Indigenous endophyte bacteria ability to control Ralstonia and Fusarium wilt disease on chili pepper

Abstract

Yanti Y, Warnita, Reflin, Busniah M. 2018. Indigenous endophyte bacteria ability to control Ralstonia and Fusarium wiltdisease on chili pepper. Biodiversitas 19: 1532-1538. Bacterial wilt and Fusarium wilt caused by Ralstonia syzigii subsp. indonesiensisand Fusarium oxysporum f.sp. capsici (Foc), respectively are the most damaging vascular pathogens in chili pepper (Capsicum annuumL.) and many other crops limiting their production, worldwide. Various strategies have been developed to control wilt pathogensincluding the application of chemical pesticides, which generally considered as the most effective and fastest strategy for plant diseasemanagement. However, effective chemicals for wilt pathogens of chili pepper plants are not available, yet. Endophytic bacteriaconsidered as one of options to control vascular wilt disease because of its ability to live and colonize in internal roots of plants.Previous research has been done to select endophytic indigenous bacteria isolates which can promote growth rate of chili pepper. Thepurpose of the research was to identify the selected indigenous endophyte bacteria isolates acquired from our previous study using 16SrRNA identifications and to screen the selected endophytic indigenous bacteria to control both R. syzigii subsp. indonesiensis and Foc.Results from 16S r RNA analysis showed that all of 9 isolates were identified as Bacillus spp., such as Bacillus cereus ATCC 14579,Bacillus pseudomycoides strain NBRC 101232, Bacillus toyonensis strain BCT-7112, Bacillus thuringiensis strain ATCC 10792,Bacillus weihenstephanensis strain DSM 11821, Bacillus mycoides strain 273, Bacillus cereus strain NBRC 15305, Bacillusbingmayongensis strain FJAT-13831 and Bacillus manliponensis strain BL4-6. Our results showed that most of endophytic bacteriaisolates application could control both bacterial and Fusarium wilt diseases. Six out of nine isolates can suppress R. syzigii subsp.indonesiensis without developing any symptoms and five isolates could suppress symptoms of Foc. Isolates Bacillus pseudomycoidesstrain NBRC 101232 Bacillus thuringiensis strain ATCC 10792 and Bacillus mycoides strain 273 were potential for control Foc and R.syzigii subsp. indonesiensis in chili pepper.

Short Communication: Development of selected PGPR consortium to control Ralstonia syzygii subsp. indonesiensis and promote the growth of tomatoYanti Y, Warnita, Reflin. 2018. Short Communication: Development of selected PGPR consortium to control Ralstoni

“…The most virulence bacteria (the fastest wilt disease development) was then re-cultured in TZC agar and used for plant disease infection. The pathogen was inoculated on the two weeks old plants by root wounding methods described by Yanti et al (2018a). The roots were cut in 2 sides of the plants using scissors and poured with 10 mL (10 8 CFU/mL) of the R. syzigii subsp.…”

Evaluating the potential of indigenous Rhizobacteria as biofertilizer and biopesticide against Rhizoctonia solani

“…Introduction of Bacillus spp. strains as rhizobacteria showed decrease of disease development time, incidence and severity of Ralstonia wilt diseases (Yanti et al 2018).…”

Biocontrol of Tomato Bacterial Wilt by Foliar Spray Application of a Novel Strain of Endophytic <i>Bacillus</i> sp.

“…2). The biocontrol potential of B. pseudomycoides against bacterial wilt disease has recently been reported by two research groups; Hassan et al (2017) demonstrated the biocontrol activity of rhizospheric B. pseudomycoides against bacterial wilt in tomato plants, and Yanti et al (2018) identified endophytic B. pseudomycoides from chili pepper roots as an effective BCA against the bacterial wilt of chili pepper. Although strain G4L1 was phylogenetically close to B. pseudomycoides, this strain may be distinct from this species because its colony morphology is different from that of B. pseudomycoides.…”