Indução de embriogênese somática em cupuaçu (Theobroma grandiflorum Schum.)

Ferreira, M. das G. R.; Carvalho, C. H. S.; Carneiro, Andréia de Almeida; Filho, Carlos Ferreira Damião

doi:10.1590/s0100-29452005000300038

Cited by 5 publications

(9 citation statements)

References 12 publications

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“…The data presented here demonstrate that the reaction to acetocarmine was identical for both growth regulators; however, the 2,4-D-treated cells were less reactive to Evans blue, particularly at the concentration of 4.5 μM. According to Steiner et al (2005) and George et al (2008), a positive reaction to acetocarmine versus Evans blue identifies embryogenic cells. Thus, the J. curcas callus grown on 4.52 μM of 2,4-D exhibited a higher embryogenic potential.…”

Section: Resultssupporting

confidence: 60%

“…The SEM analysis showed that the cell masses treated with 4.52 μM of 2,4-D were rounded and that structures similar to embryoid calogênica appeared between the surfaces (Figure 1 F and G). These structures were also observed by Ferreira et al (2005) to induce somatic embryogenesis in cupuaçu (Theobroma grandiflorum) under the effect of TDZ (Thidiazuron). The developmental stages of somatic embryos include globular, heart, torpedo and cotyledon stages.…”

Section: Resultsmentioning

confidence: 73%

“…The percent coverage of the explants by callus (% CEC) was evaluated at 30 and 60 days, and a grade of 0 (zero), 1 (a) or 2 (two) was assigned to each explant, representing 0, 50 and 100%, respectively, callus coverage of the explant. The Scott-Knott test was used to assess the between-group differences (FERREIRA, 2011 A cytochemical analysis was performed using a fraction of the reclaimed material. For this purpose, fractions were collected from 100 mg of callus tissue from each treatment, and the material was subjected to double acetocarmine / Evans blue staining according to the methodology described by Steiner et al (2005).…”

Section: Methodsmentioning

confidence: 99%

“…The most widely used hormone in callus induction is the auxin 2,4-D (2,4-dichlorophenoxyacetic acid). According to George et al (2008), 2,4-D is the most commonly used hormone in the regulation of embryogenic callus induction, although picloram (4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid) is also widely used (PERÁN-QUESADA et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Some callus cells are small and isodiametric with a dense cytoplasm, these cells are more reactive to acetocarmine, staining a red color (GEORGE et al, 2008), and are considered embryogenic (STEINER et al, 2005). Other callus cells are elongated due to high vacuolation and are considered non-embryogenic: these cells are permeable to Evans blue and, therefore, stain blue (SUAREZ et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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<b>Ultrastructural and cytochemical analysis of physic nut callus tissue in response to different combinations of growth regulators

et al. 2015

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ABSTRACT. This study aimed to induce callus formation in Jatropha curcas L. and to evaluate the ultrastructure and cytochemical behavior of the calli. Calluses were induced with 2,4-D, picloram-PIC, kinetin (Kin) and BAP: (1) control; (2) 4.52 μM 2,4-D; (3) 9.04 μM 2,4-D; (4) 4.14 μM PIC; (5) 8.28 μM PIC; (6) 4.52 μM 2,4-D + 2.32 μM KIN; (7) 9.04 μM 2,4-D + 4.64 μM KIN; (8) 4.14 μM PIC + 2.32 μM KIN; (9) 8.28 μM PIC + 4.64 μM KIN; (10) 4.52 μM 2,4-D + 2.22 μM BAP; (11) 9.04 μM 2,4-D + 4.44 μM BAP; (12) 4.14 μM PIC + 2.22 μM BAP and (13) 8.28 μM PIC + 4.44 μM BAP. It was evaluated the percent coverage of the explants by callus (% CEC) and performed scanning electron microscopy (SEM) and acetocarmine/Evans blue double staining to analyze the embryogenic potential of the calli. As shown by scanning electron microscopy and acetocarmine/Evans blue staining, we found that J. curcas callus formation was optimal with 4.52 μM of 2,4-D.Keywords: Jatropha curcas L., embryogenesis, microscopy, cytochemistry. Ultraestrutura e citoquímica de calos de pinhão-manso em resposta a balanços entre fitorreguladores RESUMO. Objetivou-se induzir a formação de calos em Jatropha curcas L., avaliar a ultraestrutura e o comportamento citoquímico dos calos. Os calos foram induzidos com 2,4-D, picloran, cinetina e BAP: (1) testemunha; (2) 4,52 μM 2,4-D; (3) 9,04 μM 2,4-D; (4) 4,14 μM PIC; (5) 8,28 μM PIC; (6) 4,52 μM 2,4-D + 2,32 μM KIN; (7) 9,04 μM 2,4-D + 4,64 μM KIN; (8) 4,14 μM PIC + 2,32 μM KIN; (9) 8,28 μM PIC + 4,64 μM KIN; (10) 4,52 μM 2,4-D + 2,22 μM BAP; (11) 9,04 μM 2,4-D + 4,44 μM BAP; (12) 4,14 μM PIC + 2,22 μM BAP and (13) 8,28 μM PIC + 4,44 μM BAP. Foi avaliado a porcentagem de cobertura dos explantes por calos (%CEC), microscopia eletrônica de varredura (MEV) e dupla coloração com carmim acético/azul de evans. A calogênese é otimizada em meio MS acrescido de 4,52 μM de 2,4-D, condição em que pela análise de microscopia eletrônica de varredura e dupla coloração carmim acético/azul de evans há expressão de potencial embriogênico nos calos de J. curcas.

