Indigenous rhizobia associated with native shrubby legumes in Taiwan

Hung, Mei-Hua; Bhagwath, Arun A.; Shen, Fo-Ting; Devasya, Rekha P.; Young, Chiu-Chung

doi:10.1016/j.pedobi.2005.06.002

Cited by 28 publications

(20 citation statements)

References 34 publications

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“…Payakapong et al (25) isolated a highly salt tolerant Ensifer species (Ensifer sp.BL3), and Hung et al (14) described strains of Rhizobium, Bradyrhizobium and Agrobacterium that could grow in a medium containing 4.5% NaCl and also grew well at 45°C. The diversity of rhizobia nodulating Medicago species has been widely reported (3,4,10,38) including in Tunisia (1,16,(44)(45)(46).…”

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confidence: 99%

Stress Tolerance and Symbiotic and Phylogenic Features of Root Nodule Bacteria Associated with Medicago Species in Different Bioclimatic Regions of Tunisia

et al. 2011

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Thirty two rhizobial isolates were obtained from different bioclimatic regions of Tunisia using as trap plants, Medicago sativa, Medicago ciliaris, Medicago polymorpha and Medicago minima. To study their diversity and characterize them in relation to Mediterranean conditions, abiotic stress resistance, symbiotic properties and genetic diversity in terms of 16S rRNA and nodA sequences were assessed. Five isolates from M. sativa, three from M. ciliaris and three from M. minima could grow at 45°C. Only two isolates from M. sativa grew at 4% NaCl. The most stress tolerant isolates were obtained from arid soils. A phylogenetic analysis of 16S rRNA genes revealed 29 isolates to be closely related to Ensifer including one (Pl.3-9) that showed a 16S rRNA sequence similar to that of Ensifer meliloti and nodA sequence similar to that of Ensifer medicae. However, three isolates were categorized into Agrobacterium containing the nodA of Ensifer. Furthermore, these isolates developed nodules on original hosts. The results for the four isolates suggest horizontal gene transfer between the species.

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confidence: 99%

Stress Tolerance and Symbiotic and Phylogenic Features of Root Nodule Bacteria Associated with Medicago Species in Different Bioclimatic Regions of Tunisia

et al. 2011

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“…The 16S rRNA gene sequence of strain CC-AFH3 T was a continuous stretch of 1453 bp. Primers 3F (59-CCTAC-GGGAGGCAGCAG-39), 5F (59-AAACTCAAATGAATTG-ACGGGG-39) and 4R (59-TTACCGCGGCTGCTGGCAC-39) were used in sequencing reactions (Hung et al, 2005).…”

Section: Cells Of Strain Cc-afh3mentioning

confidence: 99%

Description of Noviherbaspirillum malthae gen. nov., sp. nov., isolated from an oil-contaminated soil, and proposal to reclassify Herbaspirillum soli , Herbaspirillum aurantiacum , Herbaspirillum canariense and Herbaspirillum psychrotolerans as Noviherbaspirillum soli comb. nov., Noviherbaspirillum aurantiacum comb. nov., Noviherbaspirillum canariense comb. nov. and Noviher

Lin

Hameed

Arun

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

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An aerobic, Gram-negative, rod-shaped bacterium with polar flagella, strain CC-AFH3T, was isolated from an oil-contaminated site located in Kaohsiung county, Taiwan. Strain CC-AFH3T grew at 20–40 °C, pH 5.0–10.0 and <2 % (w/v) NaCl. 16S rRNA gene sequence analysis indicated that strain CC-AFH3T showed the greatest degree of similarity to Herbaspirillum soli SUEMI10T (96.5 %), H. aurantiacum SUEMI08T (96.3 %), H. canariense SUEMI03T (96.0 %), H. psychrotolerans PB1T (95.4 %) and members of other Herbaspirillum species (94.1–95.2 %), and lower similarity to members of other genera (<94 %). Phylogenetic analyses also positioned the novel strain in the genus Herbaspirillum as an independent lineage. The major fatty acids in strain CC-AFH3T were C10 : 0 3-OH, C12 : 0, C14 : 0 2-OH, C16 : 0, iso-C15 : 0 3-OH, C17 : 0 cyclo, C16 : 1ω7c/C16 : 1ω6c and C18 : 1ω7c/C18 : 1ω6c. The major polar lipids of strain CC-AFH3T were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and phosphatidylcholine. The predominant quinone was ubiquinone 8 (Q-8) and the DNA G+C content was 63.4 mol%. On the basis of 16S rRNA gene sequence analysis in combination with physiological and chemotaxonomic data, strain CC-AFH3T represents a novel species in a new genus, for which we propose the name Noviherbaspirillum malthae gen. nov., sp. nov.; the type strain of Noviherbaspirillum malthae is CC-AFH3T ( = BCRC 80516T = JCM 18414T). We also propose the reclassification of Herbaspirillum soli , Herbaspirillum aurantiacum , Herbaspirillum canariense and ‘Herbaspirillum psychrotolerans’ as Noviherbaspirillum soli comb. nov. (type strain SUEMI10T = LMG 26149T = CECT 7840T), Noviherbaspirillum aurantiacum comb. nov. (type strain SUEMI08T = LMG 26150T = CECT 7839T), Noviherbaspirillum canariense comb. nov. (type strain SUEMI03T = LMG 26151T = CECT 7838T) and Noviherbaspirillum psychrotolerans comb. nov. (type strain PB1T = DSM 26001T = LMG 27282T), respectively. An emended description of Herbaspirillum seropedicae is also presented.

