Indole is an inter-species biofilm signal mediated by SdiA

Lee, Jintae; Jayaraman, Arul; Wood, Thomas K.

doi:10.1186/1471-2180-7-42

Cited by 411 publications

(513 citation statements)

References 65 publications

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“…2010). Several Gram‐positive and Gram‐negative bacteria synthesize indoles as intracellular signalling molecules to control the virulence of pathogenic bacteria, such as, Pseudomonas aeruginosa and enterohaemorrhagic E. coli O157:H7 (Lee et al ., 2007, 2011). In Pseudomonas putida , signalling molecules such as indole enhances TtgGHI efflux pump that are relevant for antibiotic resistance (Molina‐Santiago et al ., 2014).…”

Section: Introductionmentioning

confidence: 99%

Efficacy of 7‐benzyloxyindole and other halogenated indoles to inhibit Candida albicans biofilm and hyphal formation

Manoharan

Lee

2018

Microbial Biotechnology

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SummaryCertain pathogenic bacteria and yeast form biofilms on biotic and abiotic surfaces including medical devices and implants. Hence, the development of antibiofilm coating materials becomes relevant. The virulence of those colonizing pathogens can be reduced by inhibiting biofilm formation rather than killing pathogens using excessive amounts of antimicrobials, which is touted as one of the main reasons for the development of drug resistance. Candida albicans is an opportunistic fungal pathogen, and the transition of yeast cells to hyphal cells is believed to be a crucial virulence factor. Previous studies have shown that indole and its derivatives possess antivirulence properties against various bacterial pathogens. In this study, we used various indole derivatives to investigate biofilm‐inhibiting activity against C. albicans. Our study revealed that 7‐benzyloxyindole, 4‐fluoroindole and 5‐iodoindole effectively inhibited biofilm formation compared to the antifungal agent fluconazole. Particularly, 7‐benzyloxyindole at 0.02 mM (4.5 μg ml−1) significantly reduced C. albicans biofilm formation, but had no effect on planktonic cells, and this finding was confirmed by a 2,3‐bis‐(2‐methoxy‐4‐nitro‐5‐sulfophenyl)‐2H‐tetrazolium‐5‐carboxanilide (XTT) assay and three‐dimensional confocal laser scanning microscopy. Scanning electron microscopy analyses revealed that 7‐benzyloxyindole effectively inhibited hyphal formation, which explains biofilm inhibition. Transcriptomic analysis showed that 7‐benzyloxyindole downregulated the expressions of several hypha/biofilm‐related genes (ALS3,ECE1,HWP1 and RBT1). A C. albicans‐infected Caenorhabditis elegans model system was used to confirm the antivirulence efficacy of 7‐benzyloxyindole.

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Section: Introductionmentioning

confidence: 99%

Efficacy of 7‐benzyloxyindole and other halogenated indoles to inhibit Candida albicans biofilm and hyphal formation

Manoharan

Lee

2018

Microbial Biotechnology

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“…S. typhimurium and E. coli have a luxR homolog called sdiA, but do not produce any detectable AHLs (Wang et al, 1991;Smith and Ahmer, 2003). Importantly, SdiA has been shown to respond to exogenous AHLs and regulate virulence in S. typhimurium and biofilm formation in E. coli (Wang et al, 1991;Smith and Ahmer, 2003;Lee et al, 2007); both phenotypes are important for competition and host interactions.…”

Section: Incomplete Qs Circuitsmentioning

confidence: 99%

“…For example, Pseudomonas aeruginosa contains two QS circuits (LasI/R and RhlI/R) but also contains QscR and a fourth LuxR-type protein identified here ( Figure 1b). It has been suggested that these extra LuxR-type proteins are orphans (Fuqua, 2006); however, as they belong to a characterized protein family (AHL response regulators) and the biological role of several has been described (Wang et al, 1991;Smith and Ahmer, 2003; Lee et al, 2006Lee et al, , 2007Lequette et al, 2006), they cannot be considered orphans. The best-characterized of these extra LuxR-type proteins in a bacterium with complete QS circuits is BisR in Rhizobium leguminosarum bv.…”

Section: Extra Luxr-type Proteinsmentioning

confidence: 99%

AHL-driven quorum-sensing circuits: their frequency and function among the Proteobacteria

2008

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“…One recently-characterized metabolite biofilm signal is indole, which represses biofilm formation of E. coli by repressing motility, inducing the sensor of the quorum-sensing signal autoinducer-1 and influencing acid resistance (Lee et al, 2007b). A series of genes including bssR/bssS, bhsA and ariR have been identified as regulating biofilm formation through indole (Domka et al, 2006;Lee et al, 2007c;Zhang et al, 2007), and hydroxy indoles have been shown to be signals for pathogenic E. coli Lee et al, 2007a).…”

Section: Introductionmentioning

confidence: 99%

Escherichia coli transcription factor YncC (McbR) regulates colanic acid and biofilm formation by repressing expression of periplasmic protein YbiM (McbA)

2008

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Quorum-sensing signal autoinducer 2 (AI-2) stimulates Escherichia coli biofilm formation through the motility regulator MqsR that induces expression of the putative transcription factor encoded by yncC. Here, we show that YncC increases biofilm formation by repressing overproduction of the exopolysaccharide identified as colanic acid (corroborated by decreasing mucoidy and increased sensitivity to bacteriophage P1 infection). Differential gene expression and gel shift assays demonstrated that YncC is a repressor of the predicted periplasmic protein-encoding gene, ybiM, which was corroborated by the isogenic yncC ybiM double mutation that repressed the yncC phenotypes (biofilm formation, colanic acid overproduction, mucoidy and bacteriophage resistance). Through nickel-enrichment DNA microarrays and additional gel shift assays, we found that the putative transcription factor B3023 (directly upstream of mqsR) binds the yncC promoter. Overexpressing MqsR, AI-2 import regulators LsrR/LsrK and AI-2 exporter TqsA induced yncC transcription, whereas the AI-2 synthase LuxS and B3023 repressed yncC. MqsR has a toxic effect on E. coli bacterial growth, which is partially reduced by the b3023 mutation. Therefore, AI-2 quorum-sensing control of biofilm formation is mediated through regulator MqsR that induces expression of the transcription factor YncC. YncC inhibits the expression of periplasmic YbiM, which prevents overproduction of colanic acid (excess colanic acid causes mucoidy) and prevents YbiM from inhibiting biofilm formation.

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Indole is an inter-species biofilm signal mediated by SdiA

Cited by 411 publications

References 65 publications

Efficacy of 7‐benzyloxyindole and other halogenated indoles to inhibit Candida albicans biofilm and hyphal formation

Efficacy of 7‐benzyloxyindole and other halogenated indoles to inhibit Candida albicans biofilm and hyphal formation

AHL-driven quorum-sensing circuits: their frequency and function among the Proteobacteria

Escherichia coli transcription factor YncC (McbR) regulates colanic acid and biofilm formation by repressing expression of periplasmic protein YbiM (McbA)

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