Indirect effect of a turmeric diet: enhanced bile duct proliferation in Syrian hamsters with a combination of partial obstruction by Opisthorchis viverrini infection and inflammation by N-nitrosodimethylamine administration

Boonjaraspinyo, Sirintip; Boonmars, Thidarut; Aromdee, Chantana; Puapairoj, Anucha; Wu, Zhiliang

doi:10.1007/s00436-010-2031-7

Cited by 15 publications

(17 citation statements)

References 22 publications

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“…2e-h; as well as the uninfected normal control) were monitored at 30, 60, and 90 days. The gross pathology was similar to previous reports (Boonmars et al 2009a, b;Boonjaraspinyo et al 2011;Wonkchalee et al 2011). In brief, most of the gallbladders were enlarged and filled with bile fluid.…”

Section: Resultssupporting

confidence: 73%

“…Moreover, we made the novel discovery that Syrian hamsters and gerbils appear to have different components in their bile fluid. The habitat of O. viverrini in the definitive host or natural host is located in the biliary system, gallbladder, common bile duct, and hepatic bile duct (Adam et al 1993;Sripa and Kaewkes 2002;Boonmars et al 2009a, b;Boonjaraspinyo et al 2011). By histopathological observation, the present study found additional areas of O. viverrini infestation: the ampulla of Vater and the pancreatic duct (Fig.…”

Section: Discussionmentioning

confidence: 76%

“…For animal model, Syrian hamster have been used (Hutradilok et al 1983;Boonjaraspinyo et al 2011), and recently, gerbils have also been recognized (Boonmars et al 2009a, b;Wonkchalee et al 2011). However, there have been no comparative studies of optimal hosts concerning aspects such as parasite size (correlated with the size of their reproductive organs) and pathology.…”

Section: Discussionmentioning

confidence: 99%

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Comparative studies on animal models for Opisthorchis viverrini infection: host interaction through susceptibility and pathology

et al. 2011

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Syrian hamsters and gerbils are animal models for Opisthorchis viverrini infection. In both models, the parasites develop into adults with different pathologies of the hepatobiliary system. However, no comparative pathological studies have yet been completed. We therefore investigated host interaction through the susceptibility and pathological changes of Syrian hamsters and gerbils infected with 50 O. viverrini metacercariae for 30, 60, and 90 days post-infection. Animals were sacrificed at each time point for comparative study. Susceptibility and infectivity were investigated through worm burden. Parasite morphology and reproductive organs were stained with carmine and observed under light microscopy. Reproductive organs and eggs per worm were counted to confirm worm maturity. Bile acid components of both animal groups were analyzed by thin-layer chromatography. The results showed that infection in gerbils was of greater severity than in Syrian hamsters by observation of bile obstruction, enlargement of the gallbladder and common bile duct, and generation of fibrosis and cirrhosis. The worm burden of infected gerbils was lower than that observed in Syrian hamsters. Infectivity in both Syrian hamsters and gerbils was 100% with infection by 50 metacercariae; whereas with 10 metacercariae, the infectivity in gerbils was zero to very low, but still 100% in Syrian hamsters. The largest body size of worms, and the largest ovary and testes areas, was correlated with eggs per gram of feces and eggs per worm. The bile acid components cholic acid and chenodeoxycholic acid were undetectable in gerbils. The present study suggests that although Syrian hamsters, usually the host selection for an animal model, are susceptible to O. viverrini infection, infected gerbils produce worms that mature more rapidly, have larger body sizes, and more fully developed reproductive organs; this may be caused by the difference in bile acid components.

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Section: Resultssupporting

confidence: 73%

Section: Discussionmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

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Comparative studies on animal models for Opisthorchis viverrini infection: host interaction through susceptibility and pathology

et al. 2011

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“…In accordance with our previous reports (Boonmars et al 2010;Boonjaraspinyo et al 2011), pathological changes in the livers Table 1 The effect of prednisolone on histopathological changes, aggregation of inflammatory cells surrounding intrahepatic bile ducts and in liver tissue (Fig. 1a, b, g, h, m, n, s, t) and the infected group (Fig.…”

