Increased DNA-Dependent RNA Polymerase Activity from CV-1 Cells Productively Infected by SV40

Abstract

Two forms of DNA-dependent RNA polymerase were isolated from CV-1 cell cultures that are permissive to infection by SV40. RNA polymerases I and II were partially purified by chromatographic fractionation on DEAE-cellulose columns and had the same properties as analogous enzymes recovered from other eukaryotic cells. SV40 infection stimulated activity of both enzymes. The major increase in enzyme activity occurred with RNA polymerase II and was detectable before virion production.