Indirect reference intervals for haematological parameters in capillary blood of pre-school children

Balog, Zsófia Csuzdi; Dukai, Eva; Zeljković, Aleksandra; Jelić‐Ivanović, Zorana; Spasojević‐Kalimanovska, Vesna

doi:10.11613/bm.2021.010709

Cited by 8 publications

(2 citation statements)

References 23 publications

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“…Obviously, the gender effect on the concentration of erythrocyte parameters should be explored on a larger sample size. We identified visually regional variations in RBC, Hb, MCV and MCH between our data and other data reported from other cities or countries, 11,22,26–32 which probably be related to ethnicity, diet, lifestyle, living and economic conditions (Figure S1). Due to relatively small number of available research samples, the differences between the RIs in different regions did not show clear rule.…”

Section: Discussionsupporting

confidence: 70%

Reference intervals for erythrocyte parameters and hemoglobin electrophoresis parameters for young children in Guangxi

Zhou

Liu

et al. 2022

Int J Lab Hematology

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Background Erythrocyte parameter analysis is the important means for diagnosis and treatment of hematological diseases, which are essential for screening of thalassemia in southern China combined with hemoglobin electrophoresis analysis. But little is known regarding the reference intervals (RIs) in healthy pediatrics in these two areas. Methods 95% RIs of erythrocyte parameters were calculated from 853 healthy preschoolers, aged from 1 days to <6 years, according to the C28‐A3C guidelines of the Institute of Clinical and Laboratory Standards. To express the magnitude of sex and age variation, standard deviation ratio (SDR) was calculated using ANOVA. Concurrently, we selected 3814 thalassemia carriers as carriers group and drew receiver operating characteristic (ROC) curves to establish the optimal cut‐off values of hemoglobin electrophoresis parameters, which were used as the upper/lower limits of RIs to efficiently screen thalassemia. Results All parameters except red blood cell (RBC) required age partitioning, confirmed by SDRage above .4. There was no need for sex partitioning on all parameters, confirmed by SDRsex below .4. The optimal cut‐off value of Hemoglobin A2 (Hb A2) in the four subgroups was <7.8% (Hb A), 2.3%–3.2%, 2.5%–3.6% and 2.6%–3.6%, respectively. Conclusion In this study, the establishment of RIs improved the diagnostic efficiency of hematological disease (especially thalassaemia) for children in Guangxi. It provides reliable hematological references for the identification and diagnosis, treatment monitoring, and health screening of children's clinical diseases.

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Section: Discussionsupporting

confidence: 70%

Reference intervals for erythrocyte parameters and hemoglobin electrophoresis parameters for young children in Guangxi

Zhou

Liu

et al. 2022

Int J Lab Hematology

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“…Sometimes, adoption of RIs offered by the diagnostic manufacturer or by external sources could be preferred. Nonetheless, this practice might lead to errors and interpretation problems obtained in the results, especially in analytes as vitamin D, which, as mentioned above, varies cyclically over the year ( 1 , 2 , 15 ). Direct method is considered as the reference method to obtain RI, but it can be hard to organize, time-consuming and expensive.…”

Section: Introductionmentioning

confidence: 99%

Direct and indirect reference intervals of 25-hydroxyvitamin D

Fabre,

Puente-Lanzarote,

Llorente

et al. 2024

Biochem. med. (Online)

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Graphical abstract Highlights • Addressing vitamin D deficiency, we established reference intervals using direct and indirect methods • Winter reference intervals (5.4-55.7 ng/mL) contrasts with higher summer values (5.9-59.9 ng/mL), emphasizing the impact of seasons • Differences between vitamin D reference intervals obtained by direct and indirect methods were detected • The importance of regional factors is critical; notable distinctions in vitamin D values underscore the need for revised guidelines IntroductionMany studies report vitamin D (25-OH-D) deficiency, although there is no consensus among scientific societies on cut-offs and reference intervals (RI). The aim of this study is to establish and compare RI for serum 25-OH-D by direct and indirect methods. Materials and methodsTwo studies were performed in Zaragoza (Spain). A retrospective study (N = 7222) between January 2017 and April 2019 was used for RI calculation by indirect method and a prospective study (N = 312) with healthy volunteers recruited in August 2019 and February 2020 for direct method. Seasonal differences were investigated. Measurements were performed on Cobas C8000 (Roche-Diagnostics, Basel, Switzerland) using electrochemiluminescence immunoassay technology. ResultsReference intervals (2.5-97.5 percentile and corresponding 95% confidence intervals, CIs) were as follows: by indirect method 5.6 ng/mL (5.4 to 5.8) - 57.2 ng/mL (55.2 to 59.8), in winter 5.4 ng/mL (5.2 to 5.7) - 55.7 ng/mL (53.6 to 58.4), while in summer 5.9 ng/mL (5.4 to 6.2) - 59.9 ng/mL (56.3 to 62.9). By direct method 9.0 ng/mL (5.7 to 9.5) - 41.4 ng/mL (37.6 to 48.0), in winter 7.4 ng/mL (3.9 to 8.6) - 34.6 ng/mL (30.6 to 51.5), while in summer 13.3 ng/mL (10.1 to 14.1) - 44.1 ng/mL (38.9 to 66.0). In both methods, RIs were higher in summer. A significant difference was observed in 25-OH-D median values between the two methods (P < 0.001). ConclusionsReference interval calculation according to the studied area may be a useful tool to adapt the deficiency cut-offs for 25-OH-D. Our data support 25-OH-D values over 12.0 ng/mL for healthy population as sufficient, therefore current recommendations should be updated. In addition, differences in seasonality should be taken into account.

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Data Mining in Establishing the Indirect Reference Intervals of Biochemical and Haematological Assays in the Paediatric Population: A Review

Nasuruddin,

Salwana,

Sarker

et al. 2023

Lecture Notes in Computer Science

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Indirect reference intervals for haematological parameters in capillary blood of pre-school children

Cited by 8 publications

References 23 publications

Reference intervals for erythrocyte parameters and hemoglobin electrophoresis parameters for young children in Guangxi

Reference intervals for erythrocyte parameters and hemoglobin electrophoresis parameters for young children in Guangxi

Direct and indirect reference intervals of 25-hydroxyvitamin D

Data Mining in Establishing the Indirect Reference Intervals of Biochemical and Haematological Assays in the Paediatric Population: A Review

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