Indirectly assessing changes in corneal properties with OCT speckle after crosslinking in porcine eyes

Danielewska, Monika E.; Kostyszak, Malgorzata Anna; Sareło, Przemysław; Gąsior-Głogowska, Marlena; Niemczyk, Marcela; Prządka, Przemysław; Antończyk, Agnieszka; Kiełbowicz, Zdzisław; Iskander, D. Robert

doi:10.1016/j.exer.2022.109051

Cited by 4 publications

(1 citation statement)

References 28 publications

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“…Moreover, the delivery of the drug into the cornea excised from a dead animal eye can be examined using the model. The suitability of use of a dead porcine eye for this experiment was assumed from the literature review, as various model experiments with dead porcine eyes, such as a description of light propagation in the eye for understanding the eye parameters in laser treatment [45], a description of changes on a surface and cornea of a dry eye [46], a study of the widespread use of a crosslinking procedure of cornea [47], experiments on novel ways to distribute drugs with large molecules by microneedle-lens in the cornea [48], without any risk for patients, have been conducted. Similar models of dry eye with lacrimation and blinking system have been found beneficial as the experimental conditions can be fully controlled, no live animal models are needed, and the results are reproducible [49,50].…”

Section: Introductionmentioning

confidence: 99%

Controlled Drug Delivery Device for Cornea Treatment and Novel Method for Its Testing

et al. 2023

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A new solution for local anesthetic and antibiotic delivery after eye surgery is presented. A contact lens-shaped collagen drug carrier was created and loaded by Levofloxacin and Tetracaine with a riboflavin crosslinked surface layer, thus impeding diffusion. The crosslinking was confirmed by Raman spectroscopy, whereas the drug release was investigated using UV-Vis spectrometry. Due to the surface barrier, the drug gradually releases into the corneal tissue. To test the function of the carrier, a 3D printed device and a new test method for a controlled drug release, which mimics the geometry and physiological lacrimation rate of the human eye, were developed. The experimental setup with simple geometry revealed that the prepared drug delivery device can provide the prolonged release profile of the pseudo-first-order for up to 72 h. The efficiency of the drug delivery was further demonstrated using a dead porcine cornea as a drug recipient, without the need to use live animals for testing. Our drug delivery system significantly surpasses the efficiency of antibiotic and anesthetic eyedrops that would have to be applied approximately 30 times per hour to achieve the same dose as that delivered continuously by our device.

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Section: Introductionmentioning

confidence: 99%

Controlled Drug Delivery Device for Cornea Treatment and Novel Method for Its Testing

et al. 2023

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Assessing Age-Related Changes in Corneal Densitometry Parameters With Anterior Segment OCT Speckle

Fojcik,

Kościółek,

Iskander

2023

Trans. Vis. Sci. Tech.

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OCT based corneal densitometry: the confounding effect of epithelial speckle

Miażdżyk¹,

Consejo²,

Iskander³

2023

Biomed. Opt. Express

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Corneal densitometry is a clinically validated method for objectively assessing the transparency of stroma. The technique is currently dominated by Scheimpflug technology. Still, optical coherence tomography (OCT), in which examination of the statistical properties of corneal speckle is undertaken, has also been considered to assess corneal densitometry. In-vivo, the stroma is observed via the epithelium. However, the effect of this external layer on stromal densitometry has not been considered as yet. This study aims to quantify the influence of epithelium integrity on corneal OCT densitometry. OCT images from eleven freshly enucleated porcine eyes before and after epithelial debridement were used. OCT densitometry was investigated at different stromal depths using four metrics of speckle statistics. Results indicate that there exist statistically significant differences in speckle statistics for a given stromal depth depending on the presence or absence of the epithelium. The estimation error in speckle statistics can reach over 20% depending on the stromal depth. The anterior stroma densitometry values are the ones most affected by epithelial integrity. In conclusion, if OCT densitometry stromal parameters are to be considered in absolute terms, it is essential to consider the confounding effect of the epithelial layer in the analysis.

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Indirectly assessing changes in corneal properties with OCT speckle after crosslinking in porcine eyes

Cited by 4 publications

References 28 publications

Controlled Drug Delivery Device for Cornea Treatment and Novel Method for Its Testing

Controlled Drug Delivery Device for Cornea Treatment and Novel Method for Its Testing

Assessing Age-Related Changes in Corneal Densitometry Parameters With Anterior Segment OCT Speckle

OCT based corneal densitometry: the confounding effect of epithelial speckle

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