Increased Th17 cells in flow cytometer-sorted CD45RO-positive memory CD4 T cells from patients with systemic lupus erythematosus

Abstract

ObjectivesTh17/IL-17 dysregulation is involved in human autoimmunity, and recent evidence suggests the character of long-lived differentiated memory cells in Th17. By directly measuring the peripheral blood mononuclear cells (PBMC), elevated circulating frequencies of Th17 cells have been reported in systemic lupus erythematosus (SLE) with inconsistent results regarding the correlation with disease activities. In this study, the association between circulating Th17 frequencies and disease activities or laboratory parameters was examined in flow cytometer-sorted CD45RO-positive memory CD4 T cells from SLE.MethodsPBMC samples were obtained from 48 female lupus patients and another 48 age- and sex-matched healthy individuals. We examined frequencies of Th17 cells by sorting the purified CD4 T cells bearing the CD45RO marker, followed by intracellular IL-17A staining after in vitro activation. Frequencies of Th1 and TFoxp3 cells were also measured by intracellular IFN-γ and Foxp3 staining, respectively. The SLE disease activity index (SLEDAI) and other laboratory parameters were further correlated with frequencies of different T cell subsets.ResultsIn SLE, increased frequencies of Th17 cells were found with a positive correlation in SLEDAI. Higher frequencies of Th17 cells were found in lupus nephritis. There was a positive correlation between frequencies of Th17 cells and daily proteinuria amount.ConclusionsBy examining the sorted CD45RO-positive memory CD4 T cells, we confirm the dysregulation of Th17/IL-17 in SLE, implicating the potential to treat lupus patients with selective IL-17/IL-17R blockades.

The T helper type 17/regulatory T cell paradigm in pregnancy

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Panax notoginseng saponins reverse P-gp-mediated steroid resistance in lupus: involvement in the suppression of the SIRT1/FoxO1/MDR1 signalling pathway in lymphocytes

“…Thus, PNS reversed the P-gp-mediated SR by suppressing the SIRT1/ FoxO1/MDR1 signalling cascade. An increased frequency of Th17 cells was found in lupus, together with a positive correlation to the SLEDAI [55]. Th17 cells are related to SR.…”

Th17 Cell Differentiation Induced by Cytopathogenic Biotype BVDV-2 in Bovine PBLCs

“…Furthermore, we also found that IL22, which together with IL17A and IL17F belongs to the Th17 effector molecules, was the most obviously differentially expressed gene, at 521 folds higher than the NC group. Generally, the production of IL17A has been recognized as the marker of Th17 cell differentiation [44][45][46][47]. In order to determine whether the high levels of pro-inflammatory cytokines were caused by Th17 cell differentiation after BVDV infection, we measured the Th17 cell differentiation by flow cytometry and the production of IL17A by qRT-PCR and western blotting.…”