Increasing antioxidant levels in tomatoes through modification of the flavonoid biosynthetic pathway

Verhoeyen, Martine; Bovy, Arnaud; Collins, Geoff J.; Muir, Shelagh R.; Robinson, Susan; Vos, C. H. Ric De; Colliver, S. P.

doi:10.1093/jxb/erf044

Cited by 215 publications

(156 citation statements)

References 23 publications

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“…Epidemiological studies have consistently shown an inverse association between consumption of some nutritional flavonoids and the risk of human cancers at many sites [6][7][8][9]. NAR has been widely studied and has been reported to be an antioxidant [10,11] …”

Section: Introductionmentioning

confidence: 99%

Formulation and Evaluation of Polymeric Nanosuspension of Naringenin

Sumathi¹,

Sengodan²,

Sivakumar³

2017

Int J App Pharm

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, S. TAMIZHARASI, T. SIVAKUMAR Nandha College of Pharmacy and Research Institute, Erode 630052, Tamilnadu, IndiaEmail: sumoraji@gmail.comObjective: The objective of this study was to formulate and evaluate the poorly soluble drug, naringenin (NAR) into nanosuspension to increase the solubility and enhance the dissolution rate and then improve its bioavailability.Methods: Nanosuspenion of naringenin (NARNS) was prepared using high-pressure homogenization method using Soya lecithin, Polaxamer-407, Polaxamer-188, Hydroxypropyl methyl cellulose (HPMC) and Tween-80. Ten formulations were prepared to show the effect of stabilizer and its ratio. D-α-Tocopheryl polyethene glycol succinate 1000 (TPGS) was added as a co-stabilizer. All these formulations were evaluated for their particle size, PDI, zeta potential, FT-IR study, drug content, saturation solubility studies, entrapment efficiency, in vitro permeability and in vitro drug release. The formulation was further evaluated for scanning electron microscope (SEM), differential scanning calorimetry (DSC) and Powder X-ray diffraction (P-XRD) and hemocompatibility assessment.Results: All the prepared formulations were in the nano size. The optimum concentration of the stabilizer was in the formulation was found 1:1.5:1 (drug: stabilizer: co-stabilizer ratio). Dramatic effect of the particle size reduction was found by the addition of the co-stabilizer (TPGS) in formulation N2 that has P. S 80.52±0.13 nm. The solubility and dissolution of NAR in the form of NARNS were significantly higher than those of pure NAR. SEM report shows that naringenin nanosuspension revealed a smooth texture. P-XRD crystallography diffraction and DSC studies indicated that the crystalline state of NAR was converted into amorphous nature. The safety evaluation showed that NARNS provided a lower rate of erythrocyte hemolysis.Keywords: Naringenin, High-Pressure Homogenization, Nanosuspenion, Solubility, Bioavailability Conclusion:In this study, (NARNS) was successfully carried out by high-pressure homogenization technique and characterized. The physiochemical characterization shown that crystalline naringenin was converted to a polymorphic form (DSC and P-XRD Study) which evidenced by enhanced dissolution rate in comparisons of the formulation with (NAR) pure drug. The NARNS has shown 7.5±0.4 fold increased relative bioavailability when compared to the NAR. The increased drug dissolution rate may have a significant impact in absorption which in turn the improved oral bioavailability of naringenin. Thus, this delivery system may prefer to improve the dissolution of poorly soluble drugs like NAR and thus enhanced oral bioavailability. The safety evaluation showed that nanoformulation (NF2) shows a lower rate of erythrocyte hemolysis. These findings suggest that the selected formulation may represent a promising new drug formulation for intravenous administration in the treatment of certain cancers.

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Section: Introductionmentioning

confidence: 99%

Formulation and Evaluation of Polymeric Nanosuspension of Naringenin

Sumathi¹,

Sengodan²,

Sivakumar³

2017

Int J App Pharm

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“…The hydroxyl radical generated from hydrogen peroxide reacting with different transition metals is particularly damaging to DNA, leading to mutagenesis and carcinogenesis (2). Voluminous research has been carried out to discover new antioxidant compounds from plant and animal origin to prevent free radical damage (3)(4)(5)(6). Polypeptide with antioxidant property has also been reported in Chlamys farreri (7,8).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant potential of silk protein sericin against hydrogen peroxide-induced oxidative stress in skin fibroblasts

Dash

Acharya²,

Bindu³

et al. 2008

BMB Reports

175

106

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The antioxidant potential of silk protein sericin from the non-mulberry tropical tasar silkworm Antheraea mylitta cocoon has been assessed and compared with that of the mulberry silkworm, Bombyx mori. Skin fibroblast cell line (AH927) challenged with hydrogen peroxide served as the positive control for the experiment. Our results showed that the sericin obtained from tasar cocoons offers protection against oxidative stress and cell viability is restored to that of control on pre-incubation with the sericin.

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“…It is possible to gain up to a 78-fold increase in total fruit flavonols through ectopic expression of a single structural gene CHI (Verhoeyen at al., 2002).…”

Section: Engineering Of Structural Genesmentioning

confidence: 99%

Application of Plant Biotechnology Techniques in Antioxidant Production

Ivanović¹,

Milašević²,

Djurović³

et al. 2016

AgricultForest

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SUMMARYNowadays, antioxidant compounds are receiving increased attention in scholarly literature as well as in research. Antioxidants are a diverse group of compounds that can neutralize free radicals and thus help prevent diseases that are a consequence of oxidative stress. The most common antioxidant compounds are vitamins (A-carotenoids, C and E), thiols molecules (thioredoxins, glutathione), phenolic compounds (phenolic acids and flavonoids), enzymes and metal ions, as well as others. Plants have been shown to be an excellent source of antioxidant compounds, such as carotenoids, polyphenols, vitamins and betalains. Plant biotechnology uses the genetic engineering of agricultural crops as a means of producing foods rich in antioxidant nutrients, whilst plant cells and tissue culture techniques are used for the in vitro increment of antioxidant compounds in plant cells. There are numerous inspiring and promising reports about the possibilities of plant biotechnology that should provoke and encourage more research focused on antioxidant production from plants. The exogenous antioxidant molecules of important to human health (since endogenous antioxidants can be produced by the human cell itself) and the use of genetic engineering and plant cell culture techniques in antioxidant production in commonly used crops are presented in this paper.

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Increasing antioxidant levels in tomatoes through modification of the flavonoid biosynthetic pathway

Cited by 215 publications

References 23 publications

Formulation and Evaluation of Polymeric Nanosuspension of Naringenin

Formulation and Evaluation of Polymeric Nanosuspension of Naringenin

Antioxidant potential of silk protein sericin against hydrogen peroxide-induced oxidative stress in skin fibroblasts

Application of Plant Biotechnology Techniques in Antioxidant Production

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