1983
DOI: 10.1099/00222615-16-4-459
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Indirect enzyme-linked immunosorbent assay (Elisa) for detection of IgG antibodies against coxsackie B viruses

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Cited by 5 publications
(3 citation statements)
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“…The quality of the antigen preparation appears to have little bearing on the specificity of those tests in which the antigen is allowed to adsorb directly on to a solid phase. Even purified virus prepared by treatment with sodium lauryl sulphate to remove provirions (Brown et al, 1976) shows heterotypic activity when used under such conditions (Hannington et al, 1983). On the other hand, Katze and Crowell (1980) have shown that the type-specificity of purified coxsackie B viruses is preserved when they attach to a solid phase previously coated with specific 'capture' antibody.…”
Section: Discussionmentioning
confidence: 99%
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“…The quality of the antigen preparation appears to have little bearing on the specificity of those tests in which the antigen is allowed to adsorb directly on to a solid phase. Even purified virus prepared by treatment with sodium lauryl sulphate to remove provirions (Brown et al, 1976) shows heterotypic activity when used under such conditions (Hannington et al, 1983). On the other hand, Katze and Crowell (1980) have shown that the type-specificity of purified coxsackie B viruses is preserved when they attach to a solid phase previously coated with specific 'capture' antibody.…”
Section: Discussionmentioning
confidence: 99%
“…Stocks were prepared in continuous African green monkey kidney (VERO) cells and stored at -70°C. Purified viruses for use as antigens in ELISA, and the control antigen, were prepared as described previously (Hannington et al, 1983).…”
Section: Viruses and Antigensmentioning
confidence: 99%
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