Increased expression of glycodelin mRNA and protein in rat lungs during ovalbumin-induced allergic airway inflammation

Abstract

Asthma is a chronic inflammatory disease accompanied by airway obstruction and hyper-responsiveness. Asthmatic inflammation is characterized by the expression of multiple genes for inflammatory mediators. Glycodelin is a glycoprotein with several functions in cell recognition and differentiation. There is substantial evidence that glycodelin may be a mediator for immunomodulatory and immunosuppressive effects on several human tissues. To determine the potential role of glycodelin in the pulmonary immune response, we examined the distribution of the glycodelin mRNA and protein in an experimental rat model of allergen-induced airway inflammation. The experimental model developed an airway response to inhaled nebulized ovalbumin in adult rats. Two groups of rats (ovalbumin and saline) were challenged for 3 weeks, lungs were fixed and embedded, and sections were studied for expression of glycodelin mRNA by in situ hybridization and protein by immunohistochemistry. Glycodelin is expressed in Clara cells of bronchial epithelium, type II pneumocytes and alveolar macrophages. Densitometric analyses show a significant increase of the glycodelin mRNA and protein expression in rat lungs after ovalbumin challenge. Induced glycodelin amounts in tissue, particularly in Clara cells and alveolar macrophages were found. The altered expression pattern of glycodelin may contribute to the pulmonary immune response in asthmatic inflammation.

Identification of stage-specific biomarkers in lung adenocarcinoma based on RNA-seq data

“…It plays key roles in modulating B cell responses [22], regulating cytokine production in natural killer cells [23], inducing tolerance of dendritic cells [24], T cells apoptosis [25], and Th-2 shift in cytokines [26,27]. PAEP is expressed in type II pneumocytes, bronchial epithelium cells, and alveolar macrophages which are involved in the pulmonary immune response in the process of asthmatic inflammation [28,29]. Since over-expression of PAEP in the late stage of LAC, it may accelerate LAC progression through immunosuppression.…”

Immunosuppressive Glycodelin A is an independent marker for poor prognosis in endometrial cancer

“…Non-radioactive in situ-hybridization (ISH) analysis of Gd was performed on paraffin sections as described previously [27,28,34]. Briefly, paraffin sections were deparaffined, rehydrated and permeabilized by pepsin digestion (750 mg/ml pepsin in 0.2 M HCl, 37C, 30 min).…”

“…Bound riboprobe was visualized by incubation with alkaline phosphatase-conjugated anti-DIG antibody (Roche Biochemicals) and subsequent substrate reaction using 5-bromo-4-chloro-3-indolyl phosphate/nitroblue-tetrazolium chloride [27,28,34]. …”

“…Gd mRNA expression was analysed automatically in a computer aided procedure as published previously by our group [27,28,34]. Briefly, five digital pictures were taken randomly from each tissue section (3CCD color camera, HV-C20M, Hitachi, Denshi, Japan, and Axiolab, Carl Zeiss, Jena, Germany).…”

β-Lactoglobulin and Glycodelin: Two Sides of the Same Coin?

“…It is in fact the sequence of βLg-B. In situ hybridization on rat genital tract and PCR followed by sequencing has identified 100 bases of mRNA sharing “100% homology” with human glycodelin ( Keil et al, 1999 ) and polyclonal antibodies to human Gd cross-react with rat reproductive and lung tissues ( Kunert-Keil et al, 2005 , 2009 ; Erdil et al, 2020 ). While false positive results arising in antibody cross reactivity experiments are not uncommon, the apparent presence of Gd in the rat by mRNA hybridization ( Keil et al, 1999 ) remains something of a mystery.…”