Indigenous Lactic Acid Bacteria from Halloumi Cheese as a Probiotics Candidate of Indonesian Origin

Abstract

This study aimed to isolate lactic acid bacteria from Halloumi cheese produced by Mazaarat Artisan cheese Indonesia, to evaluate its potential as a probiotics, and to identify it based on molecular characteristics. The lactic acid bacteria were isolated from fresh milk, curd, and the Halloumi cheese and their morphology and physiology were identified. Screening of the lactic acid bacteria isolates for probiotics candidates was mainly based on the resistance of simulated gastric juice, bile salts, antibacterial activities against pathogens, and the adhesion properties by hydrophobicity test. The selected lactic acid bacteria strains as probiotics candidates were then identified molecularly using 16S ribosomal RNA. From this study, 13 lactic acid bacteria isolates were obtained. Among the 13 isolates, K3 isolate was the most potent as a probiotics candidate. This isolate had high tolerance for simulated gastric juice and bile salts, good ability to protect the gut against pathogenic bacteria since it has antibacterial activities and had ability to adhere to human epithelial cells. Based on molecular identification, this probiotics candidate was identified as Lactobacillus plantarum.

Safety assessment of the indigenous probiotic strain <i>Lactiplantibacillus plantarum</i> subsp. <i>plantarum</i> Kita-3 using Sprague–Dawley rats as a model

“…Ratna et al . [6] reported that L. plantarum subsp. plantarum Kita-3 survives in gastric juice with a pH of 2, survives in 0.3% bile salt solution and inhibits pathogenic bacteria, such as Salmonella typhi, Escherichia coli, Shigella dysenteriae , and Staphylococcus aureu s. In addition, the results of the bacterial analysis in the colon showed that the population in the probiotic group was higher than that in the control and skim milk groups ( Table 5 ).…”

“…plantarum Kita-3 is highly resistant to simulated gastric juice and bile salts. This strain effectively adheres to human epithelial cells and protects the gut against pathogenic bacteria, such as Salmonella typhi , Escherichia coli , Shigella dysenteriae , and Staphylococcus aureus because it has antibacterial activities [6] . The use of L. plantarum subsp.…”

Dengke naniura source of probiotics to lower cholesterol

“…They came from Universitas Gajdah Mada's Center for Food and Nutrition Studies' Food and Nutrition Culture. Well diffusion methods were used to evaluate the antibacterial activity [9]. Bacteria were grown for 24 h in Muller-Hilton agar (Difco, USA).…”

“…It has been reported that strain Lactobacillus plantarum-pentosus Mut-7 and Mut-13 (isolated from gatot), Lactobacillus plantarum Dad-13 (isolated dadih), and Lb. acidophilus SNP-2 (material feses of healty infant) were able to inhibit the three pathogenic bacteria i.e Shigella dysenteriae dkt-5, Escherichia coli OK111, and E. coli ST [9].…”

“…However, in addition to producing organic acids that are antagonistic towards phatogen bacteria, also produce bacteriocin, hidrogen peroxide (H2O2), diacetyl (C4H6O2), and other secondary metabolites [3]. Lactic acid bacteria are found in various foods originating from Indonesia, as in fruit fermentation, vegetable fermentation, milk fermentation, cassava tree and glutinous rice fermentation, tempeh fermentation, moromi, and fish fermentation [4,5,6,7,8]. Prior research revealed that the LAB found in various spontaneous fermented foods in Indonesia exhibited their potential as probiotics such as Lactobacillus plantarum from gatot dadih [9].…”