Increased survival time in mice vaccinated with a branched lysine multiple antigenic peptide containing B- and T-cell epitopes from T. gondii antigens

Wang, Yanhua; Men, Wang; Wang, Guangxiang; An-na, Pang; Bao-quan, FU; Yin, Hong; Zhang, Delin

doi:10.1016/j.vaccine.2011.09.016

Cited by 42 publications

(31 citation statements)

References 52 publications

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“…The first immunization primes the immune response and subsequent immunizations trigger the further expansion of antigen specific cells and selection of cells with high antigen avidity [40]. Synthetic MAP contains a high concentration of the relevant antigen to induce immune responses to predefined epitopes and leads to protective immunity [25, 41]. Meng et al [5] used the DNA prime-peptide boost vaccination regimen to enhance the effectiveness of T. gondii DNA vaccines, which produced a stronger immune response and better protection compared to priming with polypeptide and boosting with DNA.…”

Section: Discussionmentioning

confidence: 99%

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Protection via a ROM4 DNA vaccine and peptide against Toxoplasma gondii in BALB/c mice

et al. 2017

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Background Toxoplasma gondii (T. gondii) is an obligate intracellular protozoan parasite with a broad host range including most warm-blooded animals, including humans. T. gondii surface antigen 1 (SAG1) is a well-characterized T. gondii antigen. T. gondii expresses five nonmitochondrial rhomboid intramembrane proteases, TgROM1-5. TgROM4 is uniformly distributed on the surface of T. gondii and involved in regulating MIC2, MIC3, MIC6, and AMA1 during T. gondii invasion of host cells. Bioinformatics have predicted ROM4 B-cell and T-cell epitopes. Immunization strategy is also a key factor in determining the effectiveness of the immune response and has gained increasing attention in T. gondii vaccine research. In this study, we used a DNA prime-peptide boost vaccination regimen to assess the protective efficacy of various vaccination strategies using TgROM4.MethodsWe identified a polypeptide (YALLGALIPYCVEYWKSIPR) using a bioinformatics approach, and immunized mice using a DNA-prime and polypeptide-boost regimen. BALB/c mice were randomly divided into six groups, including three experimental groups (peptide, pROM4 and pROM4/peptide) and three control groups (PBS, pEGFP-C1 and pSAG1). Mice were then immunized intramuscularly four times. After immunization, IgG and cytokine productions were determined using enzyme-linked immunosorbent assays. The survival time of mice was evaluated after challenge with tachyzoites of T. gondii RH strain. Additionally, the number of cysts in the brain was determined after intragastric challenge with cysts of T. gondii PRU strain.ResultsMice vaccinated with different immunization regimens (peptide, pROM4 and pROM4/peptide) elicited specific humoral and cellular responses, with high levels of IgG, IgG2a, and interferon (IFN)-γ. Moreover, IgG, IgG2a and IFN-γ levels were highest in the pROM4/peptide group. Immunized mice, especially those in the pROM4/peptide group, had prolonged survival times after challenge with tachyzoites and reduced numbers of brain cysts after infection compared with negative controls.ConclusionA DNA prime-peptide boost regimen based on ROM4 elicited the highest level of humoral and cellular immune responses among immunization regimens, and may be a promising approach to increase the efficacy of DNA immunization.

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Section: Discussionmentioning

confidence: 99%

“…In particular, a prime-boost vaccination strategy induced a more efficient humoral and cellular immune response [22–24]. Studies have shown that a synthetic multiple antigenic peptides (MAP) vaccine induces protective immunity against intracellular parasites, including T. gondii [25, 26]. …”

Section: Introductionmentioning

confidence: 99%

Protection via a ROM4 DNA vaccine and peptide against Toxoplasma gondii in BALB/c mice

et al. 2017

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“…In contrast to DNA vaccines, synthetic multiple antigen peptide (MAP) vaccines are an effective and new approach to deliver multiple T-cell and B-cell epitopes as the constituents of a single immunogen, which contains a high concentration of the relevant antigen for inducing immune responses to predefined epitopes [15]. Synthetic MAP vaccines have been shown to elicit better cell-mediated immunity by focusing the host immune response on epitopes known to play a role in protective immunity [16,17]. Recently, several studies have demonstrated the power of synthetic polypeptides vaccines in eliciting protective immunity to intracellular parasites such as Plasmodium falciparum [18], Schistosoma mansoni [19], T. gondii [17], and Paracoccidioides brasiliensis [20].…”

