Indução de obesidade com sacarose em ratos

Abstract

-Background -Although is complex to identify the factors responsible for the important growth in obesity all over the world, the main causes are increased consumption of energy, highly saturated fats and sugars, and reduced physical activity. Aim -To compare rats with normal and supplemented diet with sucrose in relationship to body mass, weight of gonadal and retroperitoneal fat and Lee index. MethodsForty rats were divided into two groups: 20 in the control group that received normal chow diet and water for three months, and 20 animals in the experimental group who received the same diet but supplemented with sucrose 300 g/l of water. The animals were weighed once a week during 91 days. At scheduled death, they had measured the naso-anal length, body weight and Lee index. After laparotomy, retroperitoneal and gonadal fat were isolated, dried and the percentage of weight in relation to body weight at the date of death was evaluated. Results -There was a statistic significant difference between the 14 th and 78 th day favoring the experiment group indicating that sucrose interferes with weight gain in rats. The average weight was higher in the experimental group in all periods in comparison to initial weight. There was also significant difference in the weight of the gonadal and retroperitoneal fat. There was no significant difference comparing the Lee index. Conclusion -The body mass index was higher in animals treated with diet supplemented with sucrose and had higher gonadal and retroperitoneal fat, but no difference in the Lee index.RESUMO -Racional -Embora seja muito complexo identificar o que levou ao grande crescimento da obesidade, as causas principais são o aumento do consumo de alimentos energéticos e ricos em gorduras saturadas e açucares, e a redução das atividades físicas. Objetivos -Comparar a massa corpórea, o peso da gordura retroperitoneal e gonadal e o índice de Lee em ratos submetidos à dieta normal e à suplementada com sacarose. Métodos -A amostra foi de 40 animais, divididos em dois grupos: 20 do grupo controle que receberam dieta de ração normal e água por três meses e 20 do grupo experimento que receberam a mesma ração suplementada com sacarose 300 g/l na água. Os animais foram pesados uma vez por semana durante 91 dias. Na data da morte foram aferidos o comprimento nasoanal, peso corporal e foi calculado o índice de Lee. Após, foram submetidos à laparotomia e a gordura retroperitoneal e gonadal foram individualizadas, ressecadas e avaliada a porcentagem do peso dela em relação ao corporal na data da morte. Resultados -Verificou-se diferença estatisticamente significativa entre o 14º até o 78º dia, quando comparados os grupos indicando que a sacarose interfere no ganho de peso dos ratos. A média de peso foi maior no grupo experimento em todos os períodos, tendo como referência o peso inicial. Verificou-se diferença significativa a favor do grupo experimento no peso da gordura gonadal e retroperitoneal. Não houve diferença significativa da comparação do índice de Lee. Conclusão -A ...

Intermittent Fasting for Twelve Weeks Leads to Increases in Fat Mass and Hyperinsulinemia in Young Female Wistar Rats

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Long-term sucrose solution consumption causes metabolic alterations and affects hepatic oxidative stress in wistar rats

“…Furthermore, sucrose ingestion diminished feed efficiency, suggesting that sucrose metabolism alters processes involved in the regulation of body weight and exhibits adverse nutritional effects. Morphometric parameters were not influenced by sucrose ingestion, similar to what was reported by Malafaia et al (2013). 6 months after receiving the sucrose solution, rats exhibited similar glycemia.…”

“…The hormone insulin is necessary for fat metabolism and both lipogenesis and lipolysis are critical pathways involved in balancing adipose tissue lipid content. The sucrose group showed marked elevation of fat deposition and adiposity index, supporting the earlier observations by Malafaia et al (2013). Increased levels of glucose and fructose serve as acetyl-CoA sources for conversion to fatty acids for storage in hepatic and adipose tissues, thus contributing to NAFLD and obesity, respectively (Sanders and Griffin, 2016).…”

Predictive behaviors for anxiety and depression in female Wistar rats subjected to cafeteria diet and stress

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