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“…DNA extracted by the above methods was used as a template to amplify the 16S rRNA gene (Hung et al, 2005). PCR was performed with bacterial universal primers 1F (59-GAGTTTGATCATGGC-TCAGA-39) and 9R (59-AAGGAGGTGATCCAACCGCA-39).…”

Section: The Genus Sphingomonas Contains Environmental Isolates That mentioning

confidence: 99%

Sphingomonas formosensis sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from agricultural soil

Lin

Shen

Lai

et al. 2012

International Journal of Systematic and Evolutionary Microbiology

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In the present study, a yellow-pigmented, Gram-negative, short rod-shaped novel bacterium that was capable of degrading a wide range of polycyclic aromatic hydrocarbons (naphthalene, phenanthrene and pyrene) was isolated from agricultural soil located in Yunlin County, Taiwan. Comparative 16S rRNA gene sequence analysis positioned the novel strain in the genus Sphingomonas as an independent lineage adjacent to a subclade containing Sphingomonas fennica K101 T , Sphingomonas histidinilytica UM2 T , Sphingomonas wittichii RW1 T and Sphingomonas haloaromaticamans A175 T . 16S rRNA gene sequence analysis of strain CC-Nfb-2 T showed highest sequence similarity to S. fennica K101 T (96.2 %), S. histidinilytica UM2 T (96.1 %), S. wittichii RW1 T (95.9 %), S. haloaromaticamans A175 T (95.7 %), and Sphingobium ummariense RL-3 T (94.7 %); lower sequence similarities were observed with strains of all other Sphingomonas species. The strain contained phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, sphingoglycolipid and diphosphatidylglycerol. The predominant fatty acids were summed feature 8 (C 18 : 1 v7c and/or C 18 : 1 v6c) C 16 : 0 and 11-methyl C 18 : 1 v7c; C 14 : 0 2-OH was the major 2-hydroxy fatty acid. Previously, these lipids have been found to be characteristic of members of the genus Sphingomonas. The serine palmitoyl transferase gene (spt) was also detected and sphingolipid synthesis was confirmed. The predominant isoprenoid quinone system was ubiquinone (Q-10) and the isolate contained sym-homospermidine as the major polyamine. The DNA G+C content of the isolate was 62.8±0.8 mol%. On the basis of chemotaxonomic, phenotypic and phylogenetic data, strain CC-Nfb-2 T represents a novel species within the genus Sphingomonas, for which the name Sphingomonas formosensis sp. nov. is proposed; the type strain is CC-Nfb-2 T (5BCRC 80272 T 5DSM 24164 T ).

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Indigenous rhizobia associated with native shrubby legumes in Taiwan

Cited by 28 publications

References 34 publications

Stress Tolerance and Symbiotic and Phylogenic Features of Root Nodule Bacteria Associated with Medicago Species in Different Bioclimatic Regions of Tunisia

Stress Tolerance and Symbiotic and Phylogenic Features of Root Nodule Bacteria Associated with Medicago Species in Different Bioclimatic Regions of Tunisia

Sphingomonas formosensis sp. nov., a polycyclic aromatic hydrocarbon-degrading bacterium isolated from agricultural soil

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