Increased expression of long intergenic non-coding RNA LINC00152 in gastric cancer and its clinical significance

Abstract

It has been known that differential expression of long non-coding RNA (lncRNA) plays critical roles in carcinogenesis. However, the significance of lncRNA, especially long intergenic ncRNA (lincRNA, the main type of lncRNA family), in the diagnosis of gastric cancer is largely unknown. The aim of this study was to determine the expression level of LINC00152, a newfound lincRNA, in gastric carcinoma and its clinical association. The expression of LINC00152 in 71 pairs of tumorous and adjacent normal tissues from patients with gastric cancer was detected by quantitative real-time reverse transcription-polymerase chain reaction. And then, the potential associations between its level in gastric cancer tissue and the clinicopathological features were analyzed. Finally, a receiver operating characteristic (ROC) curve was constructed for differentiating patients with gastric cancer from patients with benign gastric diseases. The results showed that the expression level of LINC00152 in gastric carcinoma was significantly increased, compared with matched normal tissue (P=0.045) and normal mucosa from health control (P=0.004), respectively. Levels of LINC00152 in gastric cancer cell lines, BGC-823, MGC-803, and SGC-7901, were significantly higher than those in human normal gastric epithelial cell line GES-1. In addition, high expression of LINC00152 was correlated with invasion (P=0.042). LINC00152 levels in gastric juice from patients with gastric cancer were further found significantly higher than those from normal controls (P=0.002). Moreover, the area under the ROC curve (AUC) was up to 0.645 (95 % CI=0.559-0.740, P=0.003). This study highlights that lincRNA LINC00152 might be a novel biomarker for predicting gastric cancer.

HULC and Linc00152 Act as Novel Biomarkers in Predicting Diagnosis of Hepatocellular Carcinoma

“…For the purpose of indentifying plasma lncRNAs as potential biomarkers, we searched candidate databases of lncRNAdb, LNCipedia, and LncRNA Disease. Previous studies showed that UCA1, TUG1, CCAT1 and Linc00152 were up-regulated obviously in tumor tissues compared with nontumor tissues [16][17][18][19], while MEG3 was down-regulated significantly in tumor tissues. Regulations of all these lncRNAs in HCC have not been clarified yet.…”

Short-lived non-coding transcripts (SLiTs): Clues to regulatory long non-coding RNA

“…First, LINC00152 expression is increased in gastric cancer tissue (23). The expression level of LINC00152 in gastric carcinoma is significantly increased compared with matched normal tissue and normal mucosa from healthy controls.…”

Roles of long noncoding RNAs in gastric cancer and their clinical applications

“…It was demonstrated that LINC00152, gastric cancerassociated transcript 2 (GACAT2, AC130710), small ubiquitin-like modifier (SUMO) 1 pseudogene 3 (SUMO1P3), and ABHD11 antisense RNA 1 (ABHD11-AS1) were markedly up-regulated in gastric cancer tissues (Lin et al 2014;Mei et al 2013;Pang et al 2014;Xu et al 2014), while GACAT1 (AC096655.1-002), GACAT2 (HMlincRNA717), AA174084, FER1L4, and AI364715 were significantly down-regulated in gastric cancer tissues Shao et al 2014a, b;Sun et al 2013;Zhu et al 2015). More important, as AC096655.1-002, HMlincRNA717, and AC130710 were firstly found to be closely related to gastric cancer by us, they have been officially nominated as gastric cancer-associated transcript 1 (GACAT1), GACAT2, and GACAT3, respectively Xiao and Guo 2013).…”