Increased serum protein levels by Yuanshi Shengmai Chenggu Tablet in treatment of avascular osteonecrosis of the femoral head

Zeng, Jianchun; Deng, Peng; Li, Jie; Feng, Wenjun; Chen, Jinlun; Zeng, Yirong

doi:10.3892/mmr.2017.8119

Cited by 4 publications

(2 citation statements)

References 25 publications

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“…ONFH is a common orthopedic disease that has high morbidity and is difficult to treat. SINFH is the most common type of nontraumatic ONFH, and its rapid progression seriously affects people's lives [7,20]. Core decompression is the most common treatment for early ONFH.…”

Section: Discussionmentioning

confidence: 99%

A network pharmacology approach and validation experiments to investigate the mechanism of the Chinese medicine Wen-Dan Decoction in the treatment of steroid-induced femoral head necrosis

Li²,

Wu³

et al. 2022

Preprint

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Objective: The present work studied the mechanism of action of Wen-Dan Decoction in treating steroid-induced necrosis of the femoral head (SINFH) via network pharmacology and in vivo experiments.Methods: The microarray dataset GSE123568 (peripheral blood), which includes 30 SINFH patients and 10 non-SINFH samples (after using steroids), is included in the Gene Expression Omnibus (GEO) database. Differential expression analysis and weighted gene coexpression network analysis (WGCNA) were used to identify key SINFH-related genes. To investigate the functions of key SINFH-related genes, Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) enrichment analyses were performed. We constructed a protein–protein interaction (PPI) network based on the STRING database. In addition, we screened active components in Wen-Dan Decoction and their possible role in treating hormonal osteonecrosis using the Traditional Chinese Medicine System Pharmacology (TCMSP) Database. Subsequently, the potential mechanisms of action of Wen-Dan Decoction in the treatment of SINFH obtained via network pharmacology analyses were validated in in vivo experiments.Results: A total of 608 DEGs were obtained (230 upregulated, 378 downregulated). In GO functional enrichment analysis, SINFH-related genes were mainly involved in neutrophil activation and immune response, and KEGG pathway analysis revealed that SINFH genes were mainly associated with cytokine receptor interactions, lipids and atherosclerosis, and tuberculosis. In addition, a total of 147 active ingredients were obtained for Wen-Dan Decoction, of which the core ingredient was quercetin, an active ingredient in licorice. Meanwhile, 277 target genes were identified for the treatment of SINFH with Wen-Dan Decoction, and NCF1, PTGS2, and RUNX2 were selected as core target genes. Furthermore, the results of quantitative real-time polymerase chain reaction (qRT–PCR) on peripheral blood and bone tissue samples from SINFH patients and non-SINFH samples showed that PGTS2 and NCF1 were upregulated in patient blood and necrotic bone tissues. In quercetin-treated mice, the appearance of femoral head necrosis was significantly improved, and micro-CT suggested that the bone density was significantly better. Finally, the results of qRT–PCR analysis of peripheral blood and femoral bone tissue from a mouse model of SINFH treated with quercetin showed that PGTS2 and NCF1 levels were downregulated compared with those of controls, which was consistent with the results of bioinformatics analysis.Conclusion: This study provides new insights into the genes and related pathways involved in SINFH and shows that PTGS2 and NCF1 could be used as potential drug targets in SINFH.

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Section: Discussionmentioning

confidence: 99%

A network pharmacology approach and validation experiments to investigate the mechanism of the Chinese medicine Wen-Dan Decoction in the treatment of steroid-induced femoral head necrosis

Li²,

Wu³

et al. 2022

Preprint

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“…Recent reports have shown that the occurrence of ANFN could be greatly decreased by an early intervention on high-risk crowds of ANFN who use hormones such as steroid and alcohol [ 16 ]. However, the composition of the serum is very complex [ 17 ]. It contains high-abundance proteins like albumin and immunoglobulins (mainly IgG), as well as low abundance proteins that are secreted by tissue or cells [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Screening of Serum Protein Markers for Avascular Osteonecrosis of Femoral Head Differentially Expressed after Treatment with Yuanshi Shengmai Chenggu Tablets

Deng

Zeng

et al. 2018

BioMed Research International

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Avascular necrosis of the femoral head (ANFH) is an a frequently occurring orthopaedic disease with high morbidity. Traditional Chinese Medicine (TCM) Yuanshi Shengmai Chenggu Tablet is a valid prescription for treating steroid-induced femoral head necrosis. However, there are rare investigations about the serum protein marker expression after the acting of drugs on hormone and TCM. In the present study, we aimed to systematically discover and validate the serum biomarkers expression difference in patients with steroid-induced avascular necrosis of femoral head (SANFH) after taking Yuanshi Shengmai Chenggu Tablets (SANFH-TCM), so as to reveal the action mechanism of TCM from the molecular level by using isobaric tags for relative and absolute quantification (iTRAQ) with multiple reaction monitoring quantification. Significant differences in fibrinogen alpha, fibrinogen beta, fibrinogen gamma, fibronectin, C-reactive protein, apolipoprotein A, apolipoprotein D, and apolipoprotein E were found among SANFH, SANFH-TCM, and healthy controls. Therefore, our study proposes potential biomarkers for SANFH diagnosis and for the prognosis of femoral head necrosis after Traditional Chinese Medicine treatment.

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Taohong Siwu decoction for femoral head necrosis

et al. 2020

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Backgrounds: Femoral head necrosis is one of the most common orthopedic diseases which can be diagnosed in all ages with different reasons. Taohong Siwu decoction (TSD) has been widely used in the treatment of femoral head necrosis. However, as far as we know, there is still a lack of supporting evidence regarding the efficacy of TSD for femoral head necrosis. Therefore, this protocol aims to evaluate the effectiveness and safety of TSD for femoral head necrosis. Methods: Eight electronic databases, including PubMed, the Cochrane Central Register of Controlled Trials, EMBASE, Web of Science, Chinese Biomedical Literature Database, China National Knowledge Infrastructure, Technology Periodical database, (Chinese Scientific Journal Database) and Wanfang Database will be searched from the time when the respective databases were established to January 2020. Randomized controlled trials of TSD in the treatment of femoral head necrosis will be collected. After evaluating the quality of methodology and extracting valid data, the final meta-analysis will be carried out with software Revman 5.3. Ethics and dissemination: The results of this systematic review will offer implications of the use of TSD treatment for Femoral Head Necrosis. It uses aggregated published data instead of individual patient data and does not require an ethical board review and approval. The findings will be published in a peer-reviewed journal and disseminated in conference presentations. Results: The results of this study will offer implications of the use of TSD treatment for FHN with this meta-analysis. Conclusion: The conclusion of this study will provide recent evidence to assess whether TSD is effective and safe in the treatment of FHN.

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Increased serum protein levels by Yuanshi Shengmai Chenggu Tablet in treatment of avascular osteonecrosis of the femoral head

Cited by 4 publications

References 25 publications

A network pharmacology approach and validation experiments to investigate the mechanism of the Chinese medicine Wen-Dan Decoction in the treatment of steroid-induced femoral head necrosis

A network pharmacology approach and validation experiments to investigate the mechanism of the Chinese medicine Wen-Dan Decoction in the treatment of steroid-induced femoral head necrosis

Screening of Serum Protein Markers for Avascular Osteonecrosis of Femoral Head Differentially Expressed after Treatment with Yuanshi Shengmai Chenggu Tablets

Taohong Siwu decoction for femoral head necrosis

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