Increased circulating platelet–neutrophil, platelet–monocyte complexes, and platelet activation in patients with ulcerative colitis: A comparative study

Abstract

It is reported that the incidence of thromboembolism is increased in ulcerative colitis (UC), and hypercoagulability persists even when patients are in remission. We evaluated the association of inflammatory response parameters with UC activity, and activation parameters of the platelets, endothelium, and the coagulation system in UC. Eighteen UC patients and 19 healthy subjects were included in the study. The patients' clinical features were recorded down; whole blood counts and acute phase parameters were evaluated. UC patients were divided into two as active (9 patients) and inactive (9 patients) according to combined clinical activity index (CAI) and endoscopic activity index (EAI) scores. In all subjects, platelet CD62P expression, platelet-monocyte complexes (PMC), platelet-neutrophil complexes (PNC), and platelet microparticles (PMP) were determined by flow cytometry. E-selectin, thrombin-antithrombin complex (TAT) levels in plasma, and sCD40L levels in serum were determined by ELISA. In both active and inactive UC patients, platelet CD62P expression, the percentages of PMC, and PNC were significantly higher than those in the control group (P < 0.01). PMP level was higher in the control group than in inactive UC patients (P = 0.001). sCD40L level was significantly higher in active UC group than in the control group (P < 0.01). EAI score correlated significantly with PMP (r = 0.5, P = 0.04) and sCD40L (r = 0.48, P = 0.044); CAI score had a negative correlation (r = -0.68, P = 0.002) with sE-selectin level. In addition to increased CD62P expression and sCD40L, increased formation of PMC and PNC suggests a role for plateletleukocyte complex formation together with platelet activation in thromboembolic events observed in UC. Am. J. Hematol. 81:753-759, 2006. V V C 2006 Wiley-Liss, Inc.

Microparticles: new light shed on the understanding of venous thromboembolism

“…Despite circulating in both blood and biological fluids in healthy individuals, MPs have been implicated in a wide spectrum of diseases, including cardiovascular disease [2][3][4] , diabetes [5] , inflammatory states [6,7] , sepsis [8] , antiphospholipid antibody syndrome [9,10] , thrombotic thrombocytopenic purpura [11] and cancer [12] . Many of these conditions are characterized by a hypercoagulable state, which may lead to thrombosis.…”

Assessment of the influence of the inflammatory process on the activation of blood platelets and morphological parameters in patients with ulcerative colitis (<i>colitis ulcerosa</i>)

“…Previous studies have shown that there is an increase in the concentration of IL-6 in ulcerative colitis and its role is to initiate, potentiate and sustain the inflammatory process in the inflamed mucous membrane of the colon [8][9][10]. Cytokines and interactions between blood cells cause the activation of blood platelets [1,11].…”

Increased levels of circulating platelet derived microparticles in Crohn's disease patients

“…Palkovits et al [15] found increased PDMPs levels only in patients with quiescent IBD compared to controls, but no difference in PDMPs levels was reported when active and quiescent patient groups were compared. Pamuk et al [16] failed to show any increase in PDMPs levels in UC patients and they even reported increased PDMPs levels in healthy controls compared to inactive UC patients. Finally, Leonetti et al [17] displayed increased PDMPs only in active CD patients compared to healthy controls, while a UC population was absent.…”

“…Previous studies revealed overall elevated PDMPs levels in IBD compared to healthy controls (Table 3 summarizes data from available studies). [13][14][15][16][17] However, to the best of our knowledge, unanswered remains the question as to whether established IBD medication treatments pose any impact on PDMPs levels. Our work illustrates that (i) there is significant increase of studied PDMPs levels, including those bearing exposed phosphatidylserine in CD patients compared to healthy individuals.…”

“…These observations have prompted to investigation of PDMPs in IBD [13][14][15][16][17] with major inconsistencies among their results, mainly due to differences in the studied populations and in the methodical approaches applied. To this end, we conducted a study aiming to evaluate the levels of circulating PDMPs and PDMPs expressing Annexin V in peripheral blood of IBD patients using flow cytometry and to compare them to those of healthy individuals.…”