Increased expression of nerve growth factor (NGF) and high affinity NGF receptor (p140 TrkA) in human osteoarthritic chondrocytes

Iannone, Florenzo; Bari, Cosimo De; Dell’Accio, Francesco; Covelli, M.; Patella, Vittorio; Bianco, Gianpatrizio; Lapadula, Giovanni

doi:10.1093/rheumatology/41.12.1413

Cited by 135 publications

(105 citation statements)

References 30 publications

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“…We assessed the microarray data for genes that in previous studies were shown to be differentially expressed in intact versus damaged regions of OA cartilage from the same joint or in normal versus OA cartilage from different patients (4)(5)(6)(7)14,(34)(35)(36)(37)(38). The name and symbol of the reported genes are shown in Table 3.…”

Section: Results Of Real-time Quantitative Pcr Of Selected 8 Genesmentioning

confidence: 99%

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Comparative analysis of gene expression profiles in intact and damaged regions of human osteoarthritic cartilage

Sato

Konomi

Yamasaki

et al. 2006

Arthritis & Rheumatism

154

130

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Objective. To analyze the differences in gene expression profiles of chondrocytes in intact and damaged regions of cartilage from the same knee joint of patients with osteoarthritis (OA) of the knee.Methods. We compared messenger RNA expression profiles in regions of intact and damaged cartilage (classified according to the Mankin scale) obtained from patients with knee OA. Five pairs of intact and damaged regions of OA cartilage were evaluated by oligonucleotide array analysis using a double in vitro transcription amplification technique. The microarray data were confirmed by real-time quantitative polymerase chain reaction (PCR) amplification and were compared with previously published data.Results. About 1,500 transcripts, which corresponded to 8% of the expressed transcripts, showed >2-fold differences in expression between the cartilage tissue pairs. Approximately 10% of these transcripts (n ‫؍‬ 151) were commonly expressed in the 5 patient samples. Accordingly, 114 genes (35 genes expressed in intact > damaged; 79 genes expressed in intact < damaged) were selected. The expression of some genes related to the wound-healing process, including cell proliferation and interstitial collagen synthesis, was higher in damaged regions than in intact regions, similar to the findings for genes that inhibit matrix degradation. Comparisons of the real-time quantitative PCR data with the previously reported data support the validity of our microarray data.Conclusion. Differences between intact and damaged regions of OA cartilage exhibited a similar pattern among the 5 patients examined, indicating the presence of common mechanisms that contribute to cartilage destruction. Elucidation of this mechanism is important for the development of effective treatments for OA.

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Section: Results Of Real-time Quantitative Pcr Of Selected 8 Genesmentioning

confidence: 99%

“…Therefore, changes in the gene expression patterns of chondrocytes in response to various exogenous stimuli could affect the integrity of articular cartilage. Based on this concept, many groups of investigators have reported that gene expression in certain molecules differ from region to region within a single OA joint (4)(5)(6)(7).…”

mentioning

confidence: 99%

Comparative analysis of gene expression profiles in intact and damaged regions of human osteoarthritic cartilage

Sato

Konomi

Yamasaki

et al. 2006

Arthritis & Rheumatism

154

130

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“…NGF receptors were also detected on activated murine CD4 T cells (but not on resting T cells) (43), human B lymphocytes (44), monocytes (45), skin and lung fibroblasts (46), keratinocytes (40), melanocytes (47), periarterial connective tissue cells (40), and human osteoarthritic chondrocytes (48).…”

Section: Discussionmentioning

confidence: 99%

Connective Tissue Growth Factor CCN2 Interacts with and Activates the Tyrosine Kinase Receptor TrkA

Wahab

Weston

Mason

2005

Journal of the American Society of Nephrology

133

118

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“…It is well-known that these substances have important trophic and survival-promoting effects for the nervous system [Levi-Montalcini, 1987;Linnarsson et al, 2000]. Recent evidence indicates that they also have such effects for chondrocytes [Iannone et al, 2002]. NTs are suggested to be important in the regulation of bone formation [Asaumi et al, 2000], in the healing of skin ulcers [Aloe, 2004] and in wound healing in diabetic mice [Muangman et al, 2004].…”

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confidence: 99%

Expression Patterns of Neurotrophins and Neurotrophin Receptors in Articular Chondrocytes and Inflammatory Infiltrates in Knee Joint Arthritis

Grimsholm¹,

Guo

et al. 2008

Cells Tissues Organs

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Background: It is likely that neurotrophins (NTs) are of great importance for the articular cartilage and the inflammation process in arthritis. Methods: The immunohistochemical expression of the NTs nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) and the associated receptors p75, TrkA and TrkB was examined in the knee joint of arthritic and healthy mice. Results: Immunoreactions for NGF and BDNF were detected in cells and nerve fiber varicosities in the inflammatory infiltrates of the synovial tissue of arthritic joints but not in synovial tissue of controls. p75-immunoreactive nerve fiber-like strands were detected in inflammatory infiltrates. Immunostaining for NGF, BDNF, p75, TrkA and TrkB was noted in articular chondrocytes. There was a statistically significant decrease in reactions for NGF (p < 0.001), TrkA (p = 0.001) and p75 (p < 0.001) in articular chondrocytes in joints exhibiting severe arthritis. Conclusion: The findings show that an NT system develops in inflammatory infiltrates of the synovial tissue. Furthermore, most interestingly, autocrine/paracrine effects appear to exist concerning NTs for the articular chondrocytes. The downregulated expression of NGF and NT receptors in articular chondrocytes in arthritis is a new aspect concerning the involvement of NTs in cartilage.

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Increased expression of nerve growth factor (NGF) and high affinity NGF receptor (p140 TrkA) in human osteoarthritic chondrocytes

Cited by 135 publications

References 30 publications

Comparative analysis of gene expression profiles in intact and damaged regions of human osteoarthritic cartilage

Comparative analysis of gene expression profiles in intact and damaged regions of human osteoarthritic cartilage

Connective Tissue Growth Factor CCN2 Interacts with and Activates the Tyrosine Kinase Receptor TrkA

Expression Patterns of Neurotrophins and Neurotrophin Receptors in Articular Chondrocytes and Inflammatory Infiltrates in Knee Joint Arthritis

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