Indigenous microorganisms as an effective tool for in situ bioremediation

Singh, Bishal; Christina, Evangeline

doi:10.1016/b978-0-323-89937-6.00013-9

Cited by 6 publications

(2 citation statements)

References 54 publications

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“…Particularly noteworthy is the essential role of indigenous microorganisms, which excel in their ability to generate various metabolites and their resilience to extreme conditions. This combination of attributes makes them an effective tool for optimizing the efficiency of bioremediation processes [25,26,76]. In this context, current perspectives in bioremediation are focused on identifying new microorganisms from contaminated environments [77].…”

Section: Discussionmentioning

confidence: 99%

Effect of Lyoprotective Agents on the Preservation of Survival of a Bacillus cereus Strain PBG in the Freeze-Drying Process

Farfan Pajuelo,

Carpio Mamani,

Maraza Choque

et al. 2023

Microorganisms

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Lyophilization is a widely employed long-term preservation method in which the bacterial survival rate largely depends on the cryoprotectant used. Bacillus cereus strain PBC was selected for its ability to thrive in environments contaminated with arsenic, lead, and cadmium, tolerate 500 ppm of free cyanide, and the presence of genes such as ars, cad, ppa, dap, among others, associated with the bioremediation of toxic compounds and enterotoxins (nheA, nheB, nheC). Following lyophilization, the survival rates for Mannitol 2.5%, Mannitol 10%, and Glucose 1% were 98.02%, 97.12%, and 96.30%, respectively, with the rates being lower than 95% for other sugars. However, during storage, for the same sugars, the survival rates were 78.71%, 97.12%, and 99.97%, respectively. In the cake morphology, it was found that the lyophilized morphology showed no relationship with bacterial survival rate. The best cryoprotectant for the PBC strain was 1% glucose since it maintained constant and elevated bacterial growth rates during storage, ensuring that the unique characteristics of the bacterium were preserved over time. These findings hold significant implications for research as they report a new Bacillus cereus strain with the potential to be utilized in bioremediation processes.

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Section: Discussionmentioning

confidence: 99%

Effect of Lyoprotective Agents on the Preservation of Survival of a Bacillus cereus Strain PBG in the Freeze-Drying Process

Farfan Pajuelo,

Carpio Mamani,

Maraza Choque

et al. 2023

Microorganisms

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“…It is well-known that process conditions have a strong influence on biodegradability [81,82]. In cases where biotreatment is performed on-site, there are numerous factors that may limit microbial activity and thus reduce the efficiency of biodegradation [83]. Therefore, for Delftia acidovorans and Candida parapsilosis, selected in the previous step as the bacterium and yeast with the highest potential to biodegrade PS and PVC MPs, the optimal biodegradation conditions were determined.…”

Section: Determination Of Optimal Conditions For the Biodegradation O...mentioning

confidence: 99%

Bacteria and Yeasts Isolated from the Environment in Biodegradation of PS and PVC Microplastics: Screening and Treatment Optimization

Bule Možar,

Miloloža,

Martinjak

et al. 2023

Environments

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Biodegradation is the most environmentally friendly and, at the same time, economically acceptable approach to removing various pollutants from the environment. However, its efficiency in removing microplastics (MPs) from the environment is generally low. The successful biodegradation of MPs requires microorganisms capable of producing enzymes that degrade MP polymers into compounds that the microorganisms can use as a source of carbon and energy. Therefore, scientists are screening and characterizing microorganisms that can degrade MPs more efficiently. These microorganisms are often isolated from sites contaminated with MPs because the microorganisms living there are adapted to these pollutants and should be able to better degrade MPs. In this study, five bacterial strains and five yeast strains were isolated from various environmental samples including activated sludge, compost, river sediment, and biowaste. Among them, screening was performed for bacteria and yeasts with the highest potential for the biodegradation of polystyrene (PS) and polyvinyl chloride (PVC) MPs, and the bacterium Delftia acidovorans and the yeast Candida parapsilosis were identified as the best candidates. Optimization of biodegradation of the selected MPs by each of these two microorganisms was performed, focusing on the influence of cell density, agitation speed and pH of the medium. It was found that within the selected experimental ranges, high values of cell density, low agitation speed, and a slightly basic medium favored the biodegradation of PS and PVC MPs by Delftia acidovorans. In the case of Candida parapsilosis, favorable conditions also included high cell density followed by a slightly higher, but not maximum, agitation speed and a weakly acidic medium. Broad spectroscopic and imaging methods indicated that Delftia acidovorans and Candida parapsilosis better adapt to PVC MPs to use it as a carbon and energy source.

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Unveiling the chromate stress response in the marine bacterium Bacillus enclensis AGM_Cr8: a multifaceted investigation

Monga,

Fulke,

Sonker

et al. 2024

World J Microbiol Biotechnol

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Indigenous microorganisms as an effective tool for in situ bioremediation

Cited by 6 publications

References 54 publications

Effect of Lyoprotective Agents on the Preservation of Survival of a Bacillus cereus Strain PBG in the Freeze-Drying Process

Effect of Lyoprotective Agents on the Preservation of Survival of a Bacillus cereus Strain PBG in the Freeze-Drying Process

Bacteria and Yeasts Isolated from the Environment in Biodegradation of PS and PVC Microplastics: Screening and Treatment Optimization

Unveiling the chromate stress response in the marine bacterium Bacillus enclensis AGM_Cr8: a multifaceted investigation

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