Increased TFF1 trefoil protein expression in <i>Opisthorchis viverrini</i>‐associated cholangiocarcinoma is important for invasive promotion

Thuwajit, Peti; Chawengrattanachot, Watinee; Thuwajit, Chanitra; Sripa, Banchob; May, Felicity E. B.; Westley, Bruce R.; Tepsiri, Nisana; Paupairoj, Anucha; Chau-in, Siri

doi:10.1111/j.1872-034x.2007.00045.x

Cited by 18 publications

(17 citation statements)

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“…Tissue specimens were kept as paraffin-embedded formaldehyde fixed. The CCA cell lines, KKU-100 and KKU-M213, were established in the Department of Pathology, Faculty of Medicine, Khon Kaen University, Thailand (Sripa et al 2005;Thuwajit et al 2007). KKU-100 and KKU-M213 cells were developed from poorly and well-differentiated CCA tissues, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…A previous study showed that TFF1 is not expressed in hepatocytes and minimally expressed in normal cholangiocytes (Sasaki et al 2003). However, a high expression of TFF1 has been reported in CCA cells (Thuwajit et al 2007). The in vitro studies have shown the stimulation of TFF1 on cell invasion in CCA (Thuwajit et al 2007) and breast cancer (Prest et al 2002).…”

Section: Introductionmentioning

confidence: 95%

“…The immunostaining method has been previously described (Thuwajit et al 2007). The intensity of the expression was classified semi-quantitatively into four groups, on the basis of the percentage of positively stained cells: 0, negative; ?1, 1-25 %; ?2, 26-50 %; and ?3 [ 50 %.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…However, a high expression of TFF1 has been reported in CCA cells (Thuwajit et al 2007). The in vitro studies have shown the stimulation of TFF1 on cell invasion in CCA (Thuwajit et al 2007) and breast cancer (Prest et al 2002). TFF1 gene contains an estrogen-responsive element, which could promote transcription by estrogen (Rio and Chambon 1990).…”

Section: Introductionmentioning

confidence: 97%

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Estrogen is increased in male cholangiocarcinoma patients’ serum and stimulates invasion in cholangiocarcinoma cell lines in vitro

Hunsawong

Singsuksawat

In-chon

et al. 2012

J Cancer Res Clin Oncol

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Section: Immunohistochemistrymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

See 2 more Smart Citations

Estrogen is increased in male cholangiocarcinoma patients’ serum and stimulates invasion in cholangiocarcinoma cell lines in vitro

Hunsawong

Singsuksawat

In-chon

et al. 2012

J Cancer Res Clin Oncol

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“…To evaluate the possible biological functions correlated to the metal binding ability of TFF1, we studied the effects of copper binding on the well-described motogenic activity of TFF1 [26,27]. To this aim, we used an inducible hyperexpressing clone of the gastric carcinoma cell line AGS (AGS-AC1) (Fig.…”

Section: Copper (Ii) Binding Affinitymentioning

confidence: 99%

Copper binds the carboxy-terminus of trefoil protein 1 (TFF1), favoring its homodimerization and motogenic activity

Tosco

Monti

Fontanella

et al. 2010

Cell. Mol. Life Sci.

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Trefoil protein 1 (TFF1) is a small secreted protein belonging to the trefoil factor family of proteins, that are present mainly in the gastrointestinal (GI) tract and play pivotal roles as motogenic factors in epithelial restitution, cell motility, and other incompletely characterized biological processes. We previously reported the up-regulation of TFF1 gene in copper deficient rats and the unexpected property of the peptide to selectively bind copper. Following the previous evidence, here we report the characterization of the copper binding site by fluorescence quenching spectroscopy and mass spectrometric analyses. We demonstrate that Cys58 and at least three Glu surrounding residues surrounding it, are essential to efficiently bind copper. Moreover, copper binding promotes the TFF1 homodimerization, thus increasing its motogenic activity in in vitro wound healing assays. Copper levels could then modulate the TFF1 functions in the GI tract, as well as its postulated role in cancer progression and invasion.

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Organoid‐Based Human Stomach Micro‐Physiological System to Recapitulate the Dynamic Mucosal Defense Mechanism

et al. 2023

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Several stomach diseases are attributed to the dysregulation of physiological function of gastric mucosal barrier by pathogens. Gastric organoids are a promising tool to develop treatment strategies for gastric infections. However, their functional features of in vivo gastric mucosal barrier and host–microbe interactions are limited due to the lack of physiological stimuli. Herein, a human stomach micro‐physiological system (hsMPS) with physiologically relevant gastric mucosal defense system is described based on the combination of organoid and MPS technology. A fluid flow enhanced epithelial‐mesenchymal interaction in the hsMPS enables functional maturation of gastric epithelial cells, which allows for the recreation of mesh‐like mucus layer containing high level of mucus protective peptides and well‐developed epithelial junctional complexes. Furthermore, gastroprotection mechanisms against Helicobacter pylori (H. pylori) are successfully demonstrated in this system. Therefore, hsMPS represents a new in vitro tool for research where gastric mucosal defense mechanism is pivotal for developing therapeutic strategies.

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Increased TFF1 trefoil protein expression in Opisthorchis viverrini‐associated cholangiocarcinoma is important for invasive promotion

Abstract: TFF1 showed co-expression with MUC5AC in CCA tissues and invasive stimulating activity in vitro. These results may indicate a role for TFF1 in promoting tumor invasion in CCA.

Cited by 18 publications

References 50 publications

Estrogen is increased in male cholangiocarcinoma patients’ serum and stimulates invasion in cholangiocarcinoma cell lines in vitro

Estrogen is increased in male cholangiocarcinoma patients’ serum and stimulates invasion in cholangiocarcinoma cell lines in vitro

Copper binds the carboxy-terminus of trefoil protein 1 (TFF1), favoring its homodimerization and motogenic activity

Organoid‐Based Human Stomach Micro‐Physiological System to Recapitulate the Dynamic Mucosal Defense Mechanism

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