Induced albumin secretion from HepG2 spheroids prepared using poly(ethylene glycol) derivative with oleyl groups

Taguchi, Tetsushi; Rao, Zihe; Ito, Michiko; Matsuda, Miyuki

doi:10.1007/s10856-011-4414-4

Cited by 14 publications

(15 citation statements)

References 30 publications

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“…In addition, we confirm that HepG2 cells, a commonly used hepatocyte cell line, benefit from aggregation and three dimensional cell culture and that their function is increased in the larger aggregates used in this study. This is in line and complementary with studies reporting differentiation and increased expression of several hepatocyte specific markers (such as increased albumin secretion, urea, …) in HepG2 spheroids [7], [17]…”

Section: Discussionsupporting

confidence: 89%

High Throughput Micro-Well Generation of Hepatocyte Micro-Aggregates for Tissue Engineering

et al. 2014

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The main challenge in hepatic tissue engineering is the fast dedifferentiation of primary hepatocytes in vitro. One successful approach to maintain hepatocyte phenotype on the longer term is the cultivation of cells as aggregates. This paper demonstrates the use of an agarose micro-well chip for the high throughput generation of hepatocyte aggregates, uniform in size. In our study we observed that aggregation of hepatocytes had a beneficial effect on the expression of certain hepatocyte specific markers. Moreover we observed that the beneficial effect was dependent on the aggregate dimensions, indicating that aggregate parameters should be carefully considered. In a second part of the study, the selected aggregates were immobilized by encapsulation in methacrylamide-modified gelatin. Phenotype evaluations revealed that a stable hepatocyte phenotype could be maintained during 21 days when encapsulated in the hydrogel. In conclusion we have demonstrated the beneficial use of micro-well chips for hepatocyte aggregation and the size-dependent effects on hepatocyte phenotype. We also pointed out that methacrylamide-modified gelatin is suitable for the encapsulation of these aggregates.

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Section: Discussionsupporting

confidence: 89%

High Throughput Micro-Well Generation of Hepatocyte Micro-Aggregates for Tissue Engineering

et al. 2014

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“…• Use cell culture medium additives (e.g., carboxymethyl cellulose, methylcellulose) (see Table 4) [18,38,82,84,87,88,[105][106][107] Various spheroids are formed on the non-adherent surface, i.e., spheroids are not produced individually…”

Section: Cell Culture Medium Additives That Are Used To Improve Cellumentioning

confidence: 99%

Spheroids Formation on Non‐Adhesive Surfaces by Liquid Overlay Technique: Considerations and Practical Approaches

Costa

Melo‐Diogo

Moreira

et al. 2017

Biotechnology Journal

155

139

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Scalable and reproducible production of 3D cellular spheroids is highly demanded, by pharmaceutical companies, for drug screening purposes during the pre-clinical evaluation phase. These 3D cellular constructs, unlike the monolayer culture of cells, can mimic different features of human tissues, including cellular organization, cell-cell and cell-extracellular matrix (ECM) interactions. Up to now, different techniques (scaffold-based and -free) have been used for spheroids formation, being the Liquid Overlay Technique (LOT) one of the most explored methodologies, due to its low cost and easy handling. Additionally, during the last few decades, this technique has been widely investigated in order to enhance its potential for being applied in high-throughput analysis. Herein, an overview of the LOT advances, practical approaches, and troubleshooting is provided for those researchers that intend to produce spheroids using LOT, for drug screening purposes. Moreover, the advantages of the LOT over the other scaffold-free techniques used for the spheroids formation are also addressed. Highlights • 2D cell culture drawbacks are summarized;• spheroids mimic the features of human tissues;• scaffold-based and scaffold-free technologies for spheroids production are discussed; • advantages of LOT over other scaffold-free techniques are highlighted; • LOT advances, practical approaches and troubleshooting are underlined.

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“…Cell assembly was confirmed when 69Chol-T-Gltn was added to the cells, and firm cell assembly was observed after culture for 1 day when the concentration of 69Chol-T-Gltn was 10%. In our previous study, HepG2 cell assembly was confirmed when cells were cultured with di-oleoyl group-terminated poly (ethylene) glycol (Ole-PEG-Ole) [24]. This Ole-PEG-Ole can also play a similar role as Chol-T-Gltns, that is, hydrophobic groups such as Ole or Chol groups anchor to the cell membrane, resulting in cell assembly.…”

Section: Resultsmentioning

confidence: 98%

“…The RGD sequence of 69Chol-T-Gltn also worked as a scaffold for cell adhesion and proliferation. We previously added Ole-PEG-Ole to HepG2 cells with different concentrations [24]. However, the number of HepG2 cells decreased with increasing concentration of Ole-PEG-Ole, even though cell function was promoted.…”

Section: Resultsmentioning

confidence: 99%

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Crosslinking Liposomes/Cells Using Cholesteryl Group-Modified Tilapia Gelatin

Taguchi

Endo

2014

IJMS

Self Cite

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Cholesteryl group-modified tilapia gelatins (Chol-T-Gltns) with various Chol contents from 3 to 69 mol % per amino group of Gltn were prepared for the assembly of liposomes and cells. Liposomes were physically crosslinked by anchoring Chol groups of Chol-T-Gltns into lipid membranes. The resulting liposome gels were enzymatically degraded by addition of collagenase. Liposome gels prepared using Chol-T-Gltn with high Chol content (69Chol-T-Gltn) showed slower enzymatic degradation when compared with gels prepared using Chol-T-Gltn with low Chol content (3Chol-T-Gltn). The hepatocyte cell line HepG2 showed good assembly properties and no cytotoxic effects after addition of 69Chol-T-Gltns. In addition, the number of HepG2 cells increased with concentration of 69Chol-T-Gltns. Therefore, Chol-T-Gltn, particularly, 69Chol-T-Gltn, can be used as an assembling material for liposomes and various cell types. The resulting organization can be applied to various biomedical fields, such as drug delivery systems, tissue engineering and regenerative medicine.

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Induced albumin secretion from HepG2 spheroids prepared using poly(ethylene glycol) derivative with oleyl groups

Cited by 14 publications

References 30 publications

High Throughput Micro-Well Generation of Hepatocyte Micro-Aggregates for Tissue Engineering

High Throughput Micro-Well Generation of Hepatocyte Micro-Aggregates for Tissue Engineering

Spheroids Formation on Non‐Adhesive Surfaces by Liquid Overlay Technique: Considerations and Practical Approaches

Crosslinking Liposomes/Cells Using Cholesteryl Group-Modified Tilapia Gelatin

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