Increased Expression of a Myc Target Gene Mina53 in Human Colon Cancer

Teye, Kwesi; Tsuneoka, Makoto; Arima, Nobuyuki; Koda, Yoshiro; Nakamura, Yasuhiro; Ueta, Yoichi; Shirouzu, Kazuo; Kimurâ, Hiroshi

doi:10.1016/s0002-9440(10)63111-2

Cited by 68 publications

(98 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Expression of mina53 is increased during cell proliferation, and specific inhibition of mina53 expression by an RNA interference method suppressed cell proliferation in some cultured cell lines (25,26). Recently, we generated a specific monoclonal antibody against human Mina53 protein and found that expression of Mina53 is frequently increased in human colon cancer (26). Here we show that Mina53 is highly expressed in most ESCC cells and that its expression level in tumors relates to the outcome of patients with ESCC.…”

Section: Introductionmentioning

confidence: 65%

“…One hybridoma secreting an IgG2a antibody, clone M532, was used in this study. The specificity of M532 to human Mina53 was demonstrated in our previous study (26). The antibody was produced as ascitic fluid in prestane-preinjected mice and purified.…”

Section: Methodsmentioning

confidence: 78%

“…We established hybridoma clones secreting anti-human Mina53 antibodies (26). One hybridoma secreting an IgG2a antibody, clone M532, was used in this study.…”

Section: Methodsmentioning

confidence: 99%

“…One tissue block containing a marginal region of tumor was selected from each patient. Immunostaining of tissues was performed essentially as described previously (26). In brief, thin sections of formalin-fixed paraffinembedded tissue specimens were mounted on glass slides, deparaffinized, and autoclaved for 20 minutes in 10 mmol/L sodium citrate buffer (pH 6.0) for antigen retrieval.…”

Section: Methodsmentioning

confidence: 99%

“…The mina53 gene encodes a protein with a molecular weight of 53,000 that is localized in the nucleus, with part of the protein concentrated in the nucleolus. Expression of mina53 is increased during cell proliferation, and specific inhibition of mina53 expression by an RNA interference method suppressed cell proliferation in some cultured cell lines (25,26). Recently, we generated a specific monoclonal antibody against human Mina53 protein and found that expression of Mina53 is frequently increased in human colon cancer (26).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Mina53 as a Potential Prognostic Factor for Esophageal Squamous Cell Carcinoma

Tsuneoka

Fujita

Arima

et al. 2004

Clinical Cancer Research

Self Cite

View full text Add to dashboard Cite

Purpose: We previously identified mina53, a novel Myc target gene. Here we investigated whether mina53 is related to esophageal squamous cell carcinoma (ESCC), a disease with poor prognosis.Experimental Design: Mina53 expression was suppressed in ESCC cell lines by a RNA interference method to investigate whether Mina53 is involved in cell proliferation. Expression of Mina53 was investigated by Western blotting in tissue sections from patients with ESCC. Immunohistochemical analysis of Mina53 was carried out and compared with that using anti-Ki-67 antibody. Finally, the level of Mina53 expression was compared with the length of survival of patients with ESCC.Results: Reduction of mina53 expression by RNA interference suppressed cell proliferation in ESCC cell lines. Western blot analysis of surgically resected ESCC specimens indicated that the expression of Mina53 in tumors was increased compared with that in adjacent nonneoplastic tissues in all four specimens examined. When formalin-fixed specimens from 52 patients with ESCC were stained immunohistochemically, it was found that Mina53 was highly expressed in 83% of specimens. Anti-Mina53 antibody stained tumors more efficiently than antibody against Ki-67, a cell proliferation biomarker, in some cancer specimens. Patients with high expression of Mina53 had shorter survival periods, whereas the expression level of Ki-67 in ESCC showed no relationship to patient outcome. Conclusions:Taken together, our results indicate that expression of Mina53 is a characteristic feature of ESCC and suggest that immunostaining by anti-Mina53 antibody may be useful as a potential prognostic indicator.

show abstract

Section: Introductionmentioning

confidence: 65%

Section: Methodsmentioning

confidence: 78%

“…We established hybridoma clones secreting anti-human Mina53 antibodies (26). One hybridoma secreting an IgG2a antibody, clone M532, was used in this study.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Mina53 as a Potential Prognostic Factor for Esophageal Squamous Cell Carcinoma

Tsuneoka

Fujita

Arima

et al. 2004

Clinical Cancer Research

Self Cite

View full text Add to dashboard Cite

show abstract

MDIG in Breast Cancer Progression and Metastasis

Thakur,

Qiu,

et al. 2024

Advances in Experimental Medicine and Biology

View full text Add to dashboard Cite

Hydroxylation and translational adaptation to stress: some answers lie beyond the STOP codon

Katz

Gándara

Ezcurra

et al. 2016

Cell. Mol. Life Sci.

View full text Add to dashboard Cite

Regulation of protein synthesis contributes to maintenance of homeostasis and adaptation to environmental changes. mRNA translation is controlled at various levels including initiation, elongation and termination, through post-transcriptional/translational modifications of components of the protein synthesis machinery. Recently, protein and RNA hydroxylation have emerged as important enzymatic modifications of tRNAs, elongation and termination factors, as well as ribosomal proteins. These modifications enable a correct STOP codon recognition, ensuring translational fidelity. Recent studies are starting to show that STOP codon read-through is related to the ability of the cell to cope with different types of stress, such as oxidative and chemical insults, while correlations between defects in hydroxylation of protein synthesis components and STOP codon read-through are beginning to emerge. In this review we will discuss our current knowledge of protein synthesis regulation through hydroxylation of components of the translation machinery, with special focus on STOP codon recognition. We speculate on the possibility that programmed STOP codon read-through, modulated by hydroxylation of components of the protein synthesis machinery, is part of a concerted cellular response to stress.

show abstract

Increased Expression of a Myc Target Gene Mina53 in Human Colon Cancer

Cited by 68 publications

References 39 publications

Mina53 as a Potential Prognostic Factor for Esophageal Squamous Cell Carcinoma

Mina53 as a Potential Prognostic Factor for Esophageal Squamous Cell Carcinoma

MDIG in Breast Cancer Progression and Metastasis

Hydroxylation and translational adaptation to stress: some answers lie beyond the STOP codon

Contact Info

Product

Resources

About