Increased sensitivity of human lung adenocarcinoma cells to cisplatin associated with downregulated contactin-1

Zhang, Ruijie; Yao, Weirong; Pei, Qian; Li, Yingjie; Jiang, Chaowen; Ao, Zhi; Qian, Guisheng; Wang, Changzheng; Wu, Guohao; Li, Jin; Ji, Fuyun; Xu, Jun

doi:10.1016/j.biopha.2014.11.004

Cited by 18 publications

(31 citation statements)

References 35 publications

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“…The cisplatin-resistant cell line (A549/DDP) was established and verified as described in our previous studies [36-37]. …”

Section: Methodsmentioning

confidence: 99%

“…The cell metastasis ability in each group was calculated using Image-Pro Plus software (Version 5.1, Media Cybernetics, Inc.). The cell invasion ability was evaluated using modified Boyden chambers with filter inserts (pore size of 8 µm, Corning Inc., Life Science) according to our previous method [36]. Invasive cells were observed under a light microscope (Olympus BX51, Japan), and calculated using Image-Pro Plus software (Version 5.1, Media Cybernetics, Inc.).…”

Section: Methodsmentioning

confidence: 99%

“…After the total protein was extracted from cancer cells using RIPA lysis solution (Beyotime, China), protein expression was analyzed by Western blotting assay as described previously [36]. The primary antibodies and secondary antibodies used in this experiment are listed in Table 2.…”

Section: Methodsmentioning

confidence: 99%

“…Furthermore, the elevated expression of CNTN-1 was demonstrated to facilitate metastasis of MKN45 gastric cancer cells via activation of the EMT process [35]. Additionally, in our previous study, silencing CNTN-1 promoted the sensitivity to chemotherapeutic drugs and attenuated metastasis and the invasion ability of A549/DDP (a MDR cell line of NSCLC) cells [36]. The studies mentioned above suggest that CNTN-1 may contribute to the EMT process and resulting chemoresistance in A549/DDP cells as a mediator.…”

Section: Introductionmentioning

confidence: 99%

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CNTN-1 Enhances Chemoresistance in Human Lung Adenocarcinoma Through Induction of Epithelial-Mesenchymal Transition by Targeting the PI3K/Akt Pathway

Zhang

Sun

et al. 2017

Cell Physiol Biochem

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Background/Aims: Chemoresistance has been a major obstacle to the effective treatment of lung cancer. Previously, we found that contactin-1 (CNTN-1) is related to cisplatin resistance in lung adenocarcinoma. Here, we aimed to investigate the underlying mechanism behind the role of CNTN-1 in cisplatin resistance in lung adenocarcinoma. Methods: EMT-associated phenotypes, including alterations in cellular morphology and marker (E-cadherin, N-cadherin and Vimentin) expression, were compared between A549 cells and A549/DDP cells (a cisplatin-resistant cell line of lung adenocarcinoma with abnormal CNTN-1 expression) by using real-time time PCR and Western blotting. Other methods, including CNTN-1 overexpression in A549 cells and CNTN-1 knockdown in A549/DDP cells, were also used to investigate the role of CNTN-1 in mediating the EMT phenotype and thr resulting cisplatin resistance and malignant progression of cancer cells in vitro and in vivo. Results: A549/DDP cells exhibited an EMT phenotype and aggravated malignant behaviors. CNTN-1 knockdown in A549/DDP cells partly reversed the EMT phenotype, increased drug sensitivity, and attenuated the malignant progression whereas CNTN-1 overexpression in A549 cells resulted in the opposite trend. Furthermore, the PI3K/Akt pathway was involved in the effects of CNTN-1 on EMT progression in A549/DDP cells, verified by the xenograft mouse model. Conclusion: CNTN-1 promotes cisplatin resistance in human cisplatin-resistant lung adenocarcinoma through inducing the EMT process by activating the PI3K/Akt signaling pathway. CNTN-1 may be a potential therapeutic target to reverse chemoresistance in cisplatin-resistant lung adenocarcinoma.

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“…The cisplatin-resistant cell line (A549/DDP) was established and verified as described in our previous studies [36-37]. …”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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CNTN-1 Enhances Chemoresistance in Human Lung Adenocarcinoma Through Induction of Epithelial-Mesenchymal Transition by Targeting the PI3K/Akt Pathway

Zhang

Sun

et al. 2017

Cell Physiol Biochem

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“…Both in vitro and passive‐transfer experiments in animal models have demonstrated anti‐CNTN1 antibodies’ pathogenicity in CIDP 11. CNTN1 has also been postulated to play a pivotal role in the development, progression, and pathogenesis of multiple tumors, being its expression considered as a marker of poor prognosis 12, 13, 14, 15, 16. Two of our CIDP patients harboring antibodies against CNTN1, respectively, developed a lymphoma and a colon adenocarcinoma in addition to their neuropathy, one of them concomitantly, and the other several years later.…”

Section: Introductionmentioning

confidence: 99%

Antibodies against cell adhesion molecules and neural structures in paraneoplastic neuropathies

Siles

Martínez‐Hernández

Araque

et al. 2018

Ann Clin Transl Neurol

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ObjectiveParaneoplastic neurological syndromes (PNS) are rare neurological disorders in which ectopic expression of neural antigens by a tumor results in an autoimmune attack against the nervous system. Onconeural antibodies not only guide PNS diagnosis but may also help detecting underlying malignancies. Our project aims to uncover new potential antibodies in paraneoplastic neuropathies (PN).MethodsThirty‐four patients fulfilling diagnostic criteria of possible (n = 9; 26.5%) and definite (n = 25; 73.5%) PN without onconeural antibodies and 28 healthy controls were included in our study. Sera were tested for known antibodies against neural cell adhesion molecules and screened for novel IgG and IgM reactivities against nerve components: dorsal root ganglia (DRG) neurons, motor neurons, and Schwann cells. Patients showing autoantibodies against any of these cell types were used for immunoprecipitation (IP) studies.ResultsOverall, 9 (26.5%) patients showed significant reactivity against DRG neurons, motor neurons, or Schwann cells, whereas 5 (17.9%) healthy controls only showed moderate reactivity. Compared with control sera, serum samples from patients with paraneoplastic sensory‐motor neuropathies had a higher frequency of IgM antibodies against Schwann cells (0% vs. 40%; P = 0.0028). No novel antigens were identified from our IP experiments. Antibodies against the neural adhesion molecules CNTN1, NF155, NF140, NF186, NCAM1, L1CAM, and the CNTN1/CASPR1 complex were not detected in patients with PN. One (2.9%) patient with CIDP and thymoma had CASPR2 antibodies.InterpretationAlmost 30% of patients with PN harbor antibodies targeting neural structures, suggesting that novel neoplasm‐associated antigens remain to be discovered.

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Prognostic implication and immunological value of contactin 1 expression in pan‐cancer

Zhang

Liu

et al. 2022

Clinical and Translational Dis

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Increased sensitivity of human lung adenocarcinoma cells to cisplatin associated with downregulated contactin-1

Cited by 18 publications

References 35 publications

CNTN-1 Enhances Chemoresistance in Human Lung Adenocarcinoma Through Induction of Epithelial-Mesenchymal Transition by Targeting the PI3K/Akt Pathway

CNTN-1 Enhances Chemoresistance in Human Lung Adenocarcinoma Through Induction of Epithelial-Mesenchymal Transition by Targeting the PI3K/Akt Pathway

Antibodies against cell adhesion molecules and neural structures in paraneoplastic neuropathies

Prognostic implication and immunological value of contactin 1 expression in pan‐cancer

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