Increased PUFA levels in kidney epithelial cells in the course of diclofenac toxicity

Aslan, Mutay; Konuk, Esma Kirimlioglu; Afşar, Ebru; Çeker, Tuğçe; Yılmaz, Çağatay

doi:10.1016/j.tiv.2020.104836

Cited by 9 publications

(9 citation statements)

References 31 publications

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“…The effect of ketoprofen, was qualitatively similar to the previously reported results with firefly luciferases that showed enantioselective thioesterification of CoA to this compound. [ 52,53 ] In the case of p ‐coumaric acid, although it is not a substrate for the formation of acyl‐adenylate by firefly luciferases, [ 3 ] it was partially recognized as a substrate for the thioesterification reaction with CoA by the Z. morio luciferase‐like enzyme. [ 10 ]…”

Section: Resultsmentioning

confidence: 99%

“…[ 1 ] In beetles, luciferases are bifunctional enzymes that catalyze ATP‐dependent oxidation of benzothiazolic luciferin in two steps: (i) ATP‐dependent adenylation of d ‐luciferin with the production of luciferyl‐adenylate, pyrophosphate, and water; and (ii) oxidation using molecular oxygen of luciferyl‐adenylate to produce AMP, CO 2 , and electronically excited oxyluciferin, which decays to emit light. [ 2‐6 ]…”

Section: Introductionmentioning

confidence: 99%

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Selective inhibition of Zophobas morio (Coleoptera: Tenebrionidae) luciferase‐like enzyme luminescence by diclofenac and potential suitability for light‐off biosensing

et al. 2020

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The accumulation of toxic carboxylic compounds may cause severe effects on the environment and living organisms. A luciferase‐like enzyme, previously cloned from the Malpighian tubules of the non‐luminescent Zophobas morio mealworm, displays thioesterification activity with a wide range of carboxylic substrates, and produces weak red luminescence in the presence of ATP and firefly d‐luciferin, a xenobiotic for this organism. To better investigate the function of this enzyme in carboxylic xenobiotic detoxification, we analyzed the inhibitory effect of different xenobiotic carboxylic acids on the luminescence activity of this enzyme, including environmental pollutants and pharmaceutical compounds. Noteworthy, the anti‐inflammatory drug diclofenac severely inhibited this luciferase‐like enzyme luminescence activity, both in in vitro (IC50 20 μM) and in vivo in bacterial cells assays, when compared with other beetle luciferases. Similar results were obtained with its brighter I327S mutant. Kinetic analysis of diclofenac's effect on luminescence activity indicated mixed‐type inhibition for both ATP and d‐luciferin. Modelling studies showed five potential binding sites for diclofenac, including the coenzyme A binding site, which showed one of the highest binding constant. Taken together, these results raise the possibility of using this luciferase‐like enzyme for the development of novel whole‐cell luminescent biosensors for diclofenac and similar drugs.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Selective inhibition of Zophobas morio (Coleoptera: Tenebrionidae) luciferase‐like enzyme luminescence by diclofenac and potential suitability for light‐off biosensing

et al. 2020

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“…Non-steroidal anti-inflammatory drugs like diclofenac are also used to counteract COVID-19-induced fever and myalgia [38]. Furthermore, COVID-19 and cancer patients are at high-risk of acetaminophen-induced hepatotoxicity as reviewed in [39], diclofenac-induced gastrointestinal side effects as reviewed in [40], along with renal toxicity [41]; luckily, TQ administration protects against acetaminophen-induced hepatotoxicity [42]. In a recent study, TQ protected against diclofenac-induced kidney injury [42,43] and ameliorated GIT toxicity with proton pump inhibition activity [44,45].…”

Section: Benefits Of Thymoquinone As An Adjuvant In Covid-19mentioning

confidence: 99%

“…Furthermore, COVID-19 and cancer patients are at high-risk of acetaminophen-induced hepatotoxicity as reviewed in [39], diclofenac-induced gastrointestinal side effects as reviewed in [40], along with renal toxicity [41]; luckily, TQ administration protects against acetaminophen-induced hepatotoxicity [42]. In a recent study, TQ protected against diclofenac-induced kidney injury [42,43] and ameliorated GIT toxicity with proton pump inhibition activity [44,45]. Moreover, TQ counteracted the toxic effects of other supportive therapies like gentamicin [46,47], chloroquine [48] and vancomycin [25] and showed a synergistic effect when administered with ranitidine [44,45].…”

Section: Benefits Of Thymoquinone As An Adjuvant In Covid-19mentioning

confidence: 99%

Thymoquinone: A Tie-Breaker in SARS-CoV2-Infected Cancer Patients?

