Induced Cell Death as a Possible Pathway of Antimutagenic Action

Еремина, Н. В.; Жанатаев, А. К.; Дурнев, А. Д.

doi:10.1007/s10517-021-05161-z

Cited by 3 publications

(4 citation statements)

References 143 publications

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“…Green tea leaves contain catechins, tannins, various vitamins, and other bioactive substances. In particular, it is known that extracts of green tea leaves, like those of other plants, contain polyphenols (for example, epigallocatechin gallate) [12] that at certain concentrations can induce cell apoptosis through the initiation of a caspase-3-dependent pathway, which determines their antimutagenic impact [4]. Persimmon fruits contain vitamins C and B, trace elements zinc, copper, iron, chromium, etc.…”

Section: Resultsmentioning

confidence: 99%

“…Thus, phytocomposition of green tea leaves and Caucasian persimmon fruit extracts exhibits concentration-dependent antimutagenic properties in studies on peripheral blood lymphocytes exposed to alkylating compounds (NMU and SF). As antimutagens under certain conditions -depending on doses and concentrations -can exhibit co-mutagenic effects [4], phytocompositions with the expected antimutagenic effect should be pre-tested to select the most effective concentrations/doses for specific mutagens.…”

Section: Resultsmentioning

confidence: 99%

“…Cytogenetic analysis was performed on cultures of peripheral blood lymphocytes, and the frequency of aberrant metaphases was analyzed [4]. It should be noted that studies carried out on human peripheral lymphocytes are mandatory for broad introduction of the studied phytocomposites.…”

Section: Methodsmentioning

confidence: 99%

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Antimutagenic Effects of the Composition from Green Tea Leaves Extracts and Caucasian Persimmon Fruits

Huseynov

Абдуллаев

2021

Bull Exp Biol Med

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On a culture of human peripheral blood lymphocytes, antimutagenic activity of a composition from extracts of green tea leaves and Caucasian persimmon fruits was established with a modification of the mutation process induced by chemical compounds producing an alkylating effect (nitrosomethylurea and sodium fluoride). A concentration dependence of the antimutagenic efficiency of the studied phytocomposite was shown. The highest antimutagenic efficiency was observed when a combination of green tea extract at a concentration of 0.01 μg/ml and persimmon fruit extract at a concentration of 0.001 μg/ml were used. Moreover, this combination was most effective against mutations induced by both nitrosomethylurea and sodium fluoride: the antimutagen efficiency factor was 0.53 and 0.55, respectively.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Antimutagenic Effects of the Composition from Green Tea Leaves Extracts and Caucasian Persimmon Fruits

Huseynov

Абдуллаев

2021

Bull Exp Biol Med

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“…Selected by its desirable characteristics (high Q3G content, antioxidant activity and partition coefficient), the hydrolyzed rutin 01 protected CHO-K1 cells from the MMS-induced mutagenic effect similar to quercetin and rutin. Although it seems contradictory, mutagenic effects that result in the activation of regulated cell death mechanisms have been described as a mechanism of anti-mutagenic activity of polyphenols [ 25 ], along with the ROS scavenging ability and activation of DNA repair mechanisms [ 1 , 2 ]. According to the literature, the mutagenic and anti-mutagenic effects of rutin, Q3G and quercetin have already been demonstrated in different experimental models.…”

Section: Discussionmentioning

confidence: 99%

Enzymatic Hydrolysis of Rutin: Evaluation of Kinetic Parameters and Anti-Proliferative, Mutagenic and Anti-Mutagenic Effects

Sobreiro

Torre

Araújo

et al. 2023

Life

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The bioavailability of glucoside flavonoids is influenced by the nature of the sugar, glucosides being absorbed faster than rhamnoglucosides, for example. One strategy to enhance the bioavailability is enzymatic hydrolysis. In this study, some kinetic parameters of hesperidinase-mediated hydrolysis of rutin were evaluated using an UHPLC/QTOF-MSE analysis of the products of a bioconversion reaction. The resulting hydrolyzed rutins (after 4, 8 and 12 h of reaction) were submitted to anti-proliferative and Cytokinesis-Block Micronucleus (CBMN) assays in CHO-K1 cells. In the hesperidinase-mediated hydrolysis, the final concentration of quercetin-3-O-glucoside (Q3G) was directly proportional to the rutin concentration and inversely proportional to the reaction time. At an anti-proliferative concentration (2.5 μg/mL), hydrolyzed rutin derivatives did not show a mutagenic effect, except for the sample with a higher content of Q3G (after 4 h of the enzymatic hydrolysis of rutin). Moreover, the higher Q3G content in hydrolyzed rutin protected the CHO-K1 cells 92% of the time against methyl methanesulfonate-induced mutagenic damage. These results suggested that the anti-mutagenic effect of hydrolyzed rutin might be related to antioxidant and cell death induction. Presenting a good lipophilicity/hydrophilicity ratio, together with antioxidant and anti-mutagenic activities, the hesperidinase-mediated hydrolyzed rutin seemed to be a promisor raw material for the development of food supplements.

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Greener Stability-Indicating HPLC Approach for the Determination of Curcumin in In-House Developed Nanoemulsion and Curcuma longa L. Extract

et al. 2023

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Despite the fact that several analytical methodologies have been reported for the determination of curcumin (CCM) in a wide range of sample matrices, the greener liquid chromatographic approaches to determine CCM are scarce in the literature. Therefore, this research is designed to develop and validate a greener stability-indicating “high-performance liquid chromatography (HPLC)” methodology to determine CCM in an in-house developed nanoemulsion, Curcuma longa L. extract, and commercial tablets. CCM was measured on a Nucleodur (150 mm × 4.6 mm) RP C18 column with 5 µm-sized particles. Ethanol and ethyl acetate (83:17 v/v) made up the greener eluent system, which was pumped at a flow speed of 1.0 mL/min. At a wavelength of 425 nm, CCM was detected. The greener HPLC methodology was linear in the 1–100 µg/mL range, with a determination coefficient of 0.9983. The greener HPLC methodology for CCM estimation was also rapid (Rt = 3.57 min), accurate (%recoveries = 98.90–101.85), precise (%CV = 0.90–1.11), and sensitive (LOD = 0.39 µg/mL and LOQ = 1.17 µg/mL). The AGREE approach predicted the AGREE score of 0.81 for the established HPLC technique, indicating an outstanding greenness profile. The utility of the greener HPLC methodology was demonstrated by determining CCM in the in-house developed nanoemulsion, Curcuma longa extract, and commercial tablets. The % amount of CCM in the in-house developed nanoemulsion, Curcuma longa extract, and commercial tablets was found to be 101.24%, 81.15%, and 78.41%, respectively. The greener HPLC methodology was able to detect its degradation product under various stress conditions, suggesting its stability-indication characteristics. These results suggested that CCM in developed nanoemulsion, plant extract samples, and commercial tablets may be routinely determined using the greener HPLC methodology.

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Induced Cell Death as a Possible Pathway of Antimutagenic Action

Cited by 3 publications

References 143 publications

Antimutagenic Effects of the Composition from Green Tea Leaves Extracts and Caucasian Persimmon Fruits

Antimutagenic Effects of the Composition from Green Tea Leaves Extracts and Caucasian Persimmon Fruits

Enzymatic Hydrolysis of Rutin: Evaluation of Kinetic Parameters and Anti-Proliferative, Mutagenic and Anti-Mutagenic Effects

Greener Stability-Indicating HPLC Approach for the Determination of Curcumin in In-House Developed Nanoemulsion and Curcuma longa L. Extract

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