2003
DOI: 10.1002/rcm.1210
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Increasing sensitivity and decreasing spot size using an inexpensive, removable hydrophobic coating for matrix‐assisted laser desorption/ionisation plates

Abstract: Spot size reduction and increased detection sensitivity in matrix-assisted laser desorption/ionisation (MALDI) of small molecules are accomplished by using an inexpensive and removable hydrophobic coating for MALDI targets, based on 3M Scotch Gard surface treatment. Several variations in sample preparation were explored, such as surface coating technique, identity of the matrix, solvent composition, and the type of metal support plate used. These were investigated on both uncoated and coated surfaces and their… Show more

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Cited by 47 publications
(50 citation statements)
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“…To implement this interface, the limit of detection must be improved. One way this can be accomplished is with a hydrophobic MALDI target [53,54] to obtain smaller deposited spot sizes. Under these conditions, the deposition volume can be reduced to achieve low volume fractions with a higher local concentration.…”
Section: Discussionmentioning
confidence: 99%
“…To implement this interface, the limit of detection must be improved. One way this can be accomplished is with a hydrophobic MALDI target [53,54] to obtain smaller deposited spot sizes. Under these conditions, the deposition volume can be reduced to achieve low volume fractions with a higher local concentration.…”
Section: Discussionmentioning
confidence: 99%
“…Hydrophobic target plates can also be used to improve the homogeneous sample/matrix crystallization as well as to achieve a concentration of the sample/matrix crystals onto a smaller area (Schuerenberg et al, 2000;Owen et al, 2003;van Kampen et al, 2008b). In the AnchorChip technology (Schuerenberg et al, 2000), hydrophilic anchors of 200-800 mm in diameter are placed onto a hydrophobic surface to allow more control over the size of the area onto which the sample/matrix crystals are deposited compared to hydrophobic target plates without anchors.…”
Section: B Increasing Homogeneous Sample/ Matrix Crystallizationmentioning
confidence: 99%
“…In the case of sublimed spots the matrices CHCA, SA, 4-Me-CCA, 4-HO-3-Me-CCA, and 4-HO-3-MeO-CCA were deposited by thermal sublimation in a vacuum chamber with a background pressure of about 10 Ϫ5 mbar through contact masks with 600 m diameter holes. For focusing effects with reduced spot sizes [21] the gold-sputtered aluminum oxide template surfaces were hydrophobically coated before matrix sublimation. The deposited layer thickness per spot was about 3 m. Sample preparation on sublimed spots was carried out as surface preparations with 0.5 L analyte solution using Eppendorf GELoader Tips.…”
Section: Sample Preparation and Measurementmentioning
confidence: 99%