Increased expression of T cell activation markers (CD25, CD26, CD40L and CD69) in atherectomy specimens of patients with unstable angina and acute myocardial infarction

Hosono, Mitsuharu; Boer, Onno J. de; Wal, Allard C. van der; Loos, Chris M. van der; Teeling, Peter; Piek, Jan J.; Ueda, Makiko; Becker, Anton E.

doi:10.1016/s0021-9150(03)00024-8

Cited by 88 publications

(62 citation statements)

References 27 publications

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“…While monocyte macrophages and macrophagederived foam cells constitute the major cellular component of early atherosclerotic lesions, T cells represent an important additional component. Activation of both cell types is observed at all stages of atherosclerosis and, in particular, at sites of plaque activation and rupture in patients with unstable angina and myocardial infarction (1)(2)(3)(4). Cell culture studies show that activated macrophages and T cells secrete factors that may destabilize plaques (5,6).…”

Section: Introductionmentioning

confidence: 99%

Disruption of TGF-β signaling in T cells accelerates atherosclerosis

Robertson¹,

Rudling²,

Zhou³

et al. 2003

J. Clin. Invest.

138

143

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Increasing evidence suggests that atherosclerosis is an inflammatory disease promoted by hypercholesterolemia. The role of adaptive immunity has been controversial, however. We hypothesized that proatherogenic T cells are controlled by immunoregulatory cytokines. Among them, TGF-β has been implied in atherosclerosis, but its mechanism of action remains unclear. We crossed atherosclerosisprone ApoE-knockout mice with transgenic mice carrying a dominant negative TGF-β receptor II in T cells. The ApoE-knockout mice with disrupted TGF-β signaling in T cells exhibited a sixfold increase in aortic lesion surface area, a threefold increase in aortic root lesion size, and a 125-fold increase in aortic IFN-γ mRNA when compared with age-matched ApoE-knockout littermates. When comparing size-matched lesions, those of mice with T cell-specific blockade of TGF-β signaling displayed increased T cells, activated macrophages, and reduced collagen, consistent with a more vulnerable phenotype. Ab's to oxidized LDL, circulating T cell cytokines, and spleen T cell activity were all increased in ApoE-knockout mice with dominant negative TGF-β receptors in T cells. Taken together, these results show that abrogation of TGF-β signaling in T cells increases atherosclerosis and suggest that TGF-β reduces atherosclerosis by dampening T cell activation. Inhibition of T cell activation may therefore represent a strategy for antiatherosclerotic therapy.This article was publised online in advance of the print addition. The date of publication is available from the JCI website, http://www.jci.org.

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Section: Introductionmentioning

confidence: 99%

Disruption of TGF-β signaling in T cells accelerates atherosclerosis

Robertson¹,

Rudling²,

Zhou³

et al. 2003

J. Clin. Invest.

138

143

View full text Add to dashboard Cite

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“…1 In patients with unstable angina and acute myocardial infarction, the numbers of recently activated T cells are significantly increased. 2,3 Furthermore, de Palma et al 4 showed that unstable but not stable coronary atherosclerotic lesions contain oligoclonal populations of T lymphocytes, which shows that antigen-driven activation and proliferation of effector T cells typically occur in unstable plaques.…”

mentioning

confidence: 99%

Selective Expansion of Influenza A Virus–Specific T Cells in Symptomatic Human Carotid Artery Atherosclerotic Plaques

Keller

Meer

Teeling

et al. 2008

Stroke

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Background and Purpose-Evidence is accumulating that infection with influenza A virus contributes to atherothrombotic disease. Vaccination against influenza decreases the risk of atherosclerotic syndromes, indicating that inflammatory mechanisms may be involved. We tested the hypothesis that influenza A virus-specific T cells contribute to atherosclerotic plaque inflammation, which mediates the onset of plaque rupture. Methods-T-cell cultures were generated from atherosclerotic segments and peripheral blood of 30 patients with symptomatic carotid artery disease. The response of plaque and peripheral blood T cells to influenza A virus was analyzed and expressed as a stimulation index (SI). Selective outgrowth of intraplaque influenza A-specific T cells was calculated by the ratio of plaque T cell SI and peripheral blood T cell SI for each patient. Accordingly, the patients were categorized as high-(SI ratio Ն5), intermediate-(5 ϽSI ratio Յ2), and non-(SI ratio Ͻ2) responders. The presence of influenza A virus in the vessel fragments was evaluated by reverse transcription-polymerase chain reaction. Results-High proliferative responses of plaque-derived T cells to influenza

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“…As such, directly labeled primary antibodies are commercially available mainly for fluorescent cell sorter system purposes, and many of those fluorochrome conjugates may also work in IHC. Applicability of this multistep double staining procedure has been valuable in several publications from our group (Naruko et al 1996;de Boer et al 1997;Hosono et al 2003). 6.…”

Section: The Journal Of Histochemistry and Cytochemistry Multiple Immunmentioning

confidence: 99%

Multiple Immunoenzyme Staining: Methods and Visualizations for the Observation With Spectral Imaging

Loos

2007

J Histochem Cytochem.

195

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Several staining concepts and color combinations exist to perform successful double immunoenzyme staining on human tissue specimens. Most of these concepts are based on differences between both primary antibodies: animal species, mouse Ig isotype or IgG subclasses, conjugates, or concentrations. Traditionally, double immunoenzyme staining has used chromogens selected to provide maximum color contrast when observed with the unaided eye. Unfortunately, visually good color combinations always include at least one diffuse chromogen, because of the paucity of appropriate chromogen colors. This situation is drastically changed with the use of spectral imaging, where multicolor microscopy can be unmixed in individual images based on their spectral characteristics. Spectral unmixing can be performed even up to quadruple immunoenzyme staining. This work contains practical suggestions for immunoenzyme double staining procedures for some frequently encountered primary antibody combinations: rabbit–mouse, goat–mouse, mouse–mouse, and rabbit–rabbit. The suggested protocols are all suitable for a classical red-brown color combination plus blue nuclear counterstain that is composed of peroxidase activity (diaminobenzidine tetrahydrochloride), alkaline phosphatase activity (Liquid Permanent Red), and hematoxylin, respectively. Although the red and brown chromogens do not contrast very well visually, they both show a crisp localization and can be perfectly unmixed by spectral imaging.

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Increased expression of T cell activation markers (CD25, CD26, CD40L and CD69) in atherectomy specimens of patients with unstable angina and acute myocardial infarction

Cited by 88 publications

References 27 publications

Disruption of TGF-β signaling in T cells accelerates atherosclerosis

Disruption of TGF-β signaling in T cells accelerates atherosclerosis

Selective Expansion of Influenza A Virus–Specific T Cells in Symptomatic Human Carotid Artery Atherosclerotic Plaques

Multiple Immunoenzyme Staining: Methods and Visualizations for the Observation With Spectral Imaging

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