Increased thymidine uptake by methylcholanthrene‐treated C3H/10T1/2 cells

Abstract

The uptake of 3H-thymidine in post-confluent cultures of methylcholanthrene-transformed C3H/10T1/I mouse embryo cells was markedly higher than in their non-transformed counterparts. In a reconstruction experiment as few as 2% transformed cells could be detected by increased thymidine uptake. Measurements made at various times up to 110 days in multi-chambered plates revealed that after 25 days methylcholanthrene-treated cultures incorporated significantly more thymidine than the acetone-treated controls. This increased uptake correlated with the appearance of Type III transformed foci.

True-to-scale DNA-density maps correlate with major accessibility differences between active and inactive chromatin

“…Cell cultures, fixation, and staining protocols h-TERT BJ1 cells, an immortalized diploid human cell line from human foreskin fibroblasts (ATCC #CRL-2522); HeLa cells, a human cervix carcinoma derived cell line with a near-triploid karyotype (Landry et al, 2013), and C3H 10T1/2 cells, derived from a murine embryo fibroblast cell line (ATCC #CCL-226) (Bairstow and Heidelberger, 1975) were cultured in DMEM with 5% CO2 at 37 °C. Undifferentiated female mouse embryonic stem cells (ESC line 16.7, 42,XX; +6;+8, kindly provided by J. Lee, Harvard Medical School) were cultivated under feeder-free conditions on gelatinized cover slips in KO-DMEM (Invitrogen) as described in details in (Smeets et al, 2014).…”