Increased IL17A, IFNG, and FOXP3 Transcripts in Moderate-Severe Psoriasis: A Major Influence Exerted by IL17A in Disease Severity

Abstract

Psoriasis is a chronic and recurrent dermatitis, mediated by keratinocytes and T cells. Several proinflammatory cytokines contribute to formation and maintenance of psoriatic plaque. The Th1/Th17 pathways and some of IL-1 family members were involved in psoriasis pathogenesis and could contribute to disease activity. Therefore, we sought to analyse skin transcript levels of IL17A, IL22, RORC, IL8, IFNG, IL33, IL36A, FOXP3, and IL10 and correlate with clinic of patients with plaque-type psoriasis. In order to conduct that, we collected punch biopsies from lesional skin and obtained tissue RNA. After reverse transcription, qRT-PCR quantified the relative mRNA expression. The main results revealed increased transcripts levels of IL17A, IFNG, and FOXP3 in moderate-severe patients. Despite this, only IL17A can increase the chance to worsen disease severity. We also observed many significant positive correlations between each transcript. In conclusion, IL17A is elevated in lesional skin from psoriasis patients and plays crucial role in disease severity.

Levels of the interleukins 17A, 22, and 23 and the S100 protein family in the gingival crevicular fluid of psoriatic patients with or without periodontitis

“… 2 Evidence supporting this hypothesis includes the isolation of IL-23 and IL-17 mRNA from psoriatic skin lesions and the report of increased serum concentrations of IL-17A, IL-22, and IL-23 in psoriatic patients vs. healthy controls. 3 , 4 , 5 In recent years, special attention has been given to the prospective role of S100 proteins in the development of psoriasis. The S100 protein family is composed of calcium-binding proteins, in which psoriasin (S100A7) and calgranulin A (S100A8) are regulated by IL-17, amplifying the inflammatory response.…”

Psoriasis and multiple sclerosis – hidden link?

“…found a significant association with the progression‐related outcomes of MS with a negative correlation meaning that psoriasis induced a slower progression of disease in MS. Unfortunately, the authors did not evaluate the IL‐17A levels which are known to correlate best with the severity of psoriasis . So coming from single case presentation with a correlation of the both disease and some ideas about the hidden Links to the epidemiological statistics in cohort studies and large population studies, there are more and more evidence of the background comorbidities and possible immunological links between both diseases, although there are some doubts about the association …”