Indirect ELISA for the Detection of Rabies Virus Antibodies in Dog Sera

Yang, Dong-Kun; Kim, Ha-Hyun; Lee, Seung Heon; Ji, Miryun; Cho, In‐Soo

doi:10.4167/jbv.2017.47.3.148

Cited by 3 publications

(7 citation statements)

References 13 publications

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“…The methods mentioned above are a bit complicated and require an ultracentrifuge. One of the most important factors for achieving high sensitivity and specificity of I-ELISA is highly purified viral antigen [15]. Therefore, we introduced a chromatographic method for purifying CAV-2 antigens for new I-ELISA.…”

Section: Discussionmentioning

confidence: 99%

“…All dog serum samples used in the VN test were diluted from 1:2 to 1:512. A VNA titer ≥ 1:2 was considered positive [7,15]. Eight sera collected from dogs were confirmed by VN test and used as positive and negative sera in optimization of I-ELISA.…”

Section: Virus Neutralization Testmentioning

confidence: 99%

“…Optimal antigen coating concentrations and dilutions of serum were determined using a checkerboard titration test, as described previously [15]. The CAV-2 antigens ranging from 50 to 0.02 µg/mL, and eight serum panel samples at dilutions of 1:20 to 40,960 were used to determine the optimal concentrations for I-ELISA.…”

Section: Establishment and Application Of I-elisamentioning

confidence: 99%

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Development of indirect ELISA for the detection of canine adenovirus type 2 antibodies in dog sera

et al. 2020

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Background: Canine adenovirus type 2 (CAV-2) induces infectious laryngotracheitis in members of the family Canidae, including dogs. To date, no ELISA kits specific for CAV-2 antibody have been commercialized for dogs in Korea. Objectives: We aimed to develop new indirect enzyme-linked immunosorbent assay (I-ELISA) to perform rapid, accurate serological surveys of CAV-2 in dog serum samples. Methods: In total, 165 serum samples were collected from dogs residing in Chungbuk and Gyeongbuk provinces between 2016 and 2018. The Korean CAV-2, named the APQA1701-40P strain, was propagated in Madin-Darby canine kidney cells and purified in an anionexchange chromatography column for use as an antigen for I-ELISA. The virus-neutralizing antibody titers of CAV-2 in the dog sera were measured by virus neutralization (VN) test. Results: We compared the results obtained between the VN and new I-ELISA tests. The sensitivity, specificity, and accuracy of new I-ELISA were 98.6%, 86.4% and 97.0% compared with VN test, respectively. New I-ELISA was significantly correlated with VN (r = 0.91). Conclusions: These results indicate that new I-ELISA is useful for sero-surveillance of CAV-2 in dog serum.

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Section: Discussionmentioning

confidence: 99%

Section: Virus Neutralization Testmentioning

confidence: 99%

Section: Establishment and Application Of I-elisamentioning

confidence: 99%

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Development of indirect ELISA for the detection of canine adenovirus type 2 antibodies in dog sera

et al. 2020

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“…The sensitivity (90.9−95.8%) of the B-ELISA for dog, raccoon dog, and cat serum compared with the FAVN test were significantly higher than those of the Platelia Rabies II Kit (80.4−88.9%) [19]. The B-ELISA was more sensitive than the I-ELISA (88.1%) based on recombinant RVN [12]. The specificity (96.5−100%) of the B-ELISA was similar to that of the Platelia Rabies II Kit (98.2−99.2%) using 0.5 IU/ml as a cutoff [19].…”

Section: Discussionmentioning

confidence: 99%

“…Previously, we expressed recombinant RABV N protein using a baculovirus system and reported an I-ELISA with moderate sensitivity (88.1%) and high specificity (92.5%) compared with the FAVN test [12]. Therefore, we need an improved ELISA with high sensitivity and specificity that can be used to test serum collected from several animal species because raccoon dogs are also responsible for transmitting rabies in Korea.…”

Section: Introductionmentioning

confidence: 99%

Development of a Blocking ELISA for Measuring Rabies Virus-specific Antibodies in Animals

Yang¹,

Kim²,

Ryu³

et al. 2018

Microbiology and Biotechnology Letters

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Development of an Indirect ELISA Featuring Plates Coated with Column Chromatographically Purified Canine Adenovirus Type-1 Antigen

et al. 2020

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Canine adenovirus type 1 (CAV-1) causes infectious hepatitis in members of the family Canidae, including dogs. An indirect enzyme-linked immunosorbent assay (I-ELISA) that detects CAV-1 antibodies is required for large-throughput tests of dog sera. We collected 165 serum samples from dogs of Chungbuk and Gyeongbuk provinces between February 2016 and October 2018. The Korean CAV-1 vaccine strain CAV1V was propagated in Madin-Darby canine kidney (MDCK) cells and purified via Nuvia cPrime anion-exchange chromatography; the virus served as an I-ELISA antigen. Virus-neutralizing anti-CAV-1 titers in dog sera were measured using the virus neutralization (VN) method. The I-ELISA was optimized using purified CAV-1 antigen and serum samples. This kit was used to evaluate dog sera. The VN and I-ELISA data were compared. The sensitivity, specificity, and accuracy of the I-ELISA were 97.0%, 74.2%, and 92.7% compared to the VN assay, respectively. The I-ELISA data significantly correlated with those of VN (r = 0.88). These results suggest that the I-ELISA is useful for serosurveillance of CAV-1 in dog sera.

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Indirect ELISA for the Detection of Rabies Virus Antibodies in Dog Sera

Cited by 3 publications

References 13 publications

Development of indirect ELISA for the detection of canine adenovirus type 2 antibodies in dog sera

Development of indirect ELISA for the detection of canine adenovirus type 2 antibodies in dog sera

Development of a Blocking ELISA for Measuring Rabies Virus-specific Antibodies in Animals

Development of an Indirect ELISA Featuring Plates Coated with Column Chromatographically Purified Canine Adenovirus Type-1 Antigen

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