Indigenous microbes and their soluble factors differentially modulate intestinal glycosylation steps in vivo

Freitas, Miguel; Axelsson, Lars-Göran; Cayuela, Chantal; Midtvedt, Tore; Trugnan, Germain

doi:10.1007/s00418-005-0004-1

Cited by 40 publications

(36 citation statements)

References 26 publications

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“…The gut mucin amount and composition are arranged based on bacterial glucodisase, activities responsible for removal of monosaccharide residues from mucin carbohydrate side chains (Hoskins et al, 1985), as well as via regulation of intestinal glycosylation (Bry et al, 1996;Bryk et al, 1999;Freitas et al, 2005;Sharma & Schumacher, 1995). An increased mucin synthesis and secretion have been shown to occur with probiotics consisting mainly of Lactobacillus and Bifidobacterium (Mack, 1999;Mack 2003).…”

Section: Discussionmentioning

confidence: 99%

Effects of in Ovo Infusion of Probiotic Strains on Performance Parameters, Jejunal Bacterial Population and Mucin Gene Expression in Broiler Chicken

Majidi-Mosleh

Sadeghi

Mousavi

et al. 2017

Rev. Bras. Cienc. Avic.

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The objective of the present study was to evaluate the effects of in ovo infusion of probiotic strains (Bacillus subtilis, Enterococcus faecium, and Pediococcus acidilactici) on jejunal microbial population and mucin gene expression in broiler chicken. In a completely randomized design, 0.5 ml of mediums containing 10 7 cfu of different probiotic strains, was administered into amniotic fluid of the 480 Cobb fetus (day 18 of incubation), with four treatments, five replicates with twenty four eggs each. For mucin gene expression, samples from the jejunum were taken on day 21 of incubation and day 3 post-hatch. Microbial profile was determined for total lactobacillus and E. coli by sampling jejunal contents on days 1 and 3 of age. Expression of the mucin gene in the jejunum was higher (p<0.05) in chicks that received Bacillus subtilis in comparison with the control group. Infusion of the probiotic strains had no effect on jejunal E. coli and lactic acid bacteria populations on day 1 post-hatch (p>0.05). There were no significant differences among treatments for performance parameters at different periods and the whole period. It was concluded that infusion of probiotic bacteria during the late of incubation has no effect on feed intake, gain and feed conversion ratio, but has a positive effect on mucin gene expression in the jejunum. The best probiotic strain for mucin gene expression was Bacillus subtilis and for beneficiary bacteria colonization was Bacillus subtilis and Pediococcus acidilactici.

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Section: Discussionmentioning

confidence: 99%

Effects of in Ovo Infusion of Probiotic Strains on Performance Parameters, Jejunal Bacterial Population and Mucin Gene Expression in Broiler Chicken

Majidi-Mosleh

Sadeghi

Mousavi

et al. 2017

Rev. Bras. Cienc. Avic.

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“…Furthermore the changes in mucin monosaccharide composition pattern as in Gal, Fuc and GalNac molar ratios need to be further assessed in terms of overall gut microbial ecology, as it is known that a correlation between mucin composition and bacterial colonization and proliferation exits. The latter is evidenced via the degradation of mucin by bacterial glycosidases (Hoskins et al, 1986;Ruas-Madiedo et al, 2008), the bacterial utilization of mucin carbohydrate side chains as energy source (Salyers, 1979;Scwad and Gä nzle, 2011;Stahl et al, 2011) and the bacterial adhesion in mucin monosaccharide (Kirjavainen et al, 1998;Gusils et al, 2003), as well as the ability of gut microflora to modulate intestinal glycosylation (Bry et al, 1996;Gheri Bryk et al, 1999;Freitas et al, 2005) and mucin gene expression (Mack et al, 1999 and. Therefore, this correlation may determine to a significant extent which species or even strains within species are best suited for utilization, colonization and/or adhesion to intestinal mucus.…”

Section: Discussionmentioning

confidence: 99%

“…Mucin monosaccharide composition depends on a dynamic interaction between adhesion of intestinal bacteria (Kirjavainen et al, 1998;Ouwehand et al, 1999;Gusils et al, 2003), host intestinal glycosylation (Bry et al, 1996;Gheri Bryk et al, 1999;Freitas et al, 2005) and microbial degradation by the intestinal microflora (Hoskins et al, 1986;Ruas-Madiedo et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Modulation of intestinal mucin composition and mucosal morphology by dietary phytogenic inclusion level in broilers