Elgohary

Elkhodiry

Amin

et al. 2021

Cells

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Since the beginning of the SARS-CoV-2(severe acute respiratory syndrome-coronavirus-2) pandemic, arace to develop a vaccine has been initiated, considering the massive and rather significant economic and healthcare hits that this virus has caused. The pathophysiology occurring following COVID-19(coronavirus disease-2019) infection has givenhints regarding the supportive and symptomatic treatments to establish for patients, as no specific anti-SARS-CoV-2 is available yet. Patient symptoms vary greatly and range from mild symptoms to severe fatal complications. Supportive treatments include antipyretics, antiviral therapies, different combinations of broad-spectrum antibiotics, hydroxychloroquine and plasma transfusion. Unfortunately, cancer patients are at higher risk of viral infection and more likely to develop serious complications due to their immunocompromised state, the fact that they are already administering multiple medications, as well as combined comorbidity compared to the general population. It may seem impossible to find a drug that possesses both potent antiviral and anticancer effects specifically against COVID-19 infection and its complications and the existing malignancy, respectively. Thymoquinone (TQ) is the most pharmacologically active ingredient in Nigella sativa seeds (black seeds); it is reported to have anticancer, anti-inflammatory and antioxidant effects in various settings. In this review, we will discuss the multiple effects of TQ specifically against COVID-19, its beneficial effects against COVID-19 pathophysiology and multiple-organ complications, its use as an adjuvant for supportive COVID-19 therapy and cancer therapy, and finally, its anticancer effects.

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“…The average removal rate of diclofenac was very low (26%) based the sewage treatment process reported by Lindqvist et al (2005). Some researchers focus on its potential toxicity to the environment (Fent et al 2006;Aslan et al 2020;Duarte et al 2020). Oaks et al (2004) referred to diclofenac residues as the cause of vulture population decline in Pakistan.…”

Section: Graphical Abstract Introductionmentioning

confidence: 99%

Electrochemical degradation of DCF by boron-doped diamond anode: degradation mechanism, pathways and influencing factors

Qiu

Fan

et al. 2021

Water Science and Technology

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Nonsteroidal anti-inflammatory drugs (NAIDS) have been widely detected in wastewater and surface water, which indicates the removal of NAIDS by wastewater treatment plants was not efficiency. Electrochemical advanced oxidation technology is considered to be an effective process. This study presents an investigation of the kinetics, mechanism and influencing factors of Diclofenac (DCF) degradation by an electrochemical process with the boron doped diamond anodes. Relative operating parameters and water quality parameters are examined. It appears that the degradation follows the pseudo-first-order degradation kinetics. DCF degradation was accelerated with the increase of pH from 6 to 10. The degradation was promoted by the addition of electrolyte concentrations and current density. HA and HCO3− significantly inhibited the degradation, whereas Cl− accelerated it. According to the inhibition tests, hydroxyl radicals (•OH) and sulfate radicals (SO4•–) contributed 76.5% and 6.5%, respectively, to the degradation. Sodium sulphate remains a more effective electrolyte, compared to sodium nitrate and sodium phosphate, suggesting the quenching effect of nitrate and phosphate on •OH. Major DCF transformation products were identified. According to the degradation products detected by liquid chromatography-mass spectrometry, hydroxylation and decarboxylation are the main pathways to DCF degradation; meanwhile, dechlorination, chlorination and nitro substitution are also included.

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Increased PUFA levels in kidney epithelial cells in the course of diclofenac toxicity

Cited by 9 publications

References 31 publications

Selective inhibition of Zophobas morio (Coleoptera: Tenebrionidae) luciferase‐like enzyme luminescence by diclofenac and potential suitability for light‐off biosensing

Selective inhibition of Zophobas morio (Coleoptera: Tenebrionidae) luciferase‐like enzyme luminescence by diclofenac and potential suitability for light‐off biosensing

Thymoquinone: A Tie-Breaker in SARS-CoV2-Infected Cancer Patients?

Electrochemical degradation of DCF by boron-doped diamond anode: degradation mechanism, pathways and influencing factors

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