Increased circulating hematopoietic and endothelial progenitor cells in the early phase of acute myocardial infarction

Abstract

Endothelial progenitor cell (EPC) mobilization has been reported following tissue damage, whereas no data are available regarding the mobilization of hematopoietic progenitor cells (HPCs). We performed the phenotypic and functional analysis of circulating CD34 ؉ progenitor cells in patients with acute myocardial infarction (AMI), assessed from admission up to 60 days, in patients with stable angina pectoris (SA), and in healthy controls (CTRLs). In patients with AMI at admission (T0), the number of circulating CD34 ؉ cells was higher (P < .001) than in CTRLs and became comparable with CTRLs within 60 days. Both the number of CD34 ؉ cells coexpressing CD33, CD38, or CD117 and the number of HPCs was higher (P < .02 for all) in patients with AMI at T0 than in CTRLs, as was the number of hematopoietic colonies (P < .

Level of CD14+-endothelial progenitor cells is not associated with coronary artery disease or cardiovascular risk factors

“…These two groups came to similar conclusions by diverse experimental methodology, one group culturing mononuclear cells initially adherent to fibronectin and another group discarding the adherent cells and culturing those initially nonadherent. Massa et al (2005) observed no significant difference in the number of circulating CD34 + KDR + cells in patients with stable CAD compared with healthy controls. Güven et al(2006) reported that the number of EPC colonies was increased in proportion with the severity of CAD.…”

“…George et al ( 2004) found higher number of EPC colonies in patients with UA as compared with stable angina. Massa et al (2005) showed that the number of CD34 + KDR + cells in the blood was increased in patients with acute MI compared with healthy controls or patients with stable CAD. Schmidt-Lucke et al (2005) detected a significant decreased level of CD34 + KDR + cells in patients with ACS compared with healthy controls.…”

“…It follows that the number of circulating EPCs may reflect the "vascular health" of an individual, and thus represent a marker by which to assess cardiovascular disease risk. However, the lack of a uniform EPC definition complicates cross-study comparisons and may contribute to the apparent paradox of some studies that suggest that EPCs are reduced in the presence of cardiovascular risk factors and coronary artery disease (CAD) (Vasa et al 2001;Hill et al 2003;Schmidt-Lucke et al 2005), whereas others suggest that numbers are increased in those with obstructive CAD (George et al 2004;Massa et al 2005;Güven et al 2006).…”

“…More importantly, there are data indicating that the vast majority of EPCs actually arise from the CD14 + subpopulation of peripheral blood mononuclear cells (PBMCs) (Rehman et al 2003;Gulati et al 2003;Romagnani et al 2005). However, previous studies focusing on the relationship between either CAD or cardiovascular risk factors and the level of EPCs either did not distinguish between the two types of EPCs (Vasa et al 2001;Hill et al 2003;George et al 2004) or studied only CD34 + -EPCs (Schmidt-Lucke et al 2005;Massa et al 2005). Therefore, the question of whether the level of CD14 + -EPCs correlates with either CAD or cardiovascular risks remains unresolved.…”

Relationship between stem cell factor/c-kit expression in peripheral blood and blood pressure

“…Once the endothelium is activated, stem cells, such as mesenchymal stem cells, endothelial progenitor cells and peripheral blood stem cells, are mobilized to promote vascular repair or angiogenesis. 2 Massa et al 3 showed that CD34 þ peripheral blood stem cells and endothelial progenitor cells are significantly elevated in patients with acute myocardial infarction and remain elevated for 2 months after the event. Another report showed that an alteration in the pro-angiogenic function of progenitor cells may contribute to hypertension-induced impairment of post-ischaemic revascularization.…”