasible in the equine distal limb and could be useful as a future diagnostic technique for clinical and research studies, especially those involving tendinopathy/desmopathy and laminitis.

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Increased Cellular Proliferation in Rat Skeletal Muscle and Tendon in Response to Exercise: Use of FLT and PET/CT

Abstract: FLT-PET seems to be a promising tool for imaging of exercise-induced cellular proliferation in musculo-tendinous tissue.

Cited by 9 publications

References 34 publications

Use of Cis-[18F]Fluoro-Proline for Assessment of Exercise-Related Collagen Synthesis in Musculoskeletal Connective Tissue

Use of Cis-[18F]Fluoro-Proline for Assessment of Exercise-Related Collagen Synthesis in Musculoskeletal Connective Tissue

Role of moderate exercising on Achilles tendon collagen crimping patterns and proteoglycans

Positron Emission Tomography of the Equine Distal Limb: Exploratory Study

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