Tsirtsikos

Fegeros

Kominakis

et al. 2012

Animal

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The effect of a dietary phytogenic feed additive (PFA) inclusion level in mucin monosaccharide composition, mucosal morphometry and mucus histochemistry along the broiler intestinal tract was studied. Cobb male broilers (n 5 525) were allocated into five experimental treatments that, depending on the type of addition in the basal diet (BD), were labeled as follows: C (BD based on maize-soybean meal with no other additions), E1 (80 mg PFA/kg BD), E2 (125 mg PFA/kg BD), E3 (250 mg PFA/kg of BD) and A (2.5 mg avilamycin/kg BD). Samples from duodenum, ileum and cecum of 14-and 42-day-old broilers were collected and analyzed. In 14-day-old broilers, treatments E2 and E3 had higher (P , 0.01) duodenal mannose than treatments C, E1 and A. Ileal mannose was lower (P , 0.05) in treatment C compared with PFA treatments, and ileal galactose (Gal) was higher (P , 0.01) in treatments E2 and E3 compared with C and A. Polynomial contrast analysis with respect to PFA inclusion level showed that in 14-day-old broilers there was a linear increase (P 5 0.001) in duodenal mannose and a quadratic effect (P 5 0.038) in duodenal N-acetyl-galactosamine with increasing PFA level. Ileal Gal and mannose increased linearly (P 5 0.002 and P 5 0.012, respectively) with PFA inclusion level. There were no significant differences between treatments in mucin monosaccharide molar ratios of 42-day-old broilers. However, increasing PFA inclusion level resulted in a linear decrease of ileal fucose (P 5 0.021) and cecal N-acetylgalactosamine (P 5 0.036). Experimental treatments did not differ (P . 0.05) regarding duodenal villus height (Vh), crypt depth (Cd) and Vh/Cd ratio, irrespective of broiler age and the intestinal segment examined. However, increasing dietary PFA inclusion level showed a pattern of linear increase of duodenal Vh/Cd ratio in 14-day-old broilers and ileal Vh in 42-day-old broilers (P 5 0.039 and P 5 0.039, respectively). Alcian Blue-Periodic AcidSchiff (pH 2.5) staining of neutral and acidic mucins showed that the staining intensity of mucus layer in villi was fragment (i.e. tip, midsection and base) dependent, whereas in crypts it was dependent both on intestinal segment (i.e. duodenum, ileum and cecum) and fragment. Finally, mucus layer thickness did not differ (P . 0.05) between treatments, yet a pattern of linear increase (P , 0.05) with PFA inclusion level was observed in the duodenum of 42-day-old broilers. In conclusion, the dietary inclusion level of PFA modulated broiler intestinal mucin composition and morphology. Further studies are required to elucidate the physiological implications of such changes in host-microflora interactions.Keywords: phytogenic feed additive, essential oils, broiler, mucin, intestinal morphology ImplicationsThis study has shown that the inclusion level of a phytogenic feed additive in broiler diets has resulted in changes in the gut mucin monosaccharide composition and the intestinal mucosal architecture morphology. Given the fact that animal performance and disease prevention are prerequisite...

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“…Freitas et al (2005) have applied genetically identical germ-free mice to study the inXuence of Bacteroides thetaiotaomicron. It is not known whether the modulation of glycoconjugates is dependent upon on the presence of live bacteria or may be elicited by soluble factors produced by this bacterium.…”

Section: Histochemical Techniques In the Investigation Of Diseases Anmentioning

confidence: 99%

The histochemistry and cell biology vade mecum: a review of 2005–2006

Taatjes

Zuber

Roth

2006

Histochem Cell Biol

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The procurement of new knowledge and understanding in the ever expanding discipline of cell biology continues to advance at a breakneck pace. The progress in discerning the physiology of cells and tissues in health and disease has been driven to a large extent by the continued development of new probes and imaging techniques. The recent introduction of semi-conductor quantum dots as stable, specific markers for both fluorescence light microscopy and electron microscopy, as well as a virtual treasure-trove of new fluorescent proteins, has in conjunction with newly introduced spectral imaging systems, opened vistas into the seemingly unlimited possibilities for experimental design. Although it oftentimes proves difficult to predict what the future will hold with respect to advances in disciplines such as cell biology and histochemistry, it is facile to look back on what has already occurred. In this spirit, this review will highlight some advancements made in these areas in the past 2 years.

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Indigenous microbes and their soluble factors differentially modulate intestinal glycosylation steps in vivo

Cited by 40 publications

References 26 publications

Effects of in Ovo Infusion of Probiotic Strains on Performance Parameters, Jejunal Bacterial Population and Mucin Gene Expression in Broiler Chicken

Effects of in Ovo Infusion of Probiotic Strains on Performance Parameters, Jejunal Bacterial Population and Mucin Gene Expression in Broiler Chicken

Modulation of intestinal mucin composition and mucosal morphology by dietary phytogenic inclusion level in broilers

The histochemistry and cell biology vade mecum: a review of 2005–2006

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