Increasing the starch content and grain weight of common wheat by overexpression of the cytosolic AGPase large subunit gene

Kang, Guozhang; Liu, Guoqin; Peng, Xiaoqi; Wei, Liting; Wang, Chenyang; Zhu, Yunji; Ma, Ying; Jiang, Yumei; Guo, Tao

doi:10.1016/j.plaphy.2013.09.003

Cited by 82 publications

(67 citation statements)

References 30 publications

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“…Further, the development of functional markers and cloning of genes relevant to grain weight have become major research focus in the past few years. Many QTLs for grain size and weight in rice have been fine-mapped and cloned in wheat including TaCwi-1A [32], TaSus2-2B [33], TaGw2-6A [34], TaCKX6-D1 [35], TaSap1-A1 [36], TaGS1-6D [37] and TaLsu1 [38]. …”

Section: Introductionmentioning

confidence: 99%

Genome-wide association for grain morphology in synthetic hexaploid wheats using digital imaging analysis

et al. 2014

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BackgroundGrain size and shape greatly influence grain weight which ultimately enhances grain yield in wheat. Digital imaging (DI) based phenomic characterization can capture the three dimensional variation in grain size and shape than has hitherto been possible. In this study, we report the results from using digital imaging of grain size and shape to understand the relationship among different components of this trait, their contribution to enhance grain weight, and to identify genomic regions (QTLs) controlling grain morphology using genome wide association mapping with high density diversity array technology (DArT) and allele-specific markers.ResultsSignificant positive correlations were observed between grain weight and grain size measurements such as grain length (r = 0.43), width, thickness (r = 0.64) and factor from density (FFD) (r = 0.69). A total of 231 synthetic hexaploid wheats (SHWs) were grouped into five different sub-clusters by Bayesian structure analysis using unlinked DArT markers. Linkage disequilibrium (LD) decay was observed among DArT loci > 10 cM distance and approximately 28% marker pairs were in significant LD. In total, 197 loci over 60 chromosomal regions and 79 loci over 31 chromosomal regions were associated with grain morphology by genome wide analysis using general linear model (GLM) and mixed linear model (MLM) approaches, respectively. They were mainly distributed on homoeologous group 2, 3, 6 and 7 chromosomes. Twenty eight marker-trait associations (MTAs) on the D genome chromosomes 2D, 3D and 6D may carry novel alleles with potential to enhance grain weight due to the use of untapped wild accessions of Aegilops tauschii. Statistical simulations showed that favorable alleles for thousand kernel weight (TKW), grain length, width and thickness have additive genetic effects. Allelic variations for known genes controlling grain size and weight, viz. TaCwi-2A, TaSus-2B, TaCKX6-3D and TaGw2-6A, were also associated with TKW, grain width and thickness. In silico functional analysis predicted a range of biological functions for 32 DArT loci and receptor like kinase, known to affect plant development, appeared to be common protein family encoded by several loci responsible for grain size and shape.ConclusionConclusively, we demonstrated the application and integration of multiple approaches including high throughput phenotyping using DI, genome wide association studies (GWAS) and in silico functional analysis of candidate loci to analyze target traits, and identify candidate genomic regions underlying these traits. These approaches provided great opportunity to understand the breeding value of SHWs for improving grain weight and enhanced our deep understanding on molecular genetics of grain weight in wheat.

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Section: Introductionmentioning

confidence: 99%

Genome-wide association for grain morphology in synthetic hexaploid wheats using digital imaging analysis

et al. 2014

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“…Moreover, AGPase is extremely sensitive to allosteric regulation, with glycerate-3-phosphate (3-PGA) acting as an activator and Pi as an inhibitor (Slattery et al, 2000). Overexpression of the large subunit of AGPase (LSU 1) increases the starch content and grain weight of common wheat (Kang et al, 2013), while a rice mutant lacking the large subunit of ADP-glucose pyrophosphorylase in the leaf was found to have a drastically reduced starch content, but to grow normally (Tsai and Nelson, 1966). Other data reveals that the overall activity of AGPase is determined by both the large and small subunits (Dickinson and Preiss, 1969), suggesting that the involvement of both subunits should be considered when investigating N-and Pstarvation conditions.…”

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confidence: 99%

Effect of nitrogen and phosphorus deficiency on transcriptional regulation of genes encoding key enzymes of starch metabolism in duckweed ( Landoltia punctata )

Zhao

Shi

Wang

et al. 2015

Plant Physiology and Biochemistry

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“…This indicated that there was a starch formation peak during the accumulation of starch. Kang et al (2013) increased the starch content and grain weight of common wheat through overexpression of the cytosolic AGPase large subunit gene. This study hinted that AGPase is important in starch biosynthesis, and this approach could be utilized for increasing cassava starch accumulation through molecular breeding.…”

Section: The Time Course Of Cassava Root Tuber Agpase Enzyme Activitymentioning

confidence: 99%

Time Course of Starch Biosynthesis Enzymes Activity and Root Tuber Starch of Four Cassava Cultivars

Huang¹,

Luo²,

Huang³

et al. 2017

JAS

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To understand the accumulation rule of cassava root tuber starch, the amylose, amylopectin and total starch content of fresh root tuber, the enzyme activities of sucrose synthase (SuS, EC 2.4.1.13) and sucrose phosphate synthase (SPS, EC 2.4.1.14) of leaves, the enzyme activities of ADP-glucose pyrophosphorylase (AGPase, EC 2.7.7.27), soluble starch synthase (SSS, EC 2.4.1.21) and starch branching enzyme (SBE, EC. 2.4.1.18) of tubers were assessed by using cultivars SC201, SC205, GR891 and GR911 leaves and root tubers during their growth period, respectively. The results as follows: the enzyme activity of leaf SPS and the synthesis direction of SuS showed the highest at August, 2010, however the enzyme activity of the decomposing direction of SuS formed parabolic curve; the enzyme activity of tuber AGPase showed an increased then decreased single peak curve, the enzyme activity of tuber SSS oscillating decreased, while the enzyme activity of SBE was relatively stable; the amylose, amylopectin and total starch contents of fresh cassava tuber were all gradually increased along with the growth period. This research would enrich our knowledge of the time course of amylose, amylopectin and total starch contents of fresh cassava tuber, and above related enzyme activities.

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Increasing the starch content and grain weight of common wheat by overexpression of the cytosolic AGPase large subunit gene

Cited by 82 publications

References 30 publications

Genome-wide association for grain morphology in synthetic hexaploid wheats using digital imaging analysis

Genome-wide association for grain morphology in synthetic hexaploid wheats using digital imaging analysis

Effect of nitrogen and phosphorus deficiency on transcriptional regulation of genes encoding key enzymes of starch metabolism in duckweed ( Landoltia punctata )

Time Course of Starch Biosynthesis Enzymes Activity and Root Tuber Starch of Four Cassava Cultivars

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