Indocyanine Green Loaded Modified Mesoporous Silica Nanoparticles as an Effective Photothermal Nanoplatform

Abstract

<jats:p>Photothermal therapy possesses great advantages for the treatment of drug-resistant tumors. Herein, Near Infrared (NIR)-triggered photothermal nanoparticles were developed through loading indocyanine green (ICG), a kind of NIR dye, into amino group-modified silica nanoparticles (SiO2-NH2 NPs). SiO2-NH2 NPs were prepared with immobilization of the amino groups into the framework of silica nanoparticles (SiO2 NPs) by employing (3-aminopropyl)-triethoxysilane (APTES). Before and after the modification of the amino group, the particle sizes of SiO2 NPs showed similar value, around 100 nm. ICG was further adsorbed into SiO2-NH2 NPs by electrostatic attraction to enable SiO2-NH2@ICG NPs as a kind of photothermal agent. The loading rate of ICG to SiO2-NH2 was greatly increased compared to unmodified SiO2, and the stability of ICG was also improved. Moreover, the SiO2-NH2@ICG NPs exhibited efficient photothermal effects due to ICG transforming laser power into local heat through the connected ICG, when NIR laser irradiation turned on for a couple of minutes. Finally, the in vitro antitumor efficacy of SiO2-NH2@ICG NPs was investigated by recording cell proliferation rate and further chronicled the apoptotic morphology evidence by a Calcein-AM/PI fluorescent staining assay, indicating the efficient photothermal targeted therapy for the HepG2 tumor cells.</jats:p>

From bone to nanoparticles: development of a novel generation of bone derived nanoparticles for image guided orthopedic regeneration

“…The red shift towards longer wavelengths is indicative of changes in the physiochemical environment, likely attributed to polaric-π* transitions resulting from electrostatic interactions between ICG molecules and the nanoparticles. 61 The florescent intensity of the ICG/BPs based on total concentration was determined using the NIR window (840 nm). Using a starting sample of 5 mg of ICG/BPs resuspended into Milli-Q water, 100uL aliquots that would equate to 40, 20, 15, 10, and 5 μM ICG (assuming a 100% loading efficiency) were measured out.…”

Recent Trends in Bio-nanomaterials and Non-invasive Combinatorial Approaches of Photothermal Therapy against Cancer

“…After surface coating of ICG, these SiO 2 -NH 2 @ICG NPs absorption in NIR-spectra progresses widely. This study revealed that these NPs exhibit significant killing of tumor cells [34]. Miranda et al illustrated that the straightforward combination of indocyanine green (ICG) with liposomes containing DOTAP (1,2-dioleoyl-3-tri-methyl-ammonium-propane) leads to complete binding of ICG to the liposomes, thereby augmenting the optical properties of ICG.…”

From Synthetic Route of Silica Nanoparticles to Theranostic Applications

“…Mesoporous silica nanoparticles are the most efficient drug delivery agent used for the codelivery of PA and chemotherapeutics in combination therapy [66]. Wang et al developed NIR-triggered photothermal nanoparticles incorporating indocyanine green into amino-group-modified silica nanoparticles [67]. They have successfully demonstrated that the synthesized photothermal nanoparticles have the potential for enhanced cancer cell killings.…”