Induced-differential changes on lignan and phenolic acid compounds in Linum album hairy roots by fungal extract of Piriformospora indica

Tashackori, Hannaneh; Sharifi, Mohsen; Chashmi, Najmeh Ahmadian; Safaie, Naser; Behmanesh, Mehrdad

doi:10.1007/s11240-016-1041-2

Cited by 38 publications

(18 citation statements)

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“…In hairy root cultures of Linum album lignan accumulation was successfully enlarged by fungal elicitor usage, and lariciresinol content rose threefold over control up to 260 µg/g DW (Bahabadi et al 2014). Similary Tashackori et al (2016), examining various lignan production in L. album hairy roots elicited with mycelium extract of Piriformospora indica, reported an 1.6-fold increase (up to 157.3 µg/g DW) over control in lariciresinol content. Chashimi et al (2016) demonstrated that coniferyl aldehyde (2 mM) significantly stimulated larici-and pinoresinol production up to 14.8-and 8.7-fold, respectively.…”

Section: Tablementioning

confidence: 84%

“…Lignans, such as podophyllotoxin and 5-methoxypodophyllotoxin, which are of special interest, since their derivatives etoposide and teniposide are used in cancer therapy (Bohlin and Rosén 1996), could be accumulated in considerable amounts in in vitro cultures of various species from Podophyllum and Linum genera (Petersen and Alfermann 2001;Fuss 2003;Majumder and Jha 2009). The enhancement of lignan production in in vitro culture systems of various species was achieved by optimization of culture conditions including elaboration of the most suitable for product improvement carbon source, phytohormones, culture conditions like light or dark regime, elicitor treatments (reviewed in Fuss 2003), developing of hairy root cultures (Kumar et al 2012;Bahabadi et al 2014;Wawrosch et al 2014;Gabr et al 2016Gabr et al , 2017Tashackori et al 2016;Chashimi et al 2016) and metabolic engineering approaches (reviewed in Satake et al 2015). There has been also one paper on in vitro cultivated Taxus x media cell suspension and shoots where pinoresinol has been determined and revealed solely in shoots (Mistrzak et al 2015).…”

Section: Introductionmentioning

confidence: 99%

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Lignan accumulation in two-phase cultures of Taxus x media hairy roots

Sykłowska-Baranek

Łysik

Jeziorek

et al. 2018

Plant Cell Tiss Organ Cult

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The biosynthetic potential for six lignans accumulation in two lines of Taxus x media hairy roots was investigated. The cultures of KT and ATMA hairy root lines were supplemented with precursors: coniferyl alcohol (CA 1, 10 or 100 µM) and/or l-phenylalanine (100 µM PHEN) and/or methyl jasmonate (100 µM MeJa). Moreover the two-phase in vitro cultures supported with perfluorodecalin (PFD) as a gas carrier and in situ extrahent were used. The hairy root lines differed in lignan production profiles. In the control untreated cultures KT roots did not accumulate secoisolariciresinol and lariciresinol while ATMA roots did not accumulate matairesinol. In ATMA roots the treatment with CA (1 or 10 µM) resulted in the production of lariciresinol and secoisolariciresinol whereas solely lariciresinol was present after 100 µM CA application. Elicitation with 1 µM CA and MeJa yielded with hydroxymatairesinol aglyca and lariciresinol glucosides with their highest content 37.88 and 3.19 µg/g DW, respectively. The stimulatory effect of simultaneous treatment with 1 µM CA, PHEN and MeJa on lignan production was observed when the cultures were supplemented with PFD-aerated or degassed. In ATMA root cultures these applied conditions were the most favourable for matairesinol content which amounted to 199.86 and 160.25 µg/g DW in PFD-aerated and PFD-degassed supported cultures, respectively. In KT root cultures solely, hydroxymatairesinol and coniferin/CA content was enhanced with their highest yield 59.29 and 134.60 µg/g DW in PFD-aerated and PFD-degassed cultures, respectively.