Section: Introductionmentioning

confidence: 99%

“…Synthetic MAP vaccines have been shown to elicit better cell-mediated immunity by focusing the host immune response on epitopes known to play a role in protective immunity [16,17]. Recently, several studies have demonstrated the power of synthetic polypeptides vaccines in eliciting protective immunity to intracellular parasites such as Plasmodium falciparum [18], Schistosoma mansoni [19], T. gondii [17], and Paracoccidioides brasiliensis [20]. …”

Section: Introductionmentioning

confidence: 99%

DNA prime and peptide boost immunization protocol encoding the Toxoplasma gondii GRA4 induces strong protective immunity in BALB/c mice

et al. 2013

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BackgroundToxoplasma gondii is a widespread intracellular parasite, which infects most vertebrate animal hosts and causes zoonotic infection in humans. Vaccine strategy remains a promising method for the prevention and control of toxoplasmosis. T. gondii GRA4 protein has been identified as a potential candidate for vaccine development. In our study, we evaluated the immune response induced by four different immunization vaccination strategies encoding TgGRA4.MethodsBALB/c mice were intramuscularly (i.m.) immunized four times according to specific immunization schedules. Generally, mice in experimental groups were immunized with polypeptide, pGRA4, peptide/DNA, or DNA/peptide, and mice in the control groups were injected with PBS or pEGFP. After immunization, the levels of IgG antibodies and cytokine productions were determined by enzyme-linked immunosorbent assays (ELISA). The survival time of mice was also evaluated after challenge infection with the highly virulent T. gondii RH strain.ResultsThe results showed that mice vaccinated with different immunization regimens (polypeptide, pGRA4, peptide/DNA, or DNA/peptide) elicited specific humoral and cellular responses, with high levels of total IgG, IgG2a isotype and gamma interferon (IFN-γ), which suggested a specific Th1 immunity was activated. After lethal challenge, an increased survival time was observed in immunized mice (11.8 ± 4.8 days) compared to the control groups injected with PBS or pEGFP (P < 0.05). Mice injected with PBS or pEGFP died within 8 days, and there was no significant difference in the protection level in two groups (P > 0.05).ConclusionsThese results demonstrated that this DNA prime and peptide boost immunization protocol encoding the TgGRA4 can elicit the highest level of humoral and cellular immune responses compared to other immunized groups, which is a promising approach to increase the efficacy of DNA immunization.

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“…Furthermore, significant differences were observed at 2, 3, and the 166 last dpi (P < 0.05). Accordingly, compared to the control group, infected mice that received the and produced a highly effective and significant protection against T. gondii infection (37,38). 213 For the challenge experiments, we injected the mice with 1 × 10 4 live tachyzoites.…”

mentioning

confidence: 99%

Excretory–secretory antigens: A suitable candidate for immunization against ocular toxoplasmosis in a murine model

Deilami

Daryani

Ahmadpour

et al. 2014

Comparative Immunology, Microbiology and Infectious Diseases

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Increased survival time in mice vaccinated with a branched lysine multiple antigenic peptide containing B- and T-cell epitopes from T. gondii antigens

Cited by 42 publications

References 52 publications

Protection via a ROM4 DNA vaccine and peptide against Toxoplasma gondii in BALB/c mice

Protection via a ROM4 DNA vaccine and peptide against Toxoplasma gondii in BALB/c mice

DNA prime and peptide boost immunization protocol encoding the Toxoplasma gondii GRA4 induces strong protective immunity in BALB/c mice

Excretory–secretory antigens: A suitable candidate for immunization against ocular toxoplasmosis in a murine model

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