Increased Salivary microRNAs That Regulate DJ-1 Gene Expression as Potential Markers for Parkinson’s Disease

Abstract

Small molecule RNAs (microRNAs) are a kind of endogenous, stable, and noncoding RNA molecule that can regulate the expression of target genes such as DJ-1 at the posttranscriptional level. This study aimed to detect the expression of salivary microRNAs and to discover their value as a salivary potential biomarker for Parkinson's disease (PD). Through a case-control study, RT-qPCR technology was used to detect the expression of miR-874 and miR-145-3p in the saliva of 30 PD patients and 30 healthy volunteers. Then we compared the differences in the expression levels of salivary miR-874 and miR-145-3p between the PD group and the control group and analyzed the correlation between the expression of salivary miR-874 and miR-145-3p in terms of age, gender, disease condition, and disease course. We found that salivary miR-874 and miR-145-3p were both positively expressed in the PD group and control group, and their expression in the PD group was higher than that in the control group. The expression of salivary miRNA-874 and miR-145-3p had no clear correlation to age, gender, total RNA concentrations in saliva, the score of UPDRSII, UPDRSIII, olfactory test scale, MMSE, MoCA, Hohn-Yahr stage and disease course. In conclusion, in the PD group and the control group with positive expression, the expression levels of miR-874 and miR-145-3p in the PD group were higher than those in the control group. The detection of miR-874 and miR-145-3p expression in saliva can be used as an auxiliary biomarker for PD.

A Sex Perspective in Neurodegenerative Diseases: microRNAs as Possible Peripheral Biomarkers

“…In another study on salivary samples, Chen and colleagues [ 92 ] enrolled 30 patients with PD and 30 HCs and analyzed microRNAs that regulate the deglycase DJ-1 gene expression, finding no differences between sexes.…”

MicroRNA Dysregulation in Parkinson’s Disease: A Narrative Review

“…The up-regulation of miR-4639-5p led to the down-regulation of DJ-1 protein level hence resulted in severe oxidative stress and neuronal death in MPP + -and rotenone-treated SH-SY5Y cells (Chen et al, 2017). MiR-145-3p and miR-874 are among other miRNAs that were predicted to regulate DJ-1 expression, and both were shown to be highly expressed in the saliva of PD patients (Chen et al, 2020).…”

MicroRNAs, Parkinson’s Disease, and Diabetes Mellitus

“…By comparing the miRNAs identified in this experiment with those previously reported to be associated with DKD, miR-133b was up-regulated in urinary exosomes in patients with type 2 DKD [ 144 ]. miR-145-3p in the PD group was higher than that in the control group [ 145 ]. In rats with type 1 DM, the therapeutic effects of stroke treatment were compared between bone-marrow stromal cells (BMSCs) derived from type 1 DM rats (DM-BMSCs) and BMSCs derived from normal rats (Nor-BMSCs).…”