Increased urinary exosomal microRNAs in children with idiopathic nephrotic syndrome

Chen, Tingting; Wang, Cheng; Yu, Han; Ding, Meng; Zhang, Cuiping; Lu, Xiaolan; Zhang, Chenyu

doi:10.1016/j.ebiom.2018.11.018

Cited by 56 publications

(43 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Evidence shows that the level of miR-30 family is altered with NS progression and therapy. 22,23 In serum and urine samples, 23 and urinary exosomes 24 of children with active NS, increased concentration of miR-30a-5p was observed, while its level tended to decrease with the remission of patients. In contrast to these results, downregulated levels of miR-30 family were reported in podocytes of FSGS cases 17 and kidney tissue of children with FSGS but not MGN 21 in comparison to normal controls.…”

Section: Discussionmentioning

confidence: 98%

<p>Expression Levels of miR-30c and miR-186 in Adult Patients with Membranous Glomerulonephritis and Focal Segmental Glomerulosclerosis</p>

Hejazian¹,

Ardalan²,

Shoja

et al. 2020

IJNRD

View full text Add to dashboard Cite

Background: Nephrotic syndrome is a common renal problem with different histopathogenesis. MicroRNAs are reported to be involved in the pathophysiology of the syndrome. The aim of this study was to study the levels of miR-30c and miR-186 in NS patients. Methods: Sixty patients with primary NS (membranous glomerulonephritis (MGN, N=30) and focal segmental glomerulosclerosis (FSGS, N=30)) and 24 healthy volunteers were included. Expression levels of the miR-30c and miR-186 were evaluated in plasma and peripheral blood mononuclear cell (PBMC) samples of adult patients with NS using real-time PCR. Moreover, an in-silico analysis was performed to understand the signaling pathways and biological procedures that may be regulated by these miRNAs. Results: In the MGN group, significantly elevated levels of miR-30c and miR-186 were observed in PBMC (P= 0.037) and plasma (P= 0.035) samples, respectively. Moreover, there was a significant increase in miR-30c levels in PBMC samples of the FSGS group when compared to healthy controls (P= 0.004). In ROC curve analysis, combined levels of the studied miRNAs could discriminate cases from controls in plasma and blood cells (AUC≥0.72, P<0.05). Conclusion: A panel of miRNAs may be potential biomarkers in plasma and PBMCs samples of NS patients with different subclasses. More investigations are needed with a large sample size to validate the diagnostic values of the reported miRNAs.

show abstract

Section: Discussionmentioning

confidence: 98%

<p>Expression Levels of miR-30c and miR-186 in Adult Patients with Membranous Glomerulonephritis and Focal Segmental Glomerulosclerosis</p>

Hejazian¹,

Ardalan²,

Shoja

et al. 2020

IJNRD

View full text Add to dashboard Cite

show abstract

“…LncRNA growth arrest-specific 5 (GAS5) mediated by exosomes was also identified as a promising serum-based biomarker for early non-small-cell lung cancer (NSCLC) diagnosis (77). It is widely proven that some exosomal DNAs, RNAs, proteins, and other bioactive molecules are emerging as critical and ideal noninvasive biomarkers for various benign and malignant diseases (78)(79)(80)(81)(82).…”

Section: Exosome Uptake and Functionmentioning

confidence: 99%

Exosome-Derived LncRNAs in Lung Cancer

Fan

Sun

2020

Front. Oncol.

View full text Add to dashboard Cite

As extracellular vesicles, exosomes are released from most cells to perform cell-cell communication. Recent studies have shown that exosomes could be released into tumor microenvironment and blood to promote tumor progression through packaging and transmitting various bioactive molecules, such as cholesterol, proteins, lipids, miRNAs, mRNAs, and long non-coding RNAs (lncRNAs) to distant cells. LncRNAs have emerged as a major class of non-coding transcripts. A lot of LncRNAs have been discovered during the past few years of research on genomics. They have been proven to participate in various biological functions and disease processes through multiple mechanisms. In this review, we analyzed the role of exosome-derived lncRNAs in lung carcinogenesis and metastasis. We also highlight opportunities for the clinical potential of exosomes with specific lncRNAs as biomarkers and therapeutic intervention in lung cancer.

show abstract

“…In the pediatric population, a urinary exosomal miRNA profile of upregulated miR-194-5p, miR-146b-5p, miR-378a-3p, miR-23b-3p, and miR-30a-5p has been identified in various histological patterns of injury in idiopathic nephrotic syndrome [ 66 ]. At the same time, miR-193a levels may be useful in distinguishing between pediatric primary FGSG and MCD [ 67 ].…”

Section: Resultsmentioning

confidence: 99%

Exosomes in Nephropathies: A Rich Source of Novel Biomarkers

et al. 2020

View full text Add to dashboard Cite

The biomarkers commonly utilized in diagnostic evaluations of kidney disease suffer from low sensitivity, especially in the early stages of renal damage. On the other hand, obtaining a renal biopsy to augment clinical decision making can lead to potentially serious complications. In order to overcome the shortcomings of currently available diagnostic tools, recent studies suggest that exosomes, cell-secreted extracellular vesicles containing a large array of active molecules to facilitate cell-to-cell communication, may represent a rich source of novel disease biomarkers. Because of their endocytic origin, exosomes carry markers typical for their parent cells, which could permit the localization of biochemical cellular alterations in specific kidney compartments. Different types of exosomes can be isolated from noninvasively obtained biofluids; however, in the context of kidney disease, evidence has emerged on the role of urinary exosomes in the diagnostic and predictive modeling of renal pathology. The current review summarizes the potential application of exosomes in the detection of acute and chronic inflammatory, metabolic, degenerative, and genetic renal diseases.

show abstract

Increased urinary exosomal microRNAs in children with idiopathic nephrotic syndrome

Cited by 56 publications

References 41 publications

<p>Expression Levels of miR-30c and miR-186 in Adult Patients with Membranous Glomerulonephritis and Focal Segmental Glomerulosclerosis</p>

<p>Expression Levels of miR-30c and miR-186 in Adult Patients with Membranous Glomerulonephritis and Focal Segmental Glomerulosclerosis</p>

Exosome-Derived LncRNAs in Lung Cancer

Exosomes in Nephropathies: A Rich Source of Novel Biomarkers

Contact Info

Product

Resources

About