Section: Effect Of Prednisolone On the Liver Gross And Histopathologysupporting

confidence: 71%

Anti-inflammatory effect of prednisolone on the growth of human liver fluke in experimental opisthorchiasis

et al. 2011

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Opisthorchis viverrini is one of the risk factors for cholangiocarcinoma (CCA) development and is endemic in Southeast Asia including Thailand. CCA is induced by chronic inflammation from a combination of mechanical damage, parasite secretions, and immunopathology. Chronic infection with O. viverrini has been associated with several hepatobiliary diseases which affect the development of hepatobiliary cancer and CCA. Therefore, reducing the pathogenesis from O. viverrini infection may be one of the choices to reduce the risk of cholangiocarcinoma development. Prednisolone is one of the steroidal anti-inflammatory drugs that suppress inflammation, and its use has risen continuously in recent years. We therefore investigated the effect of prednisolone on pathological changes in Syrian hamster opisthorchiasis, in terms of gross and histopathological changes, worm size, eggs per gram, eggs per worm, and immunohistochemical staining for COX2. Syrian hamsters were divided into three groups: uninfected control; O. viverrini-infected (OV); and O. viverrini-infected plus prednisolone administration (OVP). The results showed an anti-inflammatory effect in the OVP group by a reduction of the inflammatory cells surrounding the intrahepatic bile ducts. However, in addition, parasite sizes for all times of observation were larger than for other groups, which was also correlated with increased eggs per worm and eggs per gram of feces. This result suggests that prednisolone is useful in suppressing inflammation in Syrian hamster opisthorchiasis, whereas it was also beneficial for parasites by enhancing their reproductive development. To clarify the mechanism of this phenomenon, further studies are under investigation.

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“…The tissue was then embedded in paraffin wax. Sections of 5 μm thickness were cut using a microtome and then stained with hematoxylin-eosin and observed under a light microscope, as in previous studies Boonjaraspinyo et al 2011). Photographs of each slide were taken at 10× and 40× magnification.…”

Section: Light Microscopic Observationmentioning

confidence: 99%

Anti-inflammatory, antioxidant and hepatoprotective effects of Thunbergia laurifolia Linn. on experimental opisthorchiasis

et al. 2012

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Thunbergia laurifolia Linn (Rang Chuet) possesses antioxidant and anti-inflammatory properties as well as anticancer activities. The aim of the present study was to evaluate the efficacy of T. laurifolia in reducing inflammation from pathological changes in Syrian hamsters infected with the human liver fluke Opisthorchis viverrini. Hamster groups were also administered N-nitrosodimethylamine (NDMA) and treated with T. laurifolia. Light microscopic observation of histopathological changes, liver function tests for alanine transaminase (ALT) and alkaline phosphatase (ALP) and kidney function tests for blood urea nitrogen (BUN) and creatinine were performed. Antioxidant effects of both fresh and dried Rang Chuet solutions were observed. Analysis of the histopathological changes showed anti-inflammatory properties, both in the case of O. viverrini infection or with NDMA administration, by reducing the aggregation of inflammatory cells surrounding the hepatic bile ducts as indicated by normal serum ALT, ALP, BUN and creatinine levels in treated Syrian hamsters. The present study found that fresh and dried Rang Chuet solutions clearly reduced the inflammatory cells in both O. viverrini-infected and NDMA-administered groups and was correlated with the total antioxidant capacity. These findings suggest that T. laurifolia possesses antioxidant and anti-inflammatory properties and that its application may be useful for prevention of the inflammatory process, one of the risk factors of O. viverrini-associated cholangiocarcinoma (CCA).

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Indirect effect of a turmeric diet: enhanced bile duct proliferation in Syrian hamsters with a combination of partial obstruction by Opisthorchis viverrini infection and inflammation by N-nitrosodimethylamine administration

Cited by 15 publications

References 22 publications

Comparative studies on animal models for Opisthorchis viverrini infection: host interaction through susceptibility and pathology

Comparative studies on animal models for Opisthorchis viverrini infection: host interaction through susceptibility and pathology

Anti-inflammatory effect of prednisolone on the growth of human liver fluke in experimental opisthorchiasis

Anti-inflammatory, antioxidant and hepatoprotective effects of Thunbergia laurifolia Linn. on experimental opisthorchiasis

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