“…We aimed at partial denaturation of the albumin with urea. It has been reported that at a concentration of 2.4 M, urea albumin starts to unfold [13], and that it undergoes conformational change at 6 M [14]. According to our experimental protocol, urea that had been dissolved in 0.9% NaCl was added to 50 μl of each plasma sample to reach a final urea concentration of 3 M .…”