Increased Osteogenesis Elicited by Boron-Modified Bioactive Glass Particles in the SiO₂-CaO-P₂O₅-Na₂O System: A Histomorphometric Study in Rats

Abstract

The effect of boron-containing bioactive glass (BG) particles in the SiO2-CaO-P2O5-Na2O system on the bone formation was studied by histologic, histomorphometric and microchemical evaluation. Wistar rats were used throughout. Under anesthesia, 45S5 BG particles were placed inside the medullary compartment of the tibia (Control), while in the contralateral tibia (Experimental) 45S5.2B BG particles were implanted. The animals were sacrificed 15 days postimplantation. The tibiae were resected, radiographed, and embedded in methyl methacrylate resin. Sections were stained with toluidine blue and analyzed by light microscopy, backscattered scaning electron microscopy and energy-dispersive X-ray analysis (EDX). Histomorphometric determinations were performed. Light microscopy of the histologic sections showed lamellar bone formation surrounding both biomaterials. The histomorphometric study revealed a statistically significant increase in bone tissue around 45S5.2B BG particles. EDX of newly formed bone tissue showed a rise in the Ca:P ratio when 45S5.2B BG particles were employed. The results described in the present study reveal that this boron-containing bioactive glass may be used as scaffold for bone tissue regeneration.

Development of a novel functionally graded membrane containing boron‐modified bioactive glass nanoparticles for guided bone regeneration

“…ALP activity results suggested that 7B‐BG containing bilayered group could stimulate the early osteogenic differentiation marker of hDPSCs better in comparison with the other groups. It has been reported that ionic dissolution products released from the membranes containing B‐modified BG could mediate the differentiation of hDPSCs (El‐Gendy, Yang, Newby, Boccaccini, & Kirkham, ; Gorustovich, Guglielmotti, Porto López, & Cabrini, ).…”

“…Different ions were incorporated into BG compositions, and their effects have been investigated (Nandi, Mahato, Kundu, & Mukherjee, ). Boron (B)‐incorporated BGs are used in regenerative medicine due to their positive effect on release of growth factors and increased turnover of the extracellular matrix (Gorustovich, Guglielmotti, Porto Lopez, & Cabrini, ). Inclusion of B into BGs enhances their biodegradation and bioactivity properties (Fernandes et al, ; Nandi et al, ; Yang et al, ).…”

A novel methodology for imaging new bone formation around bioceramic bone substitutes

“…Overheating and additional bone damage were prevented by using manual rotating impulsion. 7 Boron-modified melt-derived 45S5 bioactive glass (BG) particles (300-350 mm) 8,9 were placed inside the medullary compartment of the tibiae. The wounds were carefully sutured with an absorbable polyglactin 910 suture (Vicryl, Ethicon).…”