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Section: Tablementioning

confidence: 84%

Section: Introductionmentioning

confidence: 99%

Lignan accumulation in two-phase cultures of Taxus x media hairy roots

Sykłowska-Baranek

Łysik

Jeziorek

et al. 2018

Plant Cell Tiss Organ Cult

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“…The addition of 3% (v/v) of P. indica cell homogenate significantly enhanced the cell growth (1.11 times) as compared to the untreated control. Similarly, hairy root lines generated from Linum album were treated with fungal extracts of Piriformospora indica, and a short-term exposure to fungal extract increased the hairy root biomass by 1.5-fold (Tashackori et al 2016). Fungal elicitors have been explored for plant secondary metabolites enhancement in plant cell suspension cultures.…”

Section: Fungal and Yeast Elicitormentioning

confidence: 99%

Biotic elicitors enhance diosgenin production in Helicteres isora L. suspension cultures via up-regulation of CAS and HMGR genes

Shaikh

Shriram

Khare

et al. 2020

Physiol Mol Biol Plants

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In an attempt to find an alternative and potent source of diosgenin, a steroidal saponin in great demand for its pharmaceutical importance, Helicteres isora suspension cultures were explored for diosgenin extraction. The effect of biotic elicitors on the biosynthesis of diosgenin, in suspension cultures of H. isora was studied. Bacterial as well as fungal elicitors such as Escherichia coli, Bacillus subtilis, Saccharomyces cerevisiae and Aspergillus niger were applied at varying concentrations to investigate their effects on diosgenin content. The HPLC based quantification of the treated samples proved that amongst the biotic elicitors, E. coli (1.5%) proved best with a 9.1-fold increase in diosgenin content over respective control cultures. Further, the scaling-up of the suspension culture to shake-flask and ultimately to bioreactor level were carried out for production of diosgenin. During all the scaling-up stages, diosgenin yield obtained was in the range between 7.91 and 8.64 mg l -1 , where diosgenin content was increased with volume of the medium. The quantitative real-time PCR (qRT-PCR) analysis showed biotic elicitors induced the expression levels of regulatory genes in diosgenin biosynthetic pathway, the 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) and cycloartenol synthase (CAS), which can be positively correlated with elicited diosgenin contents in those cultures. The study holds significance as H. isora represents a cleaner and easy source of diosgenin where unlike other traditional sources, it is not admixed with other steroidal saponins, and the scaled-up levels of diosgenin achieved herein have the potential to be explored commercially.

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“…To date, most of the studies focusing on L. album have been designed to enhance lignan compounds in in vitro cultures. Various techniques, such as optimizing the culture medium [46], the use of elicitors [47], or inducing polyploidy [48], have been successful in increasing the accumulation of lignans in various L. album in vitro culture. However, insufficient research has been conducted under greenhouse or field conditions.…”

Section: Introductionmentioning

confidence: 99%

Morphological, Physiological, and Biochemical Impacts of Different Levels of Long-Term Water Deficit Stress on Linum album Ky. ex Boiss. Accessions

et al. 2020

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Linum album (Ky. ex Boiss.) is an important medicinal plant that produces compounds such as the well-known anticancer lignan podophyllotoxin and fatty acids. Despite its high medicinal value, it has not yet been studied in detail under agricultural conditions. This study was conducted to evaluate the morphological, phenological, and physiological responses of six L. album accessions under different levels of water deficit treatments (100%, 75%, 50%, and 25% available water) in pot conditions. Based on the results, some of the morphological characteristics of the response to water deficit were established. Accessions UTLA7, UTLA9, and UTLA10 showed a higher seed yield and dry weight of the vegetative part. There was a substantial difference in the occurrence of phenological stages in the accessions. The maturation process was accelerated in plants under stress conditions, and accession UTLA9 completed its complete growth cycle faster than the other accessions. The physiological responses of the different accessions did not show the same pattern on the basis of the characteristics studied, and significant differences were observed depending on the trait and accession. Among the most important results of this study was the diversity of responses in different accessions. Based on these results, it is recommended that morphological features (such as seed yield per plant, plant height, number of inflorescences per plant, shoot and root dry weight) be used to select tolerant accessions for the desired product.

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Induced-differential changes on lignan and phenolic acid compounds in Linum album hairy roots by fungal extract of Piriformospora indica

Cited by 38 publications

References 38 publications

Lignan accumulation in two-phase cultures of Taxus x media hairy roots

Lignan accumulation in two-phase cultures of Taxus x media hairy roots

Biotic elicitors enhance diosgenin production in Helicteres isora L. suspension cultures via up-regulation of CAS and HMGR genes

Morphological, Physiological, and Biochemical Impacts of Different Levels of Long-Term Water Deficit Stress on Linum album Ky. ex Boiss. Accessions

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