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Section: Resultssupporting

confidence: 60%

Section: Resultsmentioning

confidence: 73%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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<b>Ultrastructural and cytochemical analysis of physic nut callus tissue in response to different combinations of growth regulators

et al. 2015

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Gene expression and morphological characterization of cell suspensions of Coffea arabica L. cv. Catiguá MG2 in different cultivation stages

et al. 2015

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The present study aimed to evaluate the quality of cell suspension of Coffea arabica cv. Catiguá at four different ages by morphological and gene expression analysis. For this purpose, cell suspension samples were collected at 30, 45, 60 and 75 days. Morphological analysis revealed the presence of embryogenic regions in all samples, and after 60 days, non-embryogenic regions were also identified. The genic analyses revealed that as the cells were transferred to a new medium a change in both their physical and chemical conditions was noticed that caused stress. The decrease in SERK and BBM genes expression after 75th day may also be due to the non-embryogenic regions, characterized by large, elongated and vacuolated cells that were observed in the periphery of embryogenic regions, starting from 60 days of culture.

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Avaliações morfofisiológicas do desenvolvimento de microplantas de pupunheiras submetidas a tratamentos com biorreguladores

Graner¹

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This study aimed to evaluate the role of bioregulators (NAA, BAP, TDZ and 2iP) on the morphogenetic development of pejibaye microplants. For this, every 28 days, the number of propagules, the root development, the length and the growth rate of shoots, the number of leaves, the oxidation, the number of meristematic centers in the explants basal region and in the leaf sheaths and/or in the propagules leaf primordia, the number of pro-embryogenic cell complex and the morphogenic routes and patterns were measured. All experiments were conducted in a randomized design. Qualitative data were presented as percentages and general averages, and quantitative data were subjected to polynomial regression or exponential analysis. The morphophysiological evaluations showed that the TDZ alone favored the development of adventitious buds, although short, and sometimes with thorns. Only in the treatments with the isolated presence of BAP, TDZ and 2iP, somatic embryos were not developed. For the other treatments there was an exponential behavior, in which the number of somatic embryos was reduced and varied according only to the cultivation period. Although a greater number of roots was observed in the treatment, in which NAA was associated with BAP, the control treatment has induced the highest rate of rooted explants and with greater root length, and has been the single one that produced branches. The isolated presence of TDZ promoted the greater shoot length, and its association with ANA promoted the highest growth rate. The best shoot development of pejibaye microplants and explants occurred in the MS bioregulator-free culture medium of or in the medium with NAA/TDZ association, at140 days of cultivation. Moreover, the isolated addition of the cytokinins BAP, TDZ and 2iP in the medium, later promoted the highest growth rate. In pejibaye explants the addition of 2iP alone promoted intense oxidation of the first developed leaves and the application of TDZ alone or associated with ANA promoted anomalies such as folding leaf and thorn development. The monitoring of the morphogenetic routes and patterns showed that in the treatments that were bioregulator-free, or with the isolated presence of NAA and TDZ, or the association of both, somatic embryos, from multicellular origin, raised up directly in the central region of the explants base. Pro-embryos from unicellular origin were also induced in the isolated presence of NAA or in NAA/BAP and NAA/TDZ associations. Moreover, in the isolated presence of TDZ, pro-embryos from multicellular origin were induced from shoot apical meristem activity and from the (bi) polarization of a large number of meristematic centers in the distal extremity of the explants base. Adventitious buds with multicellular origin from the proximal extremity of the explants base were induced more frequently in the bioregulator-free treatment and in the treatment with NAA/TDZ. Between the leaf explants primordia, adventitious buds were often induced by the treatments with the isolated presence of ANA.

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Indução de embriogênese somática em cupuaçu (Theobroma grandiflorum Schum.)

Cited by 5 publications

References 12 publications

<b>Ultrastructural and cytochemical analysis of physic nut callus tissue in response to different combinations of growth regulators

<b>Ultrastructural and cytochemical analysis of physic nut callus tissue in response to different combinations of growth regulators

Gene expression and morphological characterization of cell suspensions of Coffea arabica L. cv. Catiguá MG2 in different cultivation stages

Avaliações morfofisiológicas do desenvolvimento de microplantas de pupunheiras submetidas a tratamentos com biorreguladores

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