Increased Tissue Thromboplastin Activity in Monocytes of Patients with Meningococcal Infection: Related to an Unfavourable Prognosis

Østerud, Bjarne; Flægstad, Trond

doi:10.1055/s-0038-1657303

Cited by 309 publications

(177 citation statements)

References 1 publication

Supporting

Mentioning

170

Contrasting

Unclassified

Order By: Relevance

“…13 TF is implicated in numerous pathologic conditions including atherosclerosis, 14 postoperative thrombosis, 15 and sepsis-linked disseminated intravascular coagulation. 16 TF is constitutively expressed on the surface of several extravascular cell types, 17 and it is transiently expressed on the plasmalemma of macrophages, endothelial cells, and monocytes in response to a number of inflammatory agents, including bacterial lipopolysaccharide (LPS). 18 We demonstrate here that Grp78 is present on endothelium overlying the atherosclerotic plaque and on foam cells within the plaque.…”

Section: See Page 1529mentioning

confidence: 99%

Regulation of Tissue Factor–Mediated Initiation of the Coagulation Cascade by Cell Surface Grp78

Bhattacharjee

Ahamed

Pedersen

et al. 2005

ATVB

View full text Add to dashboard Cite

Objective-To test the hypothesis that Grp78 negatively regulates cell surface tissue factor (TF) procoagulant activity and whether this is mediated by physical interaction. Methods and Results-Biopanning with phage-displayed peptidyl libraries has identified peptide probes that bind selectively in vivo to the surface of atherosclerotic plaque endothelium. The highest affinity peptide, EKO130, binds 78-kDa glucose regulated protein (Grp78). Grp78 participates in numerous pathological processes, including the regulation of the coagulation cascade, but the mechanism of Grp78 regulation of coagulation is unknown. To characterize this function, we analyzed the effect of Grp78 on TF-mediated procoagulant activity on murine brain endothelial cells (bEND.3) and macrophage-like (RAW) cells, which are relevant in mediation of atherothrombosis. We show that Grp78 is present on the surface of endothelium and monocyte/macrophage-like cells in atherosclerotic lesions. Inhibition of Grp78 resulted in increased procoagulant activity. We demonstrate that Grp78 negatively regulates procoagulant activity by interacting physically with the TF extracellular domain on the cell surface. Conclusions-The

show abstract

Section: See Page 1529mentioning

confidence: 99%

Regulation of Tissue Factor–Mediated Initiation of the Coagulation Cascade by Cell Surface Grp78

Bhattacharjee

Ahamed

Pedersen

et al. 2005

ATVB

View full text Add to dashboard Cite

show abstract

“…Mononuclear cells from patients with meningococcemia also show TF activity, that correlates with the prognosis of the disease [2]. Since anti-TF antibodies prevent or attenuate endotoxin-and TFinduced disseminated intravascular coagulation (DIC) in animal experimental models [3,4], the role of TF in triggering DIC in Gram-negative septic shock appears clear.…”

Section: Introductionmentioning

confidence: 99%

Interleukin‐10 inhibits endotoxin‐induced tissue factor mRNA production by human monocytes

et al. 1993

View full text Add to dashboard Cite

In Gram-negative septic shock, human monocytes synthesize and express on their cytoplasmic membrane tissue factor (TF), a potent activator of the coagulation cascades. The role of TF in triggering disseminated intravascular coagulation (DIC) in these patients appears to be clear. We report the suppressive effect of interleukin-10 (IL-10) on endotoxin-induced TF activity and antigen levels, and on the expression of TF mRNA levels in human monocytes. These results emphasize the potential therapeutic value of this cytokine in septic shock, a condition still associated with a high mortality rate.

show abstract

“…While it is known that colorectal cancer expresses elevated levels of cellular TF (Szczepanski, 1988) and that higher levels of free TF were observed for colorectal tissues compared to breast tissues (El-Baruni, 1990), there is no obvious reason to explain this phenomenon. Inflammation is also known to significantly enhance mTF production and colorectal cancer is often associated with ulceration and bacterial infection which is known to stimulate mTF expression (Østerud and Flaegstad, 1983). Another important factor which may have contributed to this finding is that most colon cancer patients present at a later stage of disease progression and the tumours are physically larger, particularly in relation to breast cancer which is subject to screening.…”

Section: Discussionmentioning

confidence: 99%

The significance of measuring monocyte tissue factor activity in patients with breast and colorectal cancer

1999

View full text Add to dashboard Cite

Summary Monocytes express tissue factor (mTF) in several conditions including cancer where levels may be valuable in assessing tumour presence and progression. Using a two-stage kinetic chromogenic assay (KCA), mTF levels were measured in controls [normal subjects (n = 60) and patients undergoing hernia repair or cholecystectomy (n = 60)], in patients with benign and malignant disease of the breast (n = 83) and of the large bowel (n = 62). This was performed under fresh (resting) conditions and after incubation for 6 h without (unstimulated) and with (stimulated) Escherichia coli endotoxin. The malignant groups showed higher mTF levels than each of the three controls for resting (P < 0.05 breast, P < 0.05 colorectal) unstimulated (P < 0.05 breast, P < 0.05 colorectal) and stimulated cells (P < 0.001 breast, P < 0.01 colorectal). Similarly, the benign inflammatory groups had higher mTF levels than controls for resting (P < 0.05 colorectal), unstimulated (P < 0.05 colorectal) and stimulated cells (P < 0.01 breast, P < 0.01 colorectal). There was no significant difference between malignant and benign inflammatory groups in each organ. mTF levels showed an increase corresponding to that of histological tumour progression and were higher in non-surviving patients. In conclusion, mTF levels are raised in malignant and inflammatory disease compared to controls and patients with non-inflammatory conditions. Stimulated cells give better discrimination between the groups and may be of value in identifying high risk individuals. mTF levels showed an association with tumour grade or stage and the patients' survival time.

show abstract

Increased Tissue Thromboplastin Activity in Monocytes of Patients with Meningococcal Infection: Related to an Unfavourable Prognosis

Cited by 309 publications

References 1 publication

Regulation of Tissue Factor–Mediated Initiation of the Coagulation Cascade by Cell Surface Grp78

Regulation of Tissue Factor–Mediated Initiation of the Coagulation Cascade by Cell Surface Grp78

Interleukin‐10 inhibits endotoxin‐induced tissue factor mRNA production by human monocytes

The significance of measuring monocyte tissue factor activity in patients with breast and colorectal cancer

Contact Info

Product

